| Place | Time | Swimmer (Grade) | School | City | State |
| 1 | :53.33 | Debroah Roth (11) | Redwood High School | Larkspur | CA |
| 2 | :53.48 | Mary Hanson (11) | Immanuel Christian | Ridgecrest | CA |
| 3 | :53.55 | Kate McNeilis (11) | Dublin Scioto High School | Dublin | OH |
| 4 | :53.66 | Elizabeth Beisel (11) | North Kingstown High School | North Kingstown | RI |
| 5 | :53.71 | Erin Reisinger (11) | Walton High School | Marietta | GA |
| 6 | :53.73 | Cindy Tran (11) | Edison High School | Huntington Beach | CA |
| 7 | :53.84 | Aubrey Peacock (11) | The Bolles School | Jacksonville | FL |
| 8 | :53.89 | Mary Beck (12) | Westlake High School | Austin | TX |
| 8 | :53.89 | Arden Pitman (10) | The Baylor School | Chattanooga | TN |
| 10 | :53.95 | Annemarie Thayer (10) | Ballard High School | Seattle | WA |
| 11 | :53.98 | Kim Holden (12) | Fox Lane High School | Bedford | NY |
| 12 | :54.05 | Emily Bos (11) | Holland High School | Holland | MI |
| 13 | :54.07 | Rachel Bootsma (9 ) | Eden Prairie High School | Eden Prairie | MN |
| 14 | :54.09 | Taylor Wohrley (12) | Timberland High School | Wentzville | MO |
| 15 | :54.12 | Megan Romano (12) | Northeast High School | St Petersburg | FL |
| 16 | :54.21 | Yumi So (12) | Crescenta Valley High School | La Crescenta | CA |
| 17 | :54.45 | Lauren Driscoll (9 ) | American Heritage School | Plantation | FL |
| 17 | :54.45 | Paige Whitmire (12) | Souderton Area High School | Souderton | PA |
| 19 | :54.54 | Sarah Denninghoff (11) | Sabino High School | Tucson | AZ |
| 20 | :54.65 | Bonnie Brandon (9 ) | Cherry Creek High School | Greenwood Village | CO |
| 21 | :54.70 | Jillian Vitarius (9 ) | Kingwood High School | Kingwood | TX |
| 22 | :54.74 | Megan Lafferty (12) | Upper Dublin High School | Ft. Washington | PA |
| 23 | :54.77 | Jana Mangimelli (12) | Brookwood High School | Snellville | GA |
| 24 | :54.84 | Chelsea Bailey (10) | Kentlake High School | Kent | WA |
| 25 | :54.85 | Meredith Cavalier (12) | Brooke Point High School | Stafford | VA |
| 26 | :54.98 | Chelsea Griffiths (12) | William S. Hart High School | Newhall | CA |
| 27 | :55.09 | Jessie Hammes (12) | Carmel High School | Carmel | IN |
| 28 | :55.24 | Cara Chuang (11) | Poolesville High School | Poolesville | MD |
| 29 | :55.26 | Ashley Cohagen (12) | Ann Arbor Pioneer High School | Ann Arbor | MI |
| 30 | :55.30 | Krissie Brandenburg (11) | Beechwood High School | Fort Mitchell | KY |
| 30 | :55.30 | Allie Roberts (10) | Emmaus High School | Emmaus | PA |
| 32 | :55.31 | Laurence Delisle (12) | Archbishop Mitty High School | San Jose | CA |
| 33 | :55.37 | Kendyl Stewart (9 ) | La Costa Canyon High School | Carlsbad | CA |
| 34 | :55.38 | Ellen Williamson (10) | Notre Dame Academy | Park Hills | KY |
| 35 | :55.41 | Kim Pavlin (11) | Churchill High School | San Antonio | TX |
| 36 | :55.42 | Tess Behrens (11) | Maple Grove Senior High | Maple Grove | MN |
| 36 | :55.42 | Lily Moldenhauer (11) | Churchill High School | San Antonio | TX |
| 36 | :55.42 | Samantha Taylor (11) | Capistrano Valley High School | Mission Viejo | CA |
| 39 | :55.43 | Shirley Wang (10) | Plano Senior High School | Plano | TX |
| 40 | :55.47 | Ashley Brewer (11) | Chaparral High School | Scottsdale | AZ |
| 41 | :55.48 | Laurel Chaney (12) | Bearden High School | Knoxville | TN |
