| Place | Time | Swimmers (Grades) | School | City | State |
| 1 | 1:32.75 | Megan Detro (12), Jessie Hammes (12), Logan Mason (12), Trish Regan (12) | Carmel High School | Carmel | IN |
| 2 | 1:33.35 | Sloane Pitman (12), Emma Michaels (10), Alison Lusk (11), Arden Pitman (10) | The Baylor School | Chattanooga | TN |
| 3 | 1:33.54 | Kelsey Amundsen (12), Chelsey Eberwein (12), Erin Foster (10), Mary Foster (12) | The Woodlands High School | The Woodlands | TX |
| 4 | 1:33.63 | Ruby Martin (12), Ivy Martin (10), Becca Soderholm (11), Aja VanHout (10) | Madison East High School | Madison | WI |
| 5 | 1:33.88 | Kara Savegnago (12), Olivia Scott (11), Fayhee Kally (12), Hare Katherine (9 ) | Rosary High School | Aurora | IL |
| 6 | 1:34.16 | Missy Doll (11), Chloe Thomas (10), Angela Severn (12), Paige Whitmire (12) | Souderton Area High School | Souderton | PA |
| 7 | 1:34.24 | Kayla Hammerberg (10), Kristyn Fulcher (11), Madeline Armitage (12), Darcy Fishback (12) | Upper Arlington High School | Upper Arlington | OH |
| 8 | 1:34.58 | Chelsea Fong (11), Megan Fischer-Colbrie(12), Audrey Kwong (12), Emma Drysdale (12) | Monta Vista High School | Cupertino | CA |
| 9 | 1:34.74 | Aubrey Hertzler (11), Erin Kelley (12), Carly Mercer (11), Paige Copeland (11) | Hamilton Southeastern High School | Fishers | IN |
| 9 | 1:34.74 | Samantha Wierzbicki (11), Taylor Brew (9 ), Taylor Straub (10), Caitlyn Karr (10) | Wilson High School | West Lawn | PA |
| 11 | 1:34.80 | Melissa McMahon (11), Wendy Consoer (12), Natalie Herrild (12), Hailey Campbell (11) | Lakeville South High School | Lakeville | MN |
| 11 | 1:34.80 | Betsy Galenti (12), Monica Radecke (11), Torie Buberl (10), Hannah Bowen (10) | Stillwater High School | Stillwater | MN |
| 13 | 1:34.94 | Cynthia Fascella (11), Erika Lucas (11), Danica Fascella (11), Shelby Webber (10) | Fountain Valley High School | Fountain Valley | CA |
| 13 | 1:34.94 | Laura Slater (11), Kristina Parsons (12), Deborah Roth (11), Devon Demont (11) | Redwood High School | Larkspur | CA |
| 15 | 1:34.96 | Stephanie Eisenring (12), Lara Moseley (12), Siena DeVenuto (11), Rebecca Aiello (10) | Pine Crest School | Fort Lauderdale | FL |
| 16 | 1:35.05 | Jennifer Talbott (11), Paige Gilley (11), Caroline Hansen (10), Maddy Schaefer (10) | Saint Francis High School | Mountain View | CA |
| 17 | 1:35.29 | Megan Marchuk (10), Allison Siegel (11), Kate Shumway (12), Maddy Urban (11) | New Trier High School | Winnetka | IL |
| 18 | 1:35.34 | Meghann McGoldrick (12), Kara Ploetz (11), Becca Ploetz (12), Lily Moldenhauer (11) | Churchill High School | San Antonio | TX |
| 19 | 1:35.38 | Mikelle Masciantonio (11), Deidre Geroni (12), Alena Kluge (11), Catherine Carr (11) | Emmaus High School | Emmaus | PA |
| 20 | 1:35.46 | Samantha Tucker (11), Camille Hendrix (12), Catherine Anne Prideaux (9 ), Katelin Scarsella (12) | Westlake High School | Austin | TX |
| 21 | 1:35.51 | Katelyn Miller (10), Samantha Rapp (11), Kara VanBlargan (10), Katie Nolan (12) | Hershey High School | Hershey | PA |
| 22 | 1:35.59 | Erin Reisinger (11), Adrienne Pilchard (11), Mary Rose Palermo (11), Jessica Graber (11) | Walton High School | Marietta | GA |
