| Place | Time | Relay | School | City | State |
| 1 | 3:23.53 | Chelsea Fong (11), Megan Fischer-Colbrie(12), Audrey Kwong (12), Emma Drysdale (12) | Monta Vista High School | Cupertino | CA |
| 2 | 3:23.72 | Katelin Scarsella (12), Camille Hendrix (12), Ruthie Norval (11), Samantha Tucker (11) | Westlake High School | Austin | TX |
| 3 | 3:23.97 | Aubrey Peacock (11), Dana Nessler (11), Casey Sandlin (12), Megan Fonteno (11) | The Bolles School | Jacksonville | FL |
| 4 | 3:24.20 | Kim Pavlin (11), Becca Ploetz (12), Meghann McGoldrick (12), Lily Moldenhauer (11) | Churchill High School | San Antonio | TX |
| 5 | 3:24.22 | Carly Marshall (10), Rhiannon Sheets (10), Logan Mason (12), Trish Regan (12) | Carmel High School | Carmel | IN |
| 6 | 3:24.80 | Aja VanHout (10), Ivy Martin (10), Becca Soderholm (11), Ruby Martin (12) | Madison East High School | Madison | WI |
| 7 | 3:25.29 | Maegan O'Connor (12), Aviry Reich (11), Aubrey Hertzler (11), Carly Mercer (11) | Hamilton Southeastern High School | Fishers | IN |
| 8 | 3:25.30 | Missy Doll (11), Paige Whitmire (12), Chloe Thomas (10), Angela Severn (12) | Souderton Area High School | Souderton | PA |
| 9 | 3:25.37 | Lauren McCandless (11), Allie Roberts (10), Alena Kluge (11), Mikelle Masciantonio (11) | Emmaus High School | Emmaus | PA |
| 10 | 3:25.56 | Erin Foster (10), Genevieve Swartz (12), Erika Deardorf (12), Mary Foster (12) | The Woodlands High School | The Woodlands | TX |
| 11 | 3:25.69 | Carolyn Fittin (10), JiYoung Lee (12), Kathryn Raplee (12), Laura Loughran (11) | Peddie School | Hightstown | NJ |
| 12 | 3:25.85 | Kristyn Fulcher (10), Jennifer Warmolts (12), Allison Kerry Sullivan (12), Abigail Chin (10) | Upper Arlington High School | Upper Arlington | OH |
| 13 | 3:25.87 | Stephanie Eisenring (12), Lara Moseley (12), Siena DeVenuto (11), Rebecca Aiello (10) | Pine Crest School | Fort Lauderdale | FL |
| 14 | 3:26.05 | Jasmine Tosky (9 ), Sabrina Lee (10), Allie Bollella (12), Abby Duckett (9 ) | Palo Alto High School | Palo Alto | CA |
| 15 | 3:26.09 | Kaela Anderson (11), Jordan Bowen (12), Betsy Galenti (12), Hannah Bowen (10) | Stillwater High School | Stillwater | MN |
| 16 | 3:26.23 | Kara Savegnago (12), Olivia Scott (11), Fayhee Kally (12), Rachel Burke (9 ) | Rosary High School | Aurora | IL |
| 17 | 3:26.54 | Allyson Sweeney (12), Kelsey Gouge (9 ), Meghan Faulkner (10), Jana Mangimelli (12) | Brookwood High School | Snellville | GA |
| 18 | 3:26.61 | Jordan Mattern (10), Kelly Naze (9 ), Loren Brandon (12), Bonnie Brandon (9 ) | Cherry Creek High School | Greenwood Village | CO |
| 19 | 3:26.62 | Maddy Urban (11), Megan Marchuk (10), Samantha James (9 ), Kate Shumway (12) | New Trier High School | Winnetka | IL |
| 20 | 3:27.10 | Stephanie Wei (12), Charlotte Ruby (9 ), Monica Garcia (11), Delisle Laurence (12) | Archbishop Mitty High School | San Jose | CA |