| 42 | :55.51 | Danielle Smith (11) | Boca Raton High School | Boca Raton | FL |
| 43 | :55.55 | Ellen Lobb (11) | Clear Lake High School | Houston | TX |
| 44 | :55.56 | Jane Munro (11) | Evanston Township High School | Evanston | IL |
| 45 | :55.60 | Lauren Solernou (11) | Sacred Heart High School | Waterbury | CT |
| 46 | :55.63 | Bailey Beins (12) | Wichita Heights High School | Wichita | KS |
| 47 | :55.68 | Melanie Busch (10) | William Tennent High School | Warminster | PA |
| 47 | :55.68 | Erin Cameron (11) | Carlson High School | Gibraltar | MI |
| 47 | :55.68 | Ashlee Linn (9 ) | Riverview High School | Sarasota | FL |
| 50 | :55.70 | Sarah Galvin (12) | Benet Academy | Lisle | IL |
| 50 | :55.70 | Natilee Ruiz (9 ) | Othello High School | Othello | WA |
| 52 | :55.72 | Stephanie Wei (12) | Archbishop Mitty High School | San Jose | CA |
| 53 | :55.73 | Karen Wang (11) | Foothill High School | Pleasanton | CA |
| 54 | :55.74 | Lauren Piper (11) | Riverview High School | Sarasota | FL |
| 55 | :55.81 | Lauren Hines (12) | Hidden Valley High School | Roanoke | VA |
| 55 | :55.81 | Dagny Knutson (11) | Minot High School | Minot | ND |
| 57 | :55.86 | Melanie McClure (11) | Mansfield High School | Mansfield | TX |
| 58 | :55.88 | Kyra Jones (11) | Seven Lakes High School | Katy | TX |
| 59 | :55.92 | Patton Shelly (9 ) | Heritage High School | Littleton | CO |
| 60 | :55.93 | Haley Smith (12) | Blue Valley North High School | Overland Park | KS |
| 61 | :55.99 | Elisabeth Tavierne (12) | Rosary High School | Aurora | IL |
| 62 | :56.00 | Andrea Marko (11) | Greensburg Central Catholic High School | Greensburg | PA |
| 63 | :56.10 | Jackie Powell (11) | Madison Memorial High School | Madison | WI |
| 64 | :56.11 | Andrea Basaraba (11) | The Bishop's School | San Diego | CA |
| 64 | :56.11 | Berklie Oscarson (11) | San Marino High School | San Marino | CA |
| 66 | :56.12 | Kathryn Eckhart (9 ) | Cypress Creek High School | Houston | TX |
| 67 | :56.14 | Brooke Waugaman (10) | Hempfield Area High School | Greensburg | PA |
| 68 | :56.15 | Emily Creran (12) | Haddonfield Memorial High School | Haddonfield | NJ |
| 68 | :56.15 | Danielle Harris (12) | Valley High School | West Des Moines | IA |
| 68 | :56.15 | Tiffany Van Dongen (12) | Klein Collins High School | Spring | TX |
| 71 | :56.17 | Hailey Campbell (11) | Lakeville South High School | Lakeville | MN |
| 72 | :56.19 | Morgan Sprosty (11) | Northwood High School | Irvine | CA |
| 73 | :56.21 | Eva Greene (10) | McLean High School | McLean | VA |
| 73 | :56.21 | Marissa Neel (9 ) | San Ramon Valley High School | Danville | CA |
| 75 | :56.24 | Kasey Morris (11) | Oscar Smith High School | Chesapeake | VA |
| 76 | :56.25 | Katherine Huff (9 ) | Lakeside High School | Evans | GA |
| 77 | :56.26 | Ruthie Norval (11) | Westlake High School | Austin | TX |
| 78 | :56.27 | Stephanie Kinsey (11) | Trinity High School | Camp Hill | PA |
| 79 | :56.28 | Rachel O'Brien (12) | College Park High School | Pleasant Hill | CA |
| 80 | :56.33 | Aubrey Hertzler (11) | Hamilton Southeastern High School | Fishers | IN |
| 81 | :56.37 | Shelbi Luchini (12) | Campolindo High School | Moraga | CA |
| 82 | :56.39 | Allison Kerry Sullivan (12) | Upper Arlington High School | Upper Arlington | OH |