| 23 | 1:35.61 | Elizabeth McGinn (10), Shannon Guy (11), Bianca Chrsitensen (12), Emma O'Connell (10) | Millard West High School | Omaha | NE |
| 24 | 1:35.69 | Maureen McLaine (12), Meagan Hairston (12), Courtney Hubbard (12), Brooke Hawley (10) | Plano West Senior High School | Plano | TX |
| 25 | 1:35.70 | Alli Gillespie (10), Kiersten Colesen (12), Monique Wilson (12), Danielle Warde (11) | Edison High School | Huntington Beach | CA |
| 26 | 1:35.74 | Sara Delay (11), Jaymee Krochka (11), Tittle Mia (11), Joann Liang (11) | Northgate High School | Walnut Creek | CA |
| 27 | 1:35.84 | Lisa Swank (10), Jamie Friderichs (9 ), Michelle Gean (11), Dannye Osburn (12) | Kingwood High School | Kingwood | TX |
| 28 | 1:35.89 | Annalise Colton (12), Nikki Larson (9 ), Sarah Kemp (11), Mary Van Metre (11) | Edina High School | Edina | MN |
| 29 | 1:35.99 | Sydney Schaefer (12), Meredith Sweeney (11), Caitlin Connor (12), Sara Shaner (11) | Hinsdale Central High School | Hinsdale | IL |
| 30 | 1:36.02 | Kelsey Gouge (9 ), Haley Holcombe (12), Anna Springer (11), Allyson Sweeney (12) | Brookwood High School | Snellville | GA |
| 31 | 1:36.07 | Casey Sandlin (12), Ashely Butler (11), Gwen Vanden Noort (11), Taylor Josserand (11) | The Bolles School | Jacksonville | FL |
| 32 | 1:36.14 | Lindzi Smith (12), Shannon McLellan (12), Kim Carducci (10), Samantha Bosma (10) | Lassiter High School | Marietta | GA |
| 33 | 1:36.15 | Brittany Fozkos (11), Shauna Foor (11), Milana Socha (11), Mallory Przybylinski (12) | Chesterton High School | Chesterton | IN |
| 34 | 1:36.23 | Maureen Barron (9 ), Mariclaire Bresnahan (12), Morgan Bonk (12), Kelly Ryan (11) | Fenwick High School | Oak Park | IL |
| 35 | 1:36.37 | Mariah Hamil (9 ), Tori Bagan (10), Kristin Kinzer (12), Emily Keefer (12) | Homestead High School | Fort Wayne | IN |
| 36 | 1:36.45 | Gwen Comollo (12), Kara Dubiel (12), Courtney Raw (12), Megan Lydzinski (9 ) | Montgomery High School | Skillman | NJ |
| 37 | 1:36.50 | Lilly Marrow (11), Kara Parker (12), Katherine Maclean (12), Lauren McCleary (12) | Houston Stratford High School | Houston | TX |
| 37 | 1:36.50 | Paige Chilcoat (12), Casey Clark (10), Erica Dittmer (11), Katelyn Bain (10) | Klein High School | Klein | TX |
| 37 | 1:36.50 | Brittany Kent (12), Jennifer Miles (12), Kellie LaMothe (9 ), Lauren Massey (12) | North Central High School | Indianapolis | IN |
| 40 | 1:36.52 | Rachel Cleak (11), Andrea Ward (11), Olivia Alva (12), Dana Holt (10) | Campolindo High School | Moraga | CA |
| 41 | 1:36.54 | Tess Behrens (11), Heather Bodin (12), Lauren Kessler (11), Elaine Boosalis (10) | Maple Grove Senior High | Maple Grove | MN |
| 42 | 1:36.58 | Emily Brown (9 ), Hillary Petersen (11), Kirsten Soucek (11), Jamie Bohunicky (12) | Buchholz High School | Gainesville | FL |
| 43 | 1:36.59 | Shirin Sedaghat (12), Emily Scraggs (11), Tasia DeMuth (12), Rachael Cazden (12) | Fairview High School | Boulder | CO |
| 43 | 1:36.59 | Marley Cripe (10), Mackenzie Imbro (12), Claire Brolsma (9 ), Nora Brolsma (12) | Noblesville High School | Noblesville | IN |
