| Place | Time | Swimmer (Grade) | School | City | State |
| 1 | :52.83 | Lily Moldenhauer (11) | Churchill High School | San Antonio | TX |
| 2 | :52.99 | Katie Nolan (12) | Hershey High School | Hershey | PA |
| 3 | :53.03 | Yumi So (12) | Crescenta Valley High School | La Crescenta | CA |
| 4 | :53.34 | Lindsey Rae Marchand (12) | Peninsula High School | Gig Harbor | WA |
| 5 | :53.38 | Darcy Fishback (12) | Upper Arlington High School | Upper Arlington | OH |
| 6 | :53.44 | Kendyl Stewart (9 ) | La Costa Canyon High School | Carlsbad | CA |
| 7 | :54.07 | Rachel O'Brien (12) | College Park High School | Pleasant Hill | CA |
| 8 | :54.19 | Maya DiRado (11) | Maria Carrillo High School | Santa Rosa | CA |
| 9 | :54.29 | Katelyne Herrington (11) | Tokay High School | Lodi | CA |
| 10 | :54.31 | Cindy Tran (11) | Edison High School | Huntington Beach | CA |
| 11 | :54.33 | Emma Drysdale (12) | Monta Vista High School | Cupertino | CA |
| 12 | :54.36 | Lauren Harrington (10) | St. Mary's Episcopal School | Memphis | TN |
| 13 | :54.38 | Caroline Kuczynski (12) | American Heritage School | Plantation | FL |
| 14 | :54.40 | Kim Holden (12) | Fox Lane High School | Bedford | NY |
| 15 | :54.47 | Kaitlin Frehling (11) | Seminole High School | Seminole | FL |
| 16 | :54.50 | Jessie Hammes (12) | Carmel High School | Carmel | IN |
| 17 | :54.52 | Casey Clark (10) | Klein High School | Klein | TX |
| 18 | :54.61 | Christina Bechtel (10) | Franklin High School | Franklin | TN |
| 19 | :54.69 | Mary Griffith (9 ) | Tokay High School | Lodi | CA |
| 19 | :54.69 | Alexandra Horn (10) | Cave Spring High School | Roanoke | VA |
| 19 | :54.69 | Dagny Knutson (11) | Minot High School | Minot | ND |
| 22 | :54.70 | Ashley Cohagen (12) | Ann Arbor Pioneer High School | Ann Arbor | MI |
| 22 | :54.70 | Caroline McElhany (12) | Friendswood High School | Friendswood | TX |
| 22 | :54.70 | Jillian Vitarius (9 ) | Kingwood High School | Kingwood | TX |
| 25 | :54.76 | Emma Goral (10) | Arrowhead High School | Hartland | WI |
| 26 | :54.77 | Dani Barbiea (10) | Springboro High School | Springboro | OH |
| 26 | :54.77 | Katherine Coleman (12) | Palm Harbor University High School | Palm Harbor | FL |
| 26 | :54.77 | Megan Fonteno (10) | The Bolles School | Jacksonville | FL |
| 29 | :54.82 | McKenzie Bagan (12) | Homestead High School | Fort Wayne | IN |
| 30 | :54.86 | Megan Leung (11) | St. Ignatius College Preparatory | San Francisco | CA |
| 31 | :54.89 | Megan Fischer-Colbrie(12) | Monta Vista High School | Cupertino | CA |
| 32 | :54.92 | Chelsea Howard (11) | Riverside High School | Durham | NC |
| 33 | :54.94 | Kylie Garcia (11) | Rowland High School | Rowland Heights | CA |
| 33 | :54.94 | Kearsten Livingstone (11) | Canyon High School | Canyon Country | CA |
| 35 | :54.95 | Sara Haley (12) | Oak Ridge High School | el dorado hills | CA |
| 36 | :54.96 | Elizabeth Beisel (11) | North Kingstown High School | North Kingstown | RI |
| 37 | :54.98 | Marissa Neel (9 ) | San Ramon Valley High School | Danville | CA |
| 37 | :54.98 | Noelle Tarazona (10) | Claremont High School | Claremont | CA |
| 37 | :54.98 | Ellen Williamson (10) | Notre Dame Academy | Park Hills | KY |
| 40 | :54.99 | Zina Grogg (11) | Moon Area High School | Moon Township | PA |
| 41 | :55.02 | Rachel Bootsma (9 ) | Eden Prairie High School | Eden Prairie | MN |
| 41 | :55.02 | Gabrielle Mizerak (12) | Osbourn Park High School | Manassas | VA |