| 21 | 3:27.17 | Katie Nolan (12), Alexis Grier (9 ), Fiona Mancuso (11), Jordan Liedholm (12) | Hershey High School | Hershey | PA |
| 22 | 3:27.38 | Sydney Schaefer (12), Meredith Sweeney (11), Caitlin Connor (12), Sara Shaner (11) | Hinsdale Central High School | Hinsdale | IL |
| 23 | 3:27.52 | Paige Gilley (11), Natalie Naruns (12), Jacqueline Levere (10), Maddy Schaefer (10) | Saint Francis High School | Mountain View | CA |
| 24 | 3:27.58 | Sloane Pitman (12), Emma Michaels (10), Anna Rae Gwarjanski (11), Arden Pitman (10) | The Baylor School | Chattanooga | TN |
| 25 | 3:28.18 | Alli Gillespie (10), Ellie Morrissey (9 ), Danielle Warde (11), Cindy Tran (11) | Edison High School | Huntington Beach | CA |
| 26 | 3:28.22 | Courtney Bartholomew (9 ), Haley Thompson (9 ), Klare Northuie (9 ), Emily Bos (11) | Holland High School | Holland | MI |
| 26 | 3:28.22 | Jackie Powell (11), Kelsie Saxe (12), Mackenzie King (12), Kayla Smith (12) | Madison Memorial High School | Madison | WI |
| 28 | 3:28.34 | Morgan Smith (12), Chelsea Leary (12), Casey Clark (10), Erica Dittmer (11) | Klein High School | Klein | TX |
| 29 | 3:28.46 | Caitlyn Karr (10), Camilla Czulada (9 ), Taylor Brew (9 ), Paige Impink (11) | Wilson High School | West Lawn | PA |
| 30 | 3:28.47 | Emily Boone (10), Catie Clark (10), Camey Rabold (9 ), Gracie Finnegan (11) | Bishop Watterson High School | Columbus | OH |
| 30 | 3:28.47 | Shannon Landgrebe (12), Elle Wohlmuth (11), Katie Pawelczyk (12), Emily Andelson (12) | Palm Desert High School | Palm Desert | CA |
| 32 | 3:28.65 | Laura Slater (11), Kristina Parsons (12), Deborah Roth (11), Azita Sayadi (11) | Redwood High School | Larkspur | CA |
| 33 | 3:29.01 | Molly Osterhage (11), Katie Czachor (9 ), Jackie Swartz (11), Sarah Andrews (12) | Centerville High School | Centerville | OH |
| 34 | 3:29.05 | Maggie Hanson (11), Chelsea Griffiths (12), Jordan Danny (12), Asencio Samantha (10) | William S. Hart High School | Newhall | CA |
| 35 | 3:29.20 | Kaitlyn Jefferson (12), Megan Gegesky (12), Maria Bargardi (10), Nikki Barczak (10) | Mercy High School | Farmington Hills | MI |
| 36 | 3:29.33 | Riley Flanagan (11), Hollie Harrison (12), Hillary Petersen (11), Jamie Bohunicky (12) | Buchholz High School | Gainesville | FL |
| 37 | 3:29.34 | Jillian Garton (12), Shannon Adkins (12), Sophie Weber (9 ), Haley Anderson (12) | Granite Bay High School | Granite Bay | CA |
| 38 | 3:29.42 | Morgan Emter (9 ), Cari Caprio (11), Mary Wunderle (12), Danielle Jansen (11) | Mason High School | Mason | OH |
| 39 | 3:29.49 | Ellie McGinn (10), Anna Howard (12), Bianca Christensen (12), Emma O'Connell (10) | Millard West High School | Omaha | NE |
| 40 | 3:29.76 | Shelby Webber (10), Alex Huynen (11), Danica Fascella (11), Cynthia Fascella (11) | Fountain Valley High School | Fountain Valley | CA |