| 45 | 1:36.60 | Ellyn Baumgardner (12), Frances Dowd (12), Jean DeOrnellas (12), Theresa Harvey (12) | Fairfax High School | Fairfax | VA |
| 46 | 1:36.67 | Gracie Finnegan (11), Alyson Francis (9 ), Michele Rielly (9 ), Camey Rabold (9 ) | Bishop Watterson High School | Columbus | OH |
| 46 | 1:36.67 | Erin Flanigan (10), Jessica Gardner (11), Koryn Ternes (9 ), Nadia El-Hamdi (10) | James Bowie High School | Austin | TX |
| 46 | 1:36.67 | Carolyn Meier (9 ), Erin Regan (10), Lindsey Schmidt (9 ), Megan Lafferty (12) | Upper Dublin High School | Ft. Washington | PA |
| 49 | 1:36.71 | Chloe Conjares (11), Danielle Romain (10), Lia Kunnapas (12), Bryeanne Ravettine (12) | Immaculate Heart Academy | Township of Washington | NJ |
| 50 | 1:36.76 | Danielle Harris (12), Kelsey Galles (11), Allison Harding (12), Lindsey LaMair (12) | Valley High School | West Des Moines | IA |
| 51 | 1:36.82 | Sierra Robbins (10), Meredith White (10), Jenna Haufler (9 ), Brooke Woodward (11) | Miramonte High School | Orinda | CA |
| 52 | 1:36.88 | Sato Kakihara (9 ), Megan Gegesky (12), Taylor Steffl (10), Nikki Barczak (10) | Mercy High School | Farmington Hills | MI |
| 53 | 1:36.92 | Morgan Barrows (12), Mariah Tharp (11), Mackenzie Schuler (10), Tristin Baxter (11) | Clovis West High School | Fresno | CA |
| 53 | 1:36.92 | Shaun Casey (10), Ginny Grams (10), Emily Grams (10), Kayla Derr (12) | Spruce Creek High School | Port Orange | FL |
| 55 | 1:36.94 | Haley Pietila (9 ), Tracy Vogel (11), Emma Goral (10), Holly Johnson (10) | Arrowhead High School | Hartland | WI |
| 56 | 1:36.97 | Sarah Liang (10), Sabrina Lee (10), Allie Bollella (12), Margaret Wenzlau (9 ) | Palo Alto High School | Palo Alto | CA |
| 57 | 1:37.02 | Emily Wright (11), Emily Downs (10), Olivia Blahut (12), Michelle Cunningham (10) | Wayland High School | Wayland | MA |
| 58 | 1:37.03 | Samantha Deana (11), Mary Morris (10), Heather Fellmeth (12), Nicole Dibonaventura (12) | North Penn High School | Lansdale | PA |
| 58 | 1:37.03 | Natalie Domeisen (10), Alexis Georgiadis (12), Katie Vincett (12), Margaux Georgiadis (11) | Shady Side Academy | Pittsburgh | PA |
| 60 | 1:37.09 | Sydney Saunders (11), Maggie Steffens (10), Kelly Mendoza (11), Ocean Trail (12) | Monte Vista High School | Danville | CA |
| 61 | 1:37.15 | Elizabeth Pitts (9 ), Meredith Nakano (10), Callie Northrop (10), Lauren Pfohl (12) | Marist School | Atlanta | GA |
| 62 | 1:37.16 | Valerie Barthelemy (12), Kristina Jaeger (12), Paige Kostishak (10), Hanna Cowley (10) | Ann Arbor Pioneer High School | Ann Arbor | MI |
| 63 | 1:37.17 | Nicole Cordero (9 ), Randi Windemuller (12), Haley Thompson (9 ), Caroline Meyer (12) | Holland High School | Holland | MI |
| 64 | 1:37.18 | Arianna Gorenc (12), Kathleen Keough (12), Elise Borja (11), Katie Nagel (12) | Waukesha South High School | Waukesha | WI |
| 65 | 1:37.20 | Allie Pennetti (9 ), Marina Rozick (10), Stephanie Graham (11), Kally Vanderbilt (9 ) | North Allegheny High School | Wexford | PA |