| 43 | :55.04 | Stephanie Christofferson (12) | Marin Academy | San Rafael | CA |
| 44 | :55.05 | Ana Anaya (10) | John A Ferguson Senior High School | Miami | FL |
| 44 | :55.05 | Megan Lafferty (12) | Upper Dublin High School | Ft. Washington | PA |
| 46 | :55.07 | Ellen Lobb (11) | Clear Lake High School | Houston | TX |
| 47 | :55.09 | Morgan Sprosty (11) | Northwood High School | Irvine | CA |
| 47 | :55.09 | Seraphina Van (12) | Cypress Woods High School | Cypress | TX |
| 49 | :55.10 | Allyson Sweeney (12) | Brookwood High School | Snellville | GA |
| 49 | :55.10 | Rheanna Vaughn (11) | Aliso Niguel High School | Aliso Viejo | CA |
| 51 | :55.13 | Catherine Breed (10) | Amador Valley High School | Pleasanton | CA |
| 52 | :55.15 | Ali Polhill (11) | Brewster High School | Brewster | NY |
| 53 | :55.22 | Nikki Hubbard (11) | Penn High School | Mishawaka | IN |
| 54 | :55.23 | Carolyn Fittin (10) | Peddie School | Hightstown | NJ |
| 55 | :55.26 | Emily Andelson (12) | Palm Desert High School | Palm Desert | CA |
| 56 | :55.28 | Grace Tarka (12) | St. Ignatius College Preparatory | San Francisco | CA |
| 57 | :55.29 | Alexandra Norris (11) | Sycamore High School | Cincinnati | OH |
| 58 | :55.35 | Alyssa Tran (12) | Esperanza High School | Anaheim | CA |
| 59 | :55.36 | Laura Sogar (12) | The Prout School | Wakefield | RI |
| 60 | :55.37 | Lindsay Parrish (12) | Santa Barbara High School | Santa Barbara | CA |
| 61 | :55.39 | Lauren Scott (12) | Starr's Mill High School | Fayetteville | GA |
| 62 | :55.42 | Emily Adamczyk (12) | San Marino High School | San Marino | CA |
| 63 | :55.45 | Bailey Beins (12) | Wichita Heights High School | Wichita | KS |
| 63 | :55.45 | Annalise Colton (12) | Edina High School | Edina | MN |
| 65 | :55.47 | Kaitlin Pawlowicz (10) | Oakton High School | Vienna | VA |
| 66 | :55.48 | Kristina Parsons (12) | Redwood High School | Larkspur | CA |
| 67 | :55.49 | Lauren Weaver (11) | Colerain High School | Cincinnati | OH |
| 68 | :55.51 | Mackenzie Powers (11) | Rosary High School | Aurora | IL |
| 68 | :55.51 | Olivia Scott (11) | Rosary High School | Aurora | IL |
| 70 | :55.53 | Amanda Kendall (12) | Robinson Secondary School | Fairfax | VA |
| 70 | :55.53 | Rhiannon Sheets (10) | Carmel High School | Carmel | IN |
| 72 | :55.58 | Jackie Rzymek (12) | McDowell High School | Erie | PA |
| 73 | :55.60 | Kaylin Bing (10) | Roseburg High School | Roseburg | OR |
| 73 | :55.60 | Kelsey Floyd (12) | Tates Creek High School | Lexington | KY |
| 75 | :55.61 | Andrea Marko (11) | Greensburg Central Catholic High School | Greensburg | PA |
| 76 | :55.65 | Leticia Lelli (9 ) | Chaparral High School | Scottsdale | AZ |
| 77 | :55.67 | Elizabeth Hall (12) | Chantilly High School | Chantilly | VA |
| 77 | :55.67 | Maegan O'Connor (12) | Hamilton Southeastern High School | Fishers | IN |
| 79 | :55.69 | Taylor Curado (12) | Rock Canyon High School | Highlands Ranch | CO |
| 80 | :55.70 | Caroline Lukins (12) | Campolindo High School | Moraga | CA |
| 81 | :55.71 | Shannon Guy (11) | Millard West High School | Omaha | NE |
| 82 | :55.73 | Audrey Kumm (10) | Hutchinson High School | Hutchinson | MN |
| 82 | :55.73 | Heather Savage (12) | Canandaigua Academy | Canandaigua | NY |
| 84 | :55.74 | Dayna Gettel (9 ) | Plano Senior High School | Plano | TX |
| 85 | :55.75 | Allison Knight (11) | La Jolla High School | La Jolla | CA |