| 41 | 3:29.78 | Ashley Brewer (11), Malia Johnson (11), Megan Cox (9 ), Leticia Lelli (9 ) | Chaparral High School | Scottsdale | AZ |
| 42 | 3:29.81 | Paige Bradley (12), Sloan Saunders (11), Ocean Trail (12), Sydney Saunders (11) | Monte Vista High School | Danville | CA |
| 43 | 3:29.85 | Cimone Miner (12), Elizabeth Keenan (11), Natalia Anchustegui (9 ), Haley Krakoski (11) | Xavier College Preparatory | Phoenix | AZ |
| 44 | 3:29.95 | Kim Summers (12), Merritt Krawczyk (12), Jillian Vitarius (9 ), Michelle Gean (11) | Kingwood High School | Kingwood | TX |
| 45 | 3:29.99 | Nicole Heise (12), Kailey Gardner (12), Lauren Norberg (12), Lindsey Norberg (12) | Ithaca High School | Ithaca | NY |
| 46 | 3:30.03 | Ashlee Linn (9 ), Cha Cha Bugatti (10), Corinne Showalter (12), Jessica Piper (9 ) | Riverview High School | Sarasota | FL |
| 47 | 3:30.04 | Kasey Carlson (11), Jaclyn Amog (12), Chrissy Thomas (12), Shaila Yoder (12) | Las Lomas High School | Walnut Creek | CA |
| 48 | 3:30.06 | Rachel Cleak (11), Andrea Ward (11), Courtney Whyte (10), Shelbi Luchini (12) | Campolindo High School | Moraga | CA |
| 49 | 3:30.16 | Kiera Janzen (10), Nicole Hutchins (9 ), Samantha Fox (10), Lauren Weis (12) | Cretin-Derham Hall High School | St. Paul | MN |
| 50 | 3:30.19 | Gwen Comollo (12), Kara Dubiel (12), Courtney Raw (12), Megan Lydzinski (9 ) | Montgomery High School | Skillman | NJ |
| 51 | 3:30.24 | Arianna Gorenc (12), Courtney Sheehan (10), Elise Borja (11), Pamela Breitrick (10) | Waukesha South High School | Waukesha | WI |
| 52 | 3:30.29 | Haley Pietila (9 ), Tracy Vogel (11), Emma Goral (10), Kate Jones (9 ) | Arrowhead High School | Hartland | WI |
| 53 | 3:30.32 | Kelly Grace (10), Melanie Margalis (11), Paige Schmidt (12), Rachel Burns (12) | Countryside High School | Clearwater | FL |
| 54 | 3:30.33 | Alex Bilunas (10), Meredith Colombo (11), Brianna Bilunas (10), Anna Kokensparger (12) | Duchesne Academy | Omaha | NE |
| 55 | 3:30.49 | Ashleigh Grammar (11), Samantha Wright (11), Jessi Holz (11), Shannon McCoy (11) | Lafayette High School | Wildwood | MO |
| 56 | 3:30.61 | Shauna Foor (11), Meaghan McLaughlin (10), Maddie Miller (9 ), Olivia Kabacinski (9 ) | Chesterton High School | Chesterton | IN |
| 56 | 3:30.61 | Claire Martin (12), Mariah Tharp (11), Mackenzie Schuler (10), Tristin Baxter (11) | Clovis West High School | Fresno | CA |
| 56 | 3:30.61 | Courtney Hubbard (12), Meagan Hairston (12), Olivia Arnold (12), Maureen McLaine (12) | Plano West Senior High School | Plano | TX |
| 59 | 3:30.69 | Hillary Thomas (10), Alyssa Swanson (12), Alex Todd (10), Vanessa Pollitt (12) | Regis Jesuit High School | Aurora | CO |
| 60 | 3:30.74 | Courtney Ciesielski (11), Ansley Crenshaw (11), Sarah Williford (9 ), Samantha Bosma (10) | Lassiter High School | Marietta | GA |
| 61 | 3:30.76 | Alexandra Whitford (11), Talor Tadena (11), Emily Rodgers (11), Angelina Pratt (11) | Bella Vista High School | Fair Oaks | CA |
