| Place | Time | Swimmers (Grades) | School | City | State |
| 1 | 1:41.63 | Arden Pitman (10), Alison Lusk (11), Anna Rae Gwarjanski (11), Sloane Pitman (12) | The Baylor School | Chattanooga | TN |
| 2 | 1:41.68 | Jessie Hammes (12), Lauren Stauder (11), Devon Mason (10), Megan Detro (12) | Carmel High School | Carmel | IN |
| 3 | 1:42.35 | Aubrey Peacock (11), Caitlin Mumford (12), Megan Fonteno (10), Dana Nessler (11) | The Bolles School | Jacksonville | FL |
| 4 | 1:42.99 | Chrissy Thomas (12), Kasey Carlson (11), Jaclyn Amog (12), Shaila Yoder (12) | Las Lomas High School | Walnut Creek | CA |
| 5 | 1:43.19 | Madeline Armitage (12), Nicole Wagner (11), Darcy Fishback (12), Kayla Hammerberg (10) | Upper Arlington High School | Upper Arlington | OH |
| 6 | 1:43.25 | Jillian Vitarius (9 ), Merritt Krawczyk (12), Dannye Osburn (12), Jamie Friderichs (9 ) | Kingwood High School | Kingwood | TX |
| 7 | 1:43.43 | Alexa Powers (12), Allison Gargalikis (10), Christina Ray (9 ), Deirdre Clute (11) | Carondelet High School | Concord | CA |
| 8 | 1:43.51 | Shirley Wang (10), Natalie Sanchez (10), Dayna Gettel (9 ), Marissa Konicke (10) | Plano Senior High School | Plano | TX |
| 9 | 1:43.60 | Cindy Tran (11), Sarah Moss (10), Ellie Morrissey (9 ), Kiersten Colesen (12) | Edison High School | Huntington Beach | CA |
| 10 | 1:43.75 | Bonnie Brandon (9 ), Christine Wixted (11), Kelly Naze (9 ), Katie Naze (11) | Cherry Creek High School | Greenwood Village | CO |
| 11 | 1:43.78 | Rachel Flinn (11), JiYoung Lee (12), Xiao Ying Zhao (12), Carolyn Fittin (10) | Peddie School | Hightstown | NJ |
| 12 | 1:43.98 | Sarah Lohman (9 ), Tori Bagan (10), McKenzie Bagan (12), Emily Keefer (12) | Homestead High School | Fort Wayne | IN |
| 13 | 1:44.06 | Lauren Driscoll (9 ), Melissa Cooper (11), Caroline Kuczynski (12), Courtney Randolph (11) | American Heritage School | Plantation | FL |
| 14 | 1:44.28 | Allie Roberts (10), Morgan Oxley (12), Deidre Geroni (12), Catherine Carr (11) | Emmaus High School | Emmaus | PA |
| 15 | 1:44.31 | Paige Devine (10), Sarah Liang (10), Margaret Wenzlau (9 ), Jasmine Tosky (9 ) | Palo Alto High School | Palo Alto | CA |
| 16 | 1:44.47 | Chelsea Griffiths (12), Jordan Danny (12), Maggie Hanson (11), Good Hayley (10) | William S. Hart High School | Newhall | CA |
| 17 | 1:44.68 | Lauren Damian (10), Paige Bradley (12), Callie Klein (10), Kelly Mendoza (11) | Monte Vista High School | Danville | CA |
| 18 | 1:44.74 | Samantha Wierzbicki (11), Kylie Reiter (9 ), Paige Impink (11), Taylor Straub (10) | Wilson High School | West Lawn | PA |
| 19 | 1:44.85 | Kara Savegnago (12), Elisabeth Tavierne (12), Powers MacKenzie (11), Hare Catherine (9 ) | Rosary High School | Aurora | IL |
| 20 | 1:44.93 | Vanessa Pollitt (12), Lauren Stoeckle (9 ), Alex Todd (10), Alyssa Swanson (12) | Regis Jesuit High School | Aurora | CO |
| 21 | 1:44.94 | Morgan Smith (12), Chelsea Leary (12), Casey Clark (10), Erica Dittmer (11) | Klein High School | Klein | TX |