| 65 | 1:37.20 | Elena Carvell (12), Vicki Munson (11), Shelby Daeschner (12), Andrea Baumgartner (11) | Waubonsie Valley High School | Aurora | IL |
| 67 | 1:37.21 | Jesse Bergman (11), Kristen Clarno (9 ), Lisa Yao (12), Anne Peanasky (11) | Homestead High School | Cupertino | CA |
| 68 | 1:37.24 | Lauren Scott (12), Kristen Bates (11), Morgan Presley (9 ), Katie Haycraft (12) | Starr's Mill High School | Fayetteville | GA |
| 69 | 1:37.32 | Julia Messina (9 ), Brittney Kuras (12), Paula Bentley (10), Heather Savage (12) | Canandaigua Academy | Canandaigua | NY |
| 69 | 1:37.32 | M.E. Randall (11), Hope Curran (9 ), Renae DiBartolomeo (12), Rachel O'Brien (12) | College Park High School | Pleasant Hill | CA |
| 71 | 1:37.35 | Clark Cromer (11), Elly Faulkner (10), Summer Stephens (10), Rebecca Sears (9 ) | Sacred Heart Academy | Louisville | KY |
| 72 | 1:37.38 | Jordan Mattern (10), Jordyn Ambrosich (12), Katie Naze (11), Gretchen Cohen (11) | Cherry Creek High School | Greenwood Village | CO |
| 73 | 1:37.43 | Kinsey Kowalski (12), Cynthia Donovan (11), Lynn Brotherton (11), Breann McDowell (11) | Ursuline Academy | Cincinnati | OH |
| 74 | 1:37.44 | Emily Fogle (11), Victoria Waring (11), Erika Elliot (9 ), Kimmy Steelman (12) | Barrington High School | Barrington | IL |
| 74 | 1:37.44 | Sarah Koss (12), Kayla Effinger (9 ), Emily Moe (9 ), Kait Flederbach (11) | Westfield High School | Westfield | IN |
| 76 | 1:37.46 | Rebecca Reed (12), Kyle Krueger (11), Olivia Lin (12), Danielle Yoho (12) | Clements High School | Sugar Land | TX |
| 76 | 1:37.46 | Cari Caprio (11), Abbie Allen (10), Kayla Wiemers (11), Danielle Jansen (11) | Mason High School | Mason | OH |
| 78 | 1:37.49 | Michea Reinitz (12), Becca Slygh (11), Erika McCormick (9 ), Alex Norris (12) | Mt. Vernon High School | Mt. Vernon | IN |
| 79 | 1:37.54 | Xiao Ying Zhao (12), Rebecca Malone (12), Molly Mucciarone (9 ), Kathryn Raplee (12) | Peddie School | Hightstown | NJ |
| 80 | 1:37.56 | Erin Sheridan (9 ), Kristie Howard (11), MJ Mordell (12), Katie Howard (11) | Sacred Heart Prepararory | Atherton | CA |
| 81 | 1:37.62 | Meg Anderson (12), Hillary Hahmann (11), Haley Hahmann (10), Rebecca Werdine (9 ) | H.B. Plant High School | Tampa | FL |
| 82 | 1:37.63 | Cassidy Lapp (10), Rachael Ausdenmoore (12), Laura Huning (12), Brooke Morris (12) | Southlake Carroll High School | Southlake | TX |
| 83 | 1:37.64 | Alexa Morris (10), Devyn Phalen (12), Cori Gary (10), Colleen Konetzke (9 ) | Ronald Reagan High School | San Antonio | TX |
| 84 | 1:37.65 | Susan Bolt (12), Kate Dvornitsyn (11), Jillian Cordes (11), Kelly Hagerty (11) | Glenbrook South High School | Glenview | IL |
| 84 | 1:37.65 | Megan Leung (11), Grace Tarka (12), Claire Collins (11), Elizabeth Rosen (10) | St. Ignatius College Preparatory | San Francisco | CA |
| 84 | 1:37.65 | Sophie Pilhofer (12), Maddy DeMeules (11), Molly George (10), Meghan Weiss (12) | Visitation High School | Mendota Heights | MN |
| 87 | 1:37.67 | Catherine Cortesio (11), Allison Pavlik (9 ), Colleen Webb (11), Sarah Galvin (12) | Benet Academy | Lisle | IL |
| 88 | 1:37.70 | Jenna Young (11), Franciska Mandy (10), Fanny Mandy (10), Molly Dengler (12) | New Albany High School | New Albany | OH |