| 86 | :55.77 | Summer Stephens (10) | Sacred Heart Academy | Louisville | KY |
| 87 | :55.78 | Rachael Dudley (9 ) | Mill Creek High School | Hoschton | GA |
| 87 | :55.78 | Nikki Larson (9 ) | Edina High School | Edina | MN |
| 89 | :55.79 | Victoria Gillespie (11) | Nazareth Academy | Philadelphia | PA |
| 89 | :55.79 | Lindzi Smith (12) | Lassiter High School | Marietta | GA |
| 91 | :55.81 | Bridget Casey (10) | Germantown Academy | Ft. Washington | PA |
| 92 | :55.82 | Adrienne Bicek (12) | Downers Grove North High School | Downers Grove | IL |
| 92 | :55.82 | Julie Ogden (10) | Lawrence North High School | Indianapolis | IN |
| 92 | :55.82 | Allison Kerry Sullivan (12) | Upper Arlington High School | Upper Arlington | OH |
| 95 | :55.83 | Haley Sims (10) | Downers Grove North High School | Downers Grove | IL |
| 96 | :55.85 | Marybeth Hall (12) | Brighton High School | Brighton | MI |
| 97 | :55.86 | Elena Carvell (12) | Waubonsie Valley High School | Aurora | IL |
| 98 | :55.87 | Carina Kirchner (11) | Blue Springs High School | blue Springs | MO |
| 99 | :55.89 | Ellie Johnson (12) | Mountain Ridge High School | Glendale | AZ |
| 100 | :55.95 | Brittany Robinson (9 ) | Wawasee High School | Syracuse | IN |
| 101 | :55.96 | Carissa Poeschel (12) | Franklin High School | Portland | OR |
| 102 | :55.97 | Alex Todd (10) | Regis Jesuit High School | Aurora | CO |
| 103 | :55.98 | Courtney Whyte (10) | Campolindo High School | Moraga | CA |
| 104 | :55.99 | Paige Copeland (11) | Hamilton Southeastern High School | Fishers | IN |
| 104 | :55.99 | Jackie Sherrard (12) | Scott High School | Taylor Mill | KY |
| 106 | :56.00 | Danielle Schulkin (11) | Walt Whitman High School | Bethesda | MD |
| 107 | :56.01 | Kathryn Ha (12) | Hackley School | Tarrytown | NY |
| 108 | :56.02 | Heather Coutts (10) | Bishop Kenny High School | Jacksonville | FL |
| 109 | :56.04 | Taylor Temple (10) | Hebron High School | Carrollton | TX |
| 110 | :56.05 | Molly Higgins (11) | Randolph High School | Randolph | NJ |
| 111 | :56.06 | Margaret Wenzlau (9 ) | Palo Alto High School | Palo Alto | CA |
| 112 | :56.07 | Callie Klein (10) | Monte Vista High School | Danville | CA |
| 112 | :56.07 | Courtney Otto (10) | West Seneca West Senior High School | West Seneca | NY |
| 112 | :56.07 | Treasa Ring (12) | Roseville Area High School | Roseville | MN |
| 115 | :56.08 | Kellyn Angell (12) | Harpeth Hall School | Nashville | TN |
| 115 | :56.08 | Emily Boone (10) | Bishop Watterson High School | Columbus | OH |
| 117 | :56.12 | Kelly Naze (9 ) | Cherry Creek High School | Greenwood Village | CO |
| 118 | :56.14 | Becca Soderholm (11) | Madison East High School | Madison | WI |
| 119 | :56.19 | Devon Mason (10) | Carmel High School | Carmel | IN |
| 120 | :56.21 | Anna Rae Gwarjanski (11) | The Baylor School | Chattanooga | TN |
| 121 | :56.22 | Sarah Lloyd (11) | Severna Park High School | Severna Park | MD |
| 122 | :56.24 | Jaclin Rehring (12) | Allen High School | Allen | TX |
| 123 | :56.26 | Allie Roberts (10) | Emmaus High School | Emmaus | PA |
| 124 | :56.27 | Emily Bos (11) | Holland High School | Holland | MI |
| 125 | :56.31 | Jackie Swartz (11) | Centerville High School | Centerville | OH |
| 126 | :56.34 | Lindsey Norberg (12) | Ithaca High School | Ithaca | NY |
| 127 | :56.35 | Jaclyn Amog (10) | Las Lomas High School | Walnut Creek | CA |
| 127 | :56.35 | Haley Holcombe (12) | Brookwood High School | Snellville | GA |