| 22 | 1:45.05 | Jana Mangimelli (12), Anna Springer (11), Haley Holcombe (12), Meghan Faulkner (10) | Brookwood High School | Snellville | GA |
| 23 | 1:45.11 | Diandra Prutton (12), Hannah Curran (11), Rachel O'Brien (12), M.E. Randall (11) | College Park High School | Pleasant Hill | CA |
| 24 | 1:45.12 | Erin Reisinger (11), Aven Jackson (10), Mary Rose Palermo (11), Jessica Graber (11) | Walton High School | Marietta | GA |
| 25 | 1:45.17 | Sarah Andrews (12), Rachel Mays (9 ), Jackie Swartz (11), Molly Osterhage (11) | Centerville High School | Centerville | OH |
| 26 | 1:45.27 | Courtney Bartholomew (9 ), Emily Bos (11), Caroline Meyer (12), Nicole Cordero (9 ) | Holland High School | Holland | MI |
| 27 | 1:45.28 | Danielle Smith (11), Charlotte Williams (12), Fallon Fitzpatrick (11), Harper Bruens (9 ) | Boca Raton High School | Boca Raton | FL |
| 28 | 1:45.33 | Lauren Piper (11), Corinne Showalter (12), Rocky Raybon (11), Ashlee Linn (9 ) | Riverview High School | Sarasota | FL |
| 29 | 1:45.47 | Maeve McHugh (11), Rachel Dekar (12), Alexandria Miller (10), Taylor Abbott (12) | Cranbrook Kingswood High School | Bloomfield Hills | MI |
| 30 | 1:45.58 | Kim Pavlin (11), Ashley Blanchette (11), Aurelia Okeefe (10), Meghann McGoldrick (12) | Churchill High School | San Antonio | TX |
| 31 | 1:45.63 | Lauren Scott (12), Kristen Bates (11), Morgan Presley (9 ), Katie Haycraft (12) | Starr's Mill High School | Fayetteville | GA |
| 32 | 1:45.66 | Jennifer Reese (10), Chelsey Eberwein (12), Kelsey Amundsen (12), Mary Foster (12) | The Woodlands High School | The Woodlands | TX |
| 33 | 1:45.76 | Julia Carroll (11), Megan Sellers (9 ), Elena Carvell (12), Andrea Baumgartner (11) | Waubonsie Valley High School | Aurora | IL |
| 34 | 1:45.78 | Stephanie Wei (12), Eva Chen (10), Charlotte Ruby (9 ), Laurence Delisle (12) | Archbishop Mitty High School | San Jose | CA |
| 34 | 1:45.78 | Kate Jones (9 ), Caryn Knight (11), Emma Goral (10), Holly Johnson (10) | Arrowhead High School | Hartland | WI |
| 36 | 1:45.83 | Marisa McGlynn (11), Miranda Mercke (9 ), Summer Stephens (10), Clark Cromer (11) | Sacred Heart Academy | Louisville | KY |
| 37 | 1:45.87 | Molly Albrecht (12), Reilly Moore (10), Chloe Portela (10), Morgan Flannigan (11) | Lawrence Free State High School | Lawrence | KS |
| 38 | 1:45.95 | Maegan O'Connor (12), Erin Kelley (12), Paige Copeland (11), Aviry Reich (11) | Hamilton Southeastern High School | Fishers | IN |
| 39 | 1:46.00 | Taylor Sneed (10), Madeline Maher (9 ), Bridget Casey (10), Rebecca Dugan (10) | Germantown Academy | Ft. Washington | PA |
| 40 | 1:46.02 | Jackie Powell (11), Kendra Loch (12), Kelsie Saxe (12), Kayla Smith (12) | Madison Memorial High School | Madison | WI |
| 41 | 1:46.17 | Yurie Nakano (10), Samantha Rapp (11), Jordan Liedholm (12), Katelyn Miller (10) | Hershey High School | Hershey | PA |