| 89 | 1:37.71 | Haley Smith (12), Kelsey Bakalar (12), Mardi Buchanan (10), Ana Kuehler (12) | Blue Valley North High School | Overland Park | KS |
| 90 | 1:37.72 | Melissa Cooper (11), Jessica Randolph (11), Chantal Bouthillier (9 ), Caroline Kuczynski (12) | American Heritage School | Plantation | FL |
| 91 | 1:37.73 | Taylor Guy (9 ), Nicole Skaggs (9 ), Ashlyn Acosta (11), Camille Hopp (12) | Mission Viejo High School | Mission Viejo | CA |
| 92 | 1:37.77 | Michelle Yakubisin (11), Meredith Gibbon (11), Holly Novacek (11), Ashley McAteer (12) | Penn-Trafford High School | Harrison City | PA |
| 93 | 1:37.78 | Ailey Ruger (12), Siobhan Hengemuhle (10), Meghan Varner (11), Sophie Allen (12) | Boulder High School | Boulder | CO |
| 94 | 1:37.80 | Kayley Dodson (11), Rachel Fattal (9 ), Darielle Watkins (10), Jessica Dunn (12) | Los Alamitos High School | Los Alamitos | CA |
| 95 | 1:37.83 | Meredith Colombo (11), Grace Raynor (11), Brianna Bilunas (10), Alex Bilunas (10) | Duchesne Academy | Omaha | NE |
| 96 | 1:37.85 | Samantha Moffitt (10), Skyler Doss (11), Paula Ferrara (9 ), Kathleen Gast (10) | Cedar Crest High School | Lebanon | PA |
| 96 | 1:37.85 | Alicia Berman (12), Shana Nameth (10), Rachel Hunsinger (12), Kathryn Haller (12) | Mentor High School | Mentor | OH |
| 98 | 1:37.86 | Grace Jarzemsky (11), Katie Delaney (10), Ashley Ryan (12), Kayla Beckwith (12) | Middleton High School | Middleton | WI |
| 99 | 1:37.87 | Stephanie Berg (11), Molly Schaefer (10), Jaynie Pulte (9 ), Maggie Brabbins (12) | Marian High School | Bloomfiled Hills | MI |
| 100 | 1:37.89 | Kiera Janze (10), Emily Schleif (9 ), Lauren Weis (12), Nicole Hutchins (9 ) | Cretin-Derham Hall High School | St. Paul | MN |
| 101 | 1:37.91 | Jackie Hodges (10), Rachel Jablonski (12), Lauren Sutton (10), Stefania Rossi (10) | Cinco Ranch High School | Katy | TX |
| 102 | 1:37.92 | Kathryn Haugen (12), Jennifer Lai (11), Jessica Del Monaco (9 ), Melissa Bates (11) | Mater Dei High School | Santa Ana | CA |
| 103 | 1:37.95 | Jessica Cutler (11), Jamie Van Portfliet (10), Mary Keeley (12), Kelsey Vermeer (12) | Rockford High School | Rockford | MI |
| 104 | 1:37.96 | Paige Duffy (12), Maryn Bieganski (11), Annie Marquiss (10), Emily Schwartz (11) | Solon High School | Solon | OH |
| 105 | 1:37.99 | Zoe Mattingly (9 ), Keely Simonson (9 ), Rachel Bewley (12), Alex Brennan (11) | Zionsville Community High School | Zionsville | IN |
| 106 | 1:38.00 | Katie McCullough (12), Hannah Messer (11), Marissa Murphy (11), Stephanie Bessinger (12) | Forest Hills Central High School | Grand Rapids | MI |
| 107 | 1:38.01 | Caroline Santinelli (11), Kathrine Wetherbee (10), Lauryn Brown (10), Christina Hurley (10) | Lincoln Sudbury Regional High School | Sudbury | MA |
| 108 | 1:38.02 | Brittany Rospierski (12), MacKenzie Harris (9 ), Dana Shaker (11), Alana Arnold (10) | Hawken School | Gates Mills | OH |
| 109 | 1:38.03 | Jackie Rzymek (12), Sarah Anderson (12), Lauren Szoszorek (10), Kelsey Herbst (12) | McDowell High School | Erie | PA |