| 129 | :56.36 | Kelsey Amundsen (12) | The Woodlands High School | The Woodlands | TX |
| 129 | :56.36 | Maryn Bieganski (11) | Solon High School | Solon | OH |
| 129 | :56.36 | Charlotte Ruby (9 ) | Archbishop Mitty High School | San Jose | CA |
| 129 | :56.36 | Kate VanFossen (11) | Upper Arlington High School | Upper Arlington | OH |
| 133 | :56.38 | Emily Neubert (10) | Midway High School | Waco | TX |
| 133 | :56.38 | Carter Stephens (12) | The Collegiate School | Richmond | VA |
| 135 | :56.39 | Anastasia Ivanova (11) | Stanton College Preparatory School | Jacksonville | FL |
| 136 | :56.41 | Kelsey Krauss (12) | St. Petersburg Catholic High | St. Petersburg | FL |
| 137 | :56.42 | Kelcy Perry (12) | Model Laboratory School | Richmond | KY |
| 138 | :56.44 | Erin Flanigan (10) | James Bowie High School | Austin | TX |
| 138 | :56.44 | Audrey Lawson (12) | Notre Dame Academy | Park Hills | KY |
| 138 | :56.44 | Taylor Streid (12) | Peoria Notre Dame High School | Peoria | IL |
| 141 | :56.45 | Marissa Murphy (11) | Forest Hills Central High School | Grand Rapids | MI |
| 142 | :56.46 | Lauren Rigg (11) | Fairview High School | Boulder | CO |
| 143 | :56.48 | Natalie Morris (10) | Olathe Northwest High School | Olathe | KS |
| 144 | :56.49 | Rachel Moore (9 ) | Andover High School | Andover | MA |
| 145 | :56.50 | Allison Gargalikis (10) | Carondelet High School | Concord | CA |
| 146 | :56.51 | Michea Reinitz (12) | Mt. Vernon High School | Mt. Vernon | IN |
| 147 | :56.52 | Ali Tomkinson (9 ) | Carlsbad High School | Carlsbad | CA |
| 147 | :56.52 | Hillary Yarosh (11) | Unionville High School | Kennett Square | PA |
| 149 | :56.55 | Anna Kokensparger (12) | Duchesne Academy | Omaha | NE |
| 149 | :56.55 | Christine E. Riddle (11) | Grapevine / Colleyville Heritage High School | Grapevine | TX |
| 149 | :56.55 | Jasmine Tosky (9 ) | Palo Alto High School | Palo Alto | CA |
| 149 | :56.55 | Kelsi Worrell (9 ) | Rancocas Valley Regional High School | Mt. Holly | NJ |
| 153 | :56.57 | Kaela Anderson (11) | Stillwater High School | Stillwater | MN |
| 154 | :56.59 | Elizabeth Pepper (10) | Walter Johnson High School | Bethesda | MD |
| 155 | :56.60 | Kristen Redmond (12) | Centennial High School | Roswell | GA |
| 155 | :56.60 | Deanna Sorenson (12) | Glendale High School | Springfield | MO |
| 157 | :56.63 | Beth Rogers (11) | Canton McKinley High School | Canton | OH |
| 158 | :56.66 | Ibby Simcox (9 ) | Blue Springs High School | Blue Springs | MO |
| 158 | :56.66 | Annemarie Thayer (10) | Ballard High School | Seattle | WA |
| 160 | :56.67 | Liz Braun (11) | Sprayberry High School | Marietta | GA |
| 160 | :56.67 | Riley Flanagan (11) | Buchholz High School | Gainesville | FL |
| 160 | :56.67 | Katie Miles (12) | Grapevine / Colleyville Heritage High School | Grapevine | TX |
| 163 | :56.68 | Alexa Powers (12) | Carondelet High School | Concord | CA |
| 163 | :56.68 | Danielle Reminger (12) | Wheaton Warrenville South | Wheaton | IL |
| 165 | :56.69 | Cimone Miner (12) | Xavier College Preparatory | Phoenix | AZ |
| 166 | :56.71 | Maggie Hanson (11) | William S. Hart High School | Newhall | CA |
| 166 | :56.71 | Erin Reisinger (11) | Walton High School | Marietta | GA |
| 168 | :56.73 | Leslie Hackler (12) | Centennial High School | Roswell | GA |
| 169 | :56.74 | Natalya Ares (9 ) | Winston Churchill High School | Potomac | MD |