| 42 | 1:46.41 | Katie Bootsma (10), Stephanie Solfelt (12), Rachel Bootsma (9 ), Claire Ritzen (12) | Eden Prairie High School | Eden Prairie | MN |
| 42 | 1:46.41 | Courtney Ciesielski (11), Shannon O'Malley (10), Lindzi Smith (12), Kim Carducci (10) | Lassiter High School | Marietta | GA |
| 44 | 1:46.48 | Kelsey Ferrante (10), Colleen Sheldon (12), Jackie Rzymek (12), Sarah Anderson (12) | McDowell High School | Erie | PA |
| 45 | 1:46.49 | Corinne Talhouk (10), Kylie Grady (11), Courtney Thompson (11), Allie Pennetti (9 ) | North Allegheny High School | Wexford | PA |
| 46 | 1:46.50 | Ruby Martin (12), Aja Van Hout (10), Becca Soderholm (11), Ivy Martin (10) | Madison East High School | Madison | WI |
| 47 | 1:46.51 | Leslie Hackler (12), Julia Treible (11), Kristen Redmond (12), Elizabeth Saint (10) | Centennial High School | Roswell | GA |
| 48 | 1:46.56 | Libby Hunsche (11), Gabbie Pettinichi (11), Brittany Groene (12), Valerie Borger (9 ) | Turpin High School | Cincinnati | OH |
| 49 | 1:46.68 | Jane Munro (11), Roxy Capron (11), Kristina Walsh (11), Maddie Giegold (12) | Evanston Township High School | Evanston | IL |
| 49 | 1:46.68 | Taylor Steffl (10), Alexandra Swies (12), Maria Bargardi (10), Kaitlyn Jefferson (12) | Mercy High School | Farmington Hills | MI |
| 51 | 1:46.69 | Kearsten Livingstone (11), Sarah Pino (12), Kirsten Justice (9 ), Allison Morales (10) | Canyon High School | Canyon Country | CA |
| 52 | 1:46.74 | Sara Snow (11), Deanna Sorenson (12), Elizabeth Mace (11), Olivia Harrell (11) | Glendale High School | Springfield | MO |
| 53 | 1:46.79 | Audrey Welklin (11), Mackenzie Imbro (12), Nora Brolsma (12), Claire Brolsma (9 ) | Noblesville High School | Noblesville | IN |
| 53 | 1:46.79 | Shannon Landgrebe (12), Elle Wohlmuth (11), Emily Andelson (12), Katie Pawelczyk (12) | Palm Desert High School | Palm Desert | CA |
| 55 | 1:46.80 | Lauren Gehrke (11), Ellyn Baumgardner (12), Jean DeOrnellas (12), Theresa Harvey (12) | Fairfax High School | Fairfax | VA |
| 55 | 1:46.80 | Hannah Messer (11), Katie McCullough (12), Marissa Murphy (11), Taylor Folkertsma (10) | Forest Hills Central High School | Grand Rapids | MI |
| 57 | 1:46.90 | Courtney Raw (12), Anna Marshall (9 ), Megan Lydzinski (9 ), Gwen Comollo (12) | Montgomery High School | Skillman | NJ |
| 58 | 1:46.92 | Kelsea Cross (12), Myra Retrum (9 ), Siri Retrum (11), Lauren Gustafson (12) | Columbus North High School | Columbus | IN |
| 59 | 1:46.96 | Brittany Fozkos (11), Olivia Kabacinski (9 ), Meghan McLaughlin (10), Mallory Przybylinski (12) | Chesterton High School | Chesterton | IN |
| 60 | 1:47.04 | Erika Elliot (9 ), Emily Fogle (11), Anne Jacobsen (10), Kimmy Steelman (12) | Barrington High School | Barrington | IL |
| 61 | 1:47.07 | Cami Hansberry (12), Marley Suckow (11), Natalie Morris (10), Maddy Dalcamo (11) | Olathe Northwest High School | Olathe | KS |
| 62 | 1:47.10 | Grace Tarke (12), Claire Collins (11), Megan Leung (11), Elizabeth Rosen (9 ) | St. Ignatius College Preparatory | San Francisco | CA |