| 83 | :56.41 | Kirsten Milberg (12) | Rock Canyon High School | Highlands Ranch | CO |
| 84 | :56.42 | Jennifer Warmolts (12) | Upper Arlington High School | Upper Arlington | OH |
| 85 | :56.45 | Kelsey Klatt (12) | Sunnyside High School | Fresno | CA |
| 86 | :56.46 | Hannah Freyman (10) | Richwoods High School | Peoria | IL |
| 87 | :56.47 | Lisa Boyce (11) | University of Illinois Laboratory High School | Urbana | IL |
| 88 | :56.48 | Courtney Ciesielski (11) | Lassiter High School | Marietta | GA |
| 89 | :56.54 | Emily Andelson (12) | Palm Desert High School | Palm Desert | CA |
| 89 | :56.54 | Lauren Damian (10) | Monte Vista High School | Danville | CA |
| 91 | :56.55 | Alexa Powers (12) | Carondelet High School | Concord | CA |
| 92 | :56.57 | Alicia Grima (9 ) | Woodside High School | Woodside | CA |
| 93 | :56.59 | Kearsten Livingstone (11) | Canyon High School | Canyon Country | CA |
| 94 | :56.60 | Jesse Bergman (11) | Homestead High School | Cupertino | CA |
| 94 | :56.60 | Maegan O'Connor (12) | Hamilton Southeastern High School | Fishers | IN |
| 96 | :56.61 | Kaylin Bing (10) | Roseburg High School | Roseburg | OR |
| 97 | :56.62 | Courtney Bartholomew (9 ) | Holland High School | Holland | MI |
| 98 | :56.65 | Molly Albrecht (12) | Lawrence Free State High School | Lawrence | KS |
| 99 | :56.67 | Wylie Escobedo (11) | Austin High School | Austin | TX |
| 100 | :56.69 | Riley Flanagan (11) | Buchholz High School | Gainesville | FL |
| 101 | :56.72 | Taylor Foster (11) | Breck High School | Minneapolis | MN |
| 102 | :56.77 | Alena Kluge (11) | Emmaus High School | Emmaus | PA |
| 102 | :56.77 | Callie Nettles (11) | North Cobb High School | Kennesaw | GA |
| 102 | :56.77 | Morgan Smith (12) | Klein High School | Klein | TX |
| 102 | :56.77 | Caroline Smith (12) | South Davidson High School | Denton | NC |
| 106 | :56.81 | Anna DeMonte (9 ) | Ann Arbor Huron High School | Ann Arbor | MI |
| 106 | :56.81 | Claudia Doyle (10) | Thomas Worthington High School | Worthington | OH |
| 108 | :56.83 | Kelsey Murtagh (12) | Greencastle High School | Greencastle | IN |
| 109 | :56.84 | Jamie Barrett (12) | Butler High School | Vandalia | OH |
| 109 | :56.84 | Lolo Blair (12) | Santa Barbara High School | Santa Barbara | CA |
| 111 | :56.87 | Brienne Ryan (12) | Cornwall Central High School | New Windsor | NY |
| 112 | :56.89 | Michelle Gean (11) | Kingwood High School | Kingwood | TX |
| 112 | :56.89 | Kelly Ryan (11) | Fenwick High School | Oak Park | IL |
| 114 | :56.91 | Alyssa Busch (12) | United Township High School | East Moline | IL |
| 114 | :56.91 | Margo Stanfa (12) | Rockford Guilford High School | Rockford | IL |
| 116 | :56.92 | Lindsay Zeberlein (12) | Mountain Vista High School | Highlands Ranch | CO |
| 117 | :56.94 | Katrina Anderson (12) | Granada High School | Livermore | CA |
| 118 | :56.98 | Emma Szczupakiewicz (9 ) | Muskego High School | Muskego | WI |
| 119 | :56.99 | Stephanie Nguyen (11) | Lynbrook High School | San Jose | CA |
| 120 | :57.05 | Chloe Portela (10) | Lawrence Free State High School | Lawrence | KS |
| 121 | :57.10 | Sophie Allen (12) | Boulder High School | Boulder | CO |
| 121 | :57.10 | Larah Beaver (9 ) | Yorktown High School | Yorktown | IN |
| 121 | :57.10 | Carlotta Fiordoro (11) | Miami Beach High School | Miami Beach | FL |