| 109 | 1:38.03 | Amy Ballanfant (12), Ashley Douglas (11), Tessa Holmes (9 ), Ali Berry (12) | Mirabeau B. Lamar High School | Houston | TX |
| 111 | 1:38.04 | Victoria Baker (10), Hannah Walden (11), Rachel Canty (9 ), Amanda Kendall (12) | Robinson Secondary School | Fairfax | VA |
| 112 | 1:38.06 | Ashlee Linn (9 ), Lauren Piper (11), Jessica Piper (9 ), Cha Cha Bugatti (11) | Riverview High School | Sarasota | FL |
| 113 | 1:38.07 | Meg Vertovec (12), Megan Mc Nichols (12), Katherine Giger (12), Erika Ronning (11) | Lyons Township High School | La Grange | IL |
| 114 | 1:38.10 | Kellyn Angell (11), Kate Wiseman (10), Bridget Killian (9 ), Grace Anne Sanderson (12) | Harpeth Hall School | Nashville | TN |
| 114 | 1:38.10 | Melanie Rogers (11), Samantha Arnold (10), Charlotte Yaw (9 ), Lauren Nichols (12) | Seaholm High School | Birmingham | MI |
| 116 | 1:38.11 | Courtney Funk (9 ), Olsen Serena (11), Maggi Beck (12), Dagny Knutson (11) | Minot High School | Minot | ND |
| 117 | 1:38.14 | Sarah Haase (9 ), Morgan Dailey (10), Nora Considine (10), Blair Webb (12) | Our Lady of Good Counsel | Olney | MD |
| 118 | 1:38.15 | Eva Chen (10), Hilary Hunt (11), Dorothy Ren (9 ), Monica Garcia (11) | Archbishop Mitty High School | San Jose | CA |
| 119 | 1:38.18 | Jennifer Ryan (12), Devin Beahm (12), Emily Zheng (10), Susanne Gingher (10) | State College High School | State College | PA |
| 120 | 1:38.22 | Kayce Smith (9 ), Mary Van Leuven (9 ), Alissa Endres (11), Mackenzie King (12) | Madison Memorial High School | Madison | WI |
| 121 | 1:38.25 | Marissa Konicke (10), Natalie Sanchez (10), Chloe Farrar (12), Kelsey Konicke (12) | Plano Senior High School | Plano | TX |
| 122 | 1:38.26 | Emilie Kaufman (11), Hilary Langsam (12), Amanda Wilson (11), Tannhauser Kelly (11) | Juanita High School | Kirkland | WA |
| 123 | 1:38.30 | Randi Bosch (12), Andie Breslin (11), Lauren Leon (12), Patricia Leeson (11) | Liberty High School | Bethlehem | PA |
| 124 | 1:38.32 | Lauren Gustafson (12), Myra Retrum (9 ), Kristin VanDeventer (9 ), Ella Wildemann (10) | Columbus North High School | Columbus | IN |
| 124 | 1:38.32 | Catherine Wagner (11), Rachel Moody (10), Zoe Bond (10), Kristin Rollins (12) | Memorial High School | Houston | TX |
| 124 | 1:38.32 | Hillary Thomas (10), Taylor Debever (11), Casey Clearwater (10), Vanessa Pollitt (12) | Regis Jesuit High School | Aurora | CO |
| 124 | 1:38.32 | Stacy Coffyn (11), Katy Richardson (10), Taylor Burkhead (11), Marston Fries (9 ) | Shawnee Mission East | Shawnee Mission | KS |
| 128 | 1:38.33 | Iris Yan (11), Abigail Puczkowski (10), Marisa De Wames (12), Jackie Brousseau (12) | Westlake High School | Westlake Village | CA |
| 129 | 1:38.35 | Melissa Kander (12), Haley Bratys (10), Lauren Davidson (12), Julianne McNamee (12) | Dublin Jerome High School | Dublin | OH |
| 129 | 1:38.35 | Meghan Overend (10), Jessica Hawken (12), Jayme Katis (10), Megan Howard (10) | Langley High School | McLean | VA |
| 129 | 1:38.35 | Lambrecht Lauren (11), Miller Nicole (10), Sauder Paige (12), Stoughton Rebecca (10) | Peoria Notre Dame High School | Peoria | IL |