| 63 | 1:47.18 | Brooke Waugaman (10), Jami Andrews (12), Morgan Stoner (10), Olivia Forish (12) | Hempfield Area School District | Greensburg | PA |
| 64 | 1:47.21 | Shelby Williams (9 ), Lauren Norberg (12), Lindsey Norberg (12), Nicole Heise (12) | Ithaca High School | Ithaca | NY |
| 65 | 1:47.22 | Caitlyn Forman (9 ), Ellen Williamson (10), Audrey Lawson (12), Tully Bradford (11) | Notre Dame Academy | Park Hills | KY |
| 66 | 1:47.29 | Taylor Munk (11), Elizabeth Parrish (12), Marissa Neel (9 ), Keri George (12) | San Ramon Valley High School | Danville | CA |
| 67 | 1:47.30 | Laura Evans (12), Emily Weir (11), Morgan Karetnick (9 ), Shannon Jones (11) | Greenwich High School | Greenwich | CT |
| 67 | 1:47.30 | Katie Duggan (10), Zoe Sianis (12), Brianna Bentz (10), Mary Moser (11) | Wyomissing Area High School | Wyomissing | PA |
| 69 | 1:47.35 | Kyra Lindholm (10), Afton Robertson (12), Autumn Baldwin (11), Alexis Fee (11) | Marian High School | Omaha | NE |
| 70 | 1:47.36 | Gigi Groya (10), Madeleine Moeller (10), Meg Vertovec (12), Sarah Norris (12) | Lyons Township High School | La Grange | IL |
| 70 | 1:47.36 | Kellie Barbiea (10), Olivia Mapes (12), Dani Barbiea (10), Devin Spencer (11) | Springboro High School | Springboro | OH |
| 72 | 1:47.39 | Krista Johnson (12), Ali Tomkinson (9 ), Monica Dornick (10), katie Tomkinson (12) | Carlsbad High School | Carlsbad | CA |
| 73 | 1:47.41 | Hailey Campbell (11), Melissa McMahon (11), Natalie Herrild (12), Wendy Consoer (12) | Lakeville South High School | Lakeville | MN |
| 74 | 1:47.51 | Courtney Grace (10), Melanie Margalis (11), Paige Schmidt (12), Rachel Burns (12) | Countryside High School | Clearwater | FL |
| 75 | 1:47.52 | Ellen Illg (12), Karen Illg (10), Sshannon Guy (11), Anna Howard (12) | Millard West High School | Omaha | NE |
| 76 | 1:47.53 | Cori Michibata (9 ), Rebecca Lewinson (11), Jocelyn Yuen (9 ), Brynja Winnan (11) | West Windsor- Plainsboro South High School | Princeton Junction | NJ |
| 77 | 1:47.55 | Danielle Harris (12), Imelda Wistey (11), Nailah Bakari (10), Kelsey Galles (11) | Valley High School | West Des Moines | IA |
| 78 | 1:47.57 | Taylor Kitayama (11), Tara DeMuth (12), Lauren Rigg (11), Rachael Cazden (12) | Fairview High School | Boulder | CO |
| 78 | 1:47.57 | Morgan Cohara (9 ), Brittany Rospierski (12), Alana Arnold (10), Dana Shaker (11) | Hawken School | Gates Mills | OH |
| 80 | 1:47.60 | Ashley Cohagen (12), Valerie Barthelemy (12), Molly Shanley (10), Laura Cameron (12) | Ann Arbor Pioneer High School | Ann Arbor | MI |
| 81 | 1:47.65 | Laurel Chaney (12), Kennedy Higdon (11), Hannah Pinion (10), Meg McWhorter (11) | Bearden High School | Knoxville | TN |
| 81 | 1:47.65 | Hayley Russell (11), Olivia Alva (12), Caroline Lukins (12), Dana Holt (10) | Campolindo High School | Moraga | CA |