| 121 | :57.10 | Chrissy Thomas (12) | Las Lomas High School | Walnut Creek | CA |
| 125 | :57.12 | Rebecca Stoughton (10) | Peoria Notre Dame High School | Peoria | IL |
| 126 | :57.13 | Kellyn Angell (12) | Harpeth Hall School | Nashville | TN |
| 127 | :57.15 | Caitlyn Forman (9 ) | Notre Dame Academy | Park Hills | KY |
| 128 | :57.16 | Megan Jex (11) | Carmel High School | Carmel | IN |
| 129 | :57.20 | Mallory Miles (11) | Southridge High School | Huntingburg | IN |
| 130 | :57.22 | Taylor Kitayama (11) | Fairview High School | Boulder | CO |
| 131 | :57.23 | Katie Nolan (12) | Hershey High School | Hershey | PA |
| 132 | :57.26 | Kathryn Haller (12) | Mentor High School | Mentor | OH |
| 132 | :57.26 | Lauren Jordan (10) | Carmel High School | Carmel | IN |
| 134 | :57.27 | Kellie Darmody (12) | Providence High School | Charlotte | NC |
| 134 | :57.27 | Kelly Dunn (10) | West Chicago Community High School | West Chicago | IL |
| 136 | :57.28 | Spencer Chappell (10) | Germantown High School | Germantown | TN |
| 136 | :57.28 | Rachel Moore (9 ) | Andover High School | Andover | MA |
| 138 | :57.29 | Kaitlyn Lo (11) | Sir Francis Drake High School | San Anselmo | CA |
| 139 | :57.30 | Kellie Barbiea (10) | Springboro High School | Springboro | OH |
| 139 | :57.30 | Alexandra Huff (10) | Wakeland High School | Frisco | TX |
| 139 | :57.30 | Molly Smyth (10) | Cromwell High School | Cromwell | CT |
| 142 | :57.31 | Emily Harris (12) | Webster Groves High School | Webster Groves | MO |
| 142 | :57.31 | Amy Katz (10) | North Central High School | Indianapolis | IN |
| 142 | :57.31 | Kati Kyle (11) | Newsome High School | Lithia | FL |
| 142 | :57.31 | Anji Shakya (11) | Davis Senior High School | Davis | CA |
| 146 | :57.32 | Kelsey Ferrante (10) | McDowell High School | Erie | PA |
| 146 | :57.32 | Alexa Harris (12) | Moline High School | Moline | IL |
| 146 | :57.32 | Libby Hunsche (11) | Turpin High School | Cincinnati | OH |
| 149 | :57.33 | Joann Liang (11) | Northgate High School | Walnut Creek | CA |
| 150 | :57.35 | Madeline Armitage (12) | Upper Arlington High School | Upper Arlington | OH |
| 150 | :57.35 | Shaya Schaedler (9 ) | Campbell County High School | Gillette | WY |
| 152 | :57.36 | Jenna Young (11) | New Albany High School | New Albany | OH |
| 153 | :57.39 | Rachel Flinn (11) | Peddie School | Hightstown | NJ |
| 154 | :57.40 | Lauren Rosenberg (11) | Olentangy Liberty High School | Powell | OH |
| 155 | :57.41 | Paige Impink (11) | Wilson High School | West Lawn | PA |
| 156 | :57.45 | Kelsea Cross (12) | Columbus North High School | Columbus | IN |
| 156 | :57.45 | Ashleigh Ferguson (9 ) | Academy of the Holy Cross | Kensington | MD |
| 156 | :57.45 | Taylor Sneed (10) | Germantown Academy | Ft. Washington | PA |
| 159 | :57.46 | Sarah Cruzan (11) | Southridge High School | Beaverton | OR |
| 160 | :57.47 | Lauren Opatrny (11) | Saratoga High School | Saratoga | CA |
| 161 | :57.48 | Rebecca Dugan (10) | Germantown Academy | Ft. Washington | PA |
| 162 | :57.49 | Allie Sullins (9 ) | Magnolia West High School | Magnolia | TX |
| 163 | :57.50 | Hayley Johnson (10) | Marian High School | Bloomfiled Hills | MI |
| 163 | :57.50 | Anna Neumeister (10) | Pope High School | Marietta | GA |