| 81 | 1:47.65 | Tess Behrens (11), Brooke Robbins (12), Danielle Adank (12), Ellen Dobbs (11) | Maple Grove Senior High | Maple Grove | MN |
| 84 | 1:47.67 | Taylor Derevyanik (9 ), Chelsea Dunlap (11), Rachael Ausdenmoore (12), Laura Huning (12) | Southlake Carroll High School | Southlake | TX |
| 85 | 1:47.68 | Katie Anderson (9 ), Danielle Ortizharo (12), Kathryn Bowersox (9 ), Danielle Smyth (12) | Rancho Bernardo High School | San Diego | CA |
| 86 | 1:47.76 | Erin Gaeckle (10), Christen McDonough (11), Dana Pruitt (11), Mary Kate Hetzel (11) | Charlotte Catholic | Charlotte | NC |
| 87 | 1:47.77 | Sarah Galvin (12), Caitlin Pilgrim (9 ), Catherine Cortesio (11), Allison Pavlik (9 ) | Benet Academy | Lisle | IL |
| 87 | 1:47.77 | Kristine Polley (12), Kim Zachensky (12), Liz Braun (11), Lindsey Jackson (12) | Sprayberry High School | Marietta | GA |
| 89 | 1:47.79 | Kristin Gerkens (12), Grace Hoffman (11), Gemma Hoffman (11), Annie Yin (11) | Allen D. Nease High School | Ponte Vedra | FL |
| 89 | 1:47.79 | Amy Katz (10), Alexandra Isaacs (12), Alex Hoffman (10), Jennifer Miles (12) | North Central High School | Indianapolis | IN |
| 91 | 1:47.82 | Rebecca Reed (12), Romy Landeck (9 ), Olivia Lin (12), Danielle Yoho (12) | Clements High School | Sugar Land | TX |
| 91 | 1:47.82 | Lauren Rosenberg (11), Ashley Specht (12), Alyssa Heydorn (11), Rachel Chickerella (12) | Olentangy Liberty High School | Powell | OH |
| 93 | 1:47.83 | Lauren Davis (10), Megan Raitt (12), Tiffany Sudarma (9 ), Michelle Huang (11) | Westwood High School | Austin | TX |
| 94 | 1:47.84 | Nina Damjanovich (11), Stacey Wierman (11), Brittany Gilbert (9 ), Jillian Homans (9 ) | Munster High School | Munster | IN |
| 95 | 1:47.90 | Riley Flanagan (11), Alison Daly (12), Hollie Harrison (12), Emily Brown (9 ) | Buchholz High School | Gainesville | FL |
| 96 | 1:47.91 | Hayley Johnson (10), Claire Schelske (10), Jaynie Pulte (9 ), Stephanie Berg (11) | Marian High School | Bloomfiled Hills | MI |
| 97 | 1:47.92 | Jesse Bergman (11), Anne Peanasky (11), Lisa Yao (12), Kristen Clarno (9 ) | Homestead High School | Cupertino | CA |
| 98 | 1:47.97 | Shaya Schaedler (9 ), Meghan Swirzcki (12), TJ Padova (12), Brenda Bishop (10) | Campbell County High School | Gillette | WY |
| 99 | 1:47.98 | Gretchen Hestley (12), Brianna Sciara (9 ), Maryn Bieganski (11), Emily Schwartz (11) | Solon High School | Solon | OH |
| 100 | 1:48.02 | Eleanor Prewitt-Thomas (12), Krist Fries (12), Ibby Simcox (9 ), Carina Kirchner (11) | Blue Springs High School | Blue Springs | MO |
| 100 | 1:48.02 | Amber McDermott (10), Marina Pomar-Enders (12), Alana Pazevic (11), Erin Holliday (12) | H. M. Jackson High School | Mill Creek | WA |
| 102 | 1:48.07 | Ashley Brewer (11), Kasey Taylor (11), Malia Johnson (11), Carly Johnson (9 ) | Chaparral High School | Scottsdale | AZ |
| 102 | 1:48.07 | Lauren Opatrny (11), Natalie Cheung (9 ), Elizabeth Murray (11), Megan Nolet (11) | Saratoga High School | Saratoga | CA |
| 104 | 1:48.08 | Ashley Henderson (9 ), Melissa Andruzzi (12), Brittany Wallace (10), Jessica Andruzzi (10) | The Latin School | Chicago | IL |
| 105 | 1:48.12 | Julianne Steege (12), Olivia McNeely (9 ), Maddy DeMeules (11), Sophie Pilhofer (12) | Visitation High School | Mendota Heights | MN |
| 106 | 1:48.15 | Da Yae Choi (9 ), Genevieve Bradford (9 ), Aya Fujimura (11), Caitlin Lipton (12) | Woodbridge High School | Irvine | CA |
| 107 | 1:48.16 | Meredith Sweeney (11), Sarah Coady (12), Genna Heidkamp (9 ), Sara Shaner (11) | Hinsdale Central High School | Hinsdale | IL |
| 107 | 1:48.16 | Ruthie Norval (11), Camille Hendrix (12), Sarah Southworth (11), Katelin Scarsella (12) | Westlake High School | Austin | TX |
| 109 | 1:48.21 | Hannah Cooper (10), Catherine Wagner (11), Kristin Rollins (12), Zoe Bond (10) | Memorial High School | Houston | TX |
| 110 | 1:48.22 | Keeley Chenet (12), Emily Schon (11), Courtney Chenet (11), Anna Connolly (12) | Conestoga High School | Berwyn | PA |
| 111 | 1:48.26 | Lauren Hines (12), Katie Young (9 ), Drew Dillon (11), Devin Henry (11) | Hidden Valley High School | Roanoke | VA |
| 112 | 1:48.30 | Anna DeMonte (9 ), Katie Chamberlain (12), Elizabeth Gelb (10), Annie Tamblyn (9 ) | Ann Arbor Huron High School | Ann Arbor | MI |
| 113 | 1:48.31 | Lauren Reynolds (9 ), Angie Chokran (11), Taylor Gannon (11), Allie Smith (9 ) | St. Charles North High School | St. Charles | IL |
| 114 | 1:48.32 | Maggi Beck (12), Katelyn Webster (10), Dagny Knutson (11), Courtney Funk (9 ) | Minot High School | Minot | ND |
| 115 | 1:48.33 | Emily WIlkinson (11), Adrienne Bicek (12), Haley Sims (10), Caitlin Landry (11) | Downers Grove North High School | Downers Grove | IL |
| 116 | 1:48.35 | Marina Spadoni (9 ), Camille Hopp (12), Ashlyn Acosta (11), Nicole Skaggs (9 ) | Mission Viejo High School | Mission Viejo | CA |
| 117 | 1:48.36 | Melissa Cropper (11), Kim Weyand (10), Sarah Lloyd (11), Emily Lloyd (11) | Severna Park High School | Severna Park | MD |
| 117 | 1:48.36 | Ellena Basada (9 ), Kara Stranski (11), Mary Byers (12), Kayla Alf-Huynh (12) | St. Mary's Academy | Portland | OR |
| 119 | 1:48.39 | Kyra Jones (11), Charlotte Lechner (9 ), Anne Steele (10), Bri Dillon (10) | Seven Lakes High School | Katy | TX |
| 120 | 1:48.41 | Kyra Logan (10), Jacqueline Painter (12), Christina Bechtel (10), Alia Welsh (10) | Franklin High School | Franklin | TN |
| 120 | 1:48.41 | Victoria Kuhn (9 ), Reia Tong (9 ), Danielle Schulkin (11), Lauren Poore (11) | Walt Whitman High School | Bethesda | MD |
| 122 | 1:48.42 | Haley Smith (12), Ana Kuehler (12), Katie Kruse (10), Kelsey Bakalar (12) | Blue Valley North High School | Overland Park | KS |
| 123 | 1:48.43 | Madeleine Klichowski (10), Michelle Garland (9 ), Mallory Remick (9 ), Ellie Ansani (10) | Loyola Academy | Wilmette | IL |
| 123 | 1:48.43 | Blair Moody (12), Katie Edwards (12), Claudia Ruiz (12), Pauline Stewart (12) | Rio Americano High School | Sacramento | CA |
| 125 | 1:48.45 | Addison Koelle (12), Adelaide Taylor (11), Kaley Kokomoor (12), Alivia Berg (11) | Fitch Senior High School | Groton | CT |
| 125 | 1:48.45 | Rachel Roman (11), Mackenzie Hoffman (12), Beth DeFauw (11), Molly Buynak (12) | Strongsville High School | Strongsville | OH |
| 127 | 1:48.48 | Meghan Talbot (10), Mady Gockowski (9 ), Kaela Anderson (11), Monica Radecke (11) | Stillwater Area High School | Stillwater | MN |
| 128 | 1:48.53 | Shannon Adkins (12), Haley Anderson (12), Shelby Romuk (11), Quincy Christian (11) | Granite Bay High School | Granite Bay | CA |
| 129 | 1:48.55 | Kirsten Milberg (12), Londyn Clawson (12), Taylor Curado (12), Jehan Atoe (12) | Rock Canyon High School | Highlands Ranch | CO |
| 130 | 1:48.58 | Elle Meinholz (12), Anna Meinholz (9 ), Ashley Majewski (12), Lauren Schoendorf (12) | Catholic Memorial High School | Waukesha | WI |
| 131 | 1:48.60 | Mary Wunderle (12), Haley Bull (12), Katie Heywood (10), Danielle Jansen (11) | Mason High School | Mason | OH |
| 132 | 1:48.61 | Kimmy Ballo (12), Tera Bradham (10), Bryn Lewis (12), Jessica Leger (12) | Round Rock High School | Round Rock | TX |
| 132 | 1:48.61 | Jenn Abbott (11), Kaylin Moss (9 ), Kelly Heyde (12), Caroline Higgins (11) | Wilton High School | Wilton | CT |
| 134 | 1:48.64 | Kathleen VanDeVoorde (11), Jennifer Strom (10), Claire Anderson (10), Gretchen Heidorn (11) | Rochester Century High School | Rochester | MN |
| 135 | 1:48.66 | Gretchen Jaques (10), Courtney McCadden (10), Kelly Corso (10), Rosemary Garcia (9 ) | Glendora High School | Glendora | CA |
| 136 | 1:48.68 | Taylor Newgaard (11), Katie Olsen (10), Lauren Torres (10), Tinsley Andrews (11) | Desert Vista High School | Phoenix | AZ |
| 136 | 1:48.68 | Tanner Harris (9 ), Allison Arnold (11), Hannah Ferrin (12), Katie Streepey (11) | Highland Park High School | Dallas | TX |
| 138 | 1:48.76 | Kathleen Durkan (11), Kelly Carroll (10), Lauren Keenan (10), Catherine Shaughnessy (9 ) | Great Valley High School | Malvern | PA |
| 138 | 1:48.76 | Annie MacKay (10), Kayla Lehman (9 ), Rachael Dzierzak (9 ), Ellen Dzierzak (11) | Toledo Notre Dame Academy | Toledo | OH |
| 140 | 1:48.82 | Courtney Busch (11), Emily Wiltsie (11), Shea Brunsvold (12), Ali Stearns (10) | Mason City High School | Mason City | IA |
| 140 | 1:48.82 | Kelse Johnson (9 ), Caroline Piehl (10), Rachel Szado (11), Courtney Wedel (10) | Smoky Hill High School | Aurora | CO |
| 142 | 1:48.83 | Laura Finelli (11), Rachel Canty (9 ), Madison Rupp (12), Amanda Kendall (12) | Robinson Secondary School | Fairfax | VA |
| 143 | 1:48.87 | Rachel Curtiss (11), Megan Goss (11), Kimberly Davis (10), Stephanie Pringle (11) | Ravenwood High School | Brentwood | TN |
| 144 | 1:48.88 | Maya DiRado (11), Karissa DeRousseau (10), Lauren Matevish (11), Neda Kazemini (12) | Maria Carrillo High School | Santa Rosa | CA |
| 144 | 1:48.88 | Alex LeForge (12), Kaylin Burchell (10), Kelcy Perry (12), Meredith White (9 ) | Model Laboratory School | Richmond | KY |
| 146 | 1:48.89 | Anna Labozan (10), Whitney Fletcher (12), Whitney Dougherty (11), Melissa Tillman (11) | Monarch High School | Louisville | CO |
| 147 | 1:48.90 | Heather O'Toole (12), Emily Kintz (12), Chandler Ryberg (9 ), Haley Durmer (9 ) | St. Pius X Catholic High School | Atlanta | GA |
| 148 | 1:48.92 | Nadia El-Hamdi (10), Koryn Ternes (9 ), Erin Flanigan (10), Jessica Gardner (11) | James Bowie High School | Austin | TX |
| 148 | 1:48.92 | Dorothy Yu (12), Cathy Chen (11), Maria Watkins (11), Yvonne Huo (10) | Winston Churchill High School | Potomac | MD |
| 150 | 1:48.95 | Samantha James (9 ), Caroline Conklin (10), Kathryn Lee (12), Megan Marchuk (10) | New Trier High School | Winnetka | IL |
| 151 | 1:48.96 | Ashley Mitson (10), Annie Lazor (9 ), Molly Tyler (12), Elise Dalley (10) | Birmingham Groves High School | Beverly Hills | MI |
| 152 | 1:48.98 | Kaitlyn Lo (11), Liliana Alvarez (11), Julia Alvarez (11), Bailey Kinsolving (9 ) | Sir Francis Drake High School | San Anselmo | CA |
| 152 | 1:48.98 | Jessica Swanson (12), Natalie Lugg (11), Samantha McNeil (12), Britta Sortland (10) | Woodbury High School | Woodbury | MN |
| 154 | 1:49.02 | Bethany DeWitt (9 ), Laura McGlaughlin (12), Megan Corcoran (11), Julie Lin (12) | Bayard Rustin High School | West Chester | PA |
| 155 | 1:49.04 | Alison Meng (10), Carly Reinke (9 ), Kelsey Kohlbeck (10), Abby Diehl (12) | Sauk Prairie High School | Prairie du Sac | WI |
| 156 | 1:49.09 | Molly Raspopovich (12), Hannah Raspopovich (9 ), Haley Balko (9 ), Ashley Gose (11) | Crown Point High School | Crown Point | IN |
| 157 | 1:49.10 | Elizabeth Floyd (12), Stephanie Ferrell (10), Janene Senofonte (12), Kristen Moll (12) | Mount Saint Mary Academy | Watchung | NJ |
| 158 | 1:49.16 | Sana Alloo (11), Natalie Leake (11), Taylor Avery (10), Susie Lesniewski (11) | Centreville High School | Clifton | VA |
| 159 | 1:49.18 | Kellie Darmody (12), Susie Raichle (10), Anne Safran (12), Bailey Woodling (11) | Providence High School | Charlotte | NC |
| 159 | 1:49.18 | Isabela Castro (12), Kylie Harper (12), Meagan Stone (12), Julia Moraes (11) | Timpview High School | Provo | UT |
| 161 | 1:49.21 | Andie W. Tibbetts (11), Erin E. Brady (11), Christiana N. Thew (11), Emma L. Nuzzo (9 ) | Phillips Exeter Academy | Exeter | NH |
| 162 | 1:49.24 | Mary Lacine (11), Emma Newman (10), Tatum Stewart (12), Mamie Hobbs (12) | Champaign Central High School | Champaign | IL |
| 163 | 1:49.25 | Ashley Bush (12), Lauren Kalinosky (12), Kerry Abel (12), Rebecca Berman (10) | Novi High School | Novi | MI |
| 164 | 1:49.26 | Kayley Dodson (11), Jessica Mosbaugh (9 ), Stephanie Schuffles (9 ), Darielle Watkins (10) | Los Alamitos High School | Los Alamitos | CA |
| 165 | 1:49.29 | Casey Dollard (11), Jacqueline Woods (9 ), Morgan Bonk (12), Stephanie Ovalle (11) | Fenwick High School | Oak Park | IL |