| 62 | 3:30.78 | Leslie Hackler (12), Caitlin Bell (9 ), Julia Treible (11), Kristen Redmond (12) | Centennial High School | Roswell | GA |
| 63 | 3:30.90 | Katherine Daniels (10), Meredith Nakano (10), Elizabeth Pitts (9 ), Lauren Pfohl (12) | Marist School | Atlanta | GA |
| 64 | 3:31.05 | Rebecca Dugan (10), Macie Ciampoli (10), Bridget Casey (10), Sarah Aemisegger (10) | Germantown Academy | Ft. Washington | PA |
| 65 | 3:31.07 | Genevieve Bradford (9 ), Aya Fujimura (11), DaYae Choi (9 ), Caitlin Lipton (12) | Woodbridge High School | Irvine | CA |
| 66 | 3:31.10 | Adrienne Bicek (12), Jessica Johnson (12), Emma Buckley (11), Haley Sims (10) | Downers Grove North High School | Downers Grove | IL |
| 67 | 3:31.13 | Kristina Jaeger (12), Paige Kostishak (10), Ashley Cohagen (12), Hanna Cowley (10) | Ann Arbor Pioneer High School | Ann Arbor | MI |
| 68 | 3:31.15 | Annie Marquiss (10), Emily Schwartz (11), Melissa Besman (11), Gretchen Hestley (12) | Solon High School | Solon | OH |
| 69 | 3:31.17 | Jacqueline Ring (12), Colleen Williams (9 ), Fallon Fitzpatrick (11), Danielle Smith (11) | Boca Raton High School | Boca Raton | FL |
| 70 | 3:31.22 | Jackie Rzymek (12), Sarah Anderson (12), Kelsey Ferrante (10), Kelsey Herbst (12) | McDowell High School | Erie | PA |
| 71 | 3:31.27 | Marissa Murphy (11), Taylor Folkertsma (10), Hannah Messer (11), Katie McCullough (12) | Forest Hills Central High School | Grand Rapids | MI |
| 72 | 3:31.29 | Katie Newcomer (11), Alex Brennan (11), Keely Simonson (9 ), Zoe Mattingly (9 ) | Zionsville Community High School | Zionsville | IN |
| 73 | 3:31.30 | Chloe Conjares (11), Danielle Romain (10), Bryeanne Ravettine (12), Lia Kunnapas (12) | Immaculate Heart Academy | Township of Washington | NJ |
| 74 | 3:31.43 | Meghan Cummings (9 ), Claire Ritzen (12), Katie Bootsma (10), Rachel Bootsma (9 ) | Eden Prairie High School | Eden Prairie | MN |
| 75 | 3:31.45 | Maya DiRado (11), Neda Kazemini (12), Karissa DeRousseau (10), Lauren Matevish (11) | Maria Carrillo High School | Santa Rosa | CA |
| 76 | 3:31.47 | Jaime Malandra (12), Emily Poland (10), Erica Reifinger (9 ), Kayla Grim (10) | Parkland High School | Allentown | PA |
| 77 | 3:31.56 | Heather O'Toole (12), Emily Kintz (12), Morgan Peterson (10), Haley Durmer (9 ) | St. Pius X Catholic High School | Atlanta | GA |
| 78 | 3:31.72 | Gretchen Stein (9 ), Deanna Sorenson (12), Elizabeth Mace (11), Olivia Harrell (11) | Glendale High School | Springfield | MO |
| 79 | 3:31.91 | Marissa Konicke (10), Shirley Wang (10), Kelsey Konicke (12), Deanna Marks (10) | Plano Senior High School | Plano | TX |
| 80 | 3:31.96 | Cassidy Lapp (10), Claire Singley (11), Taylor Cole (11), Chelsea Dunlap (11) | Southlake Carroll High School | Southlake | TX |
| 80 | 3:31.96 | Shaun Casey (10), Ginny Grams (10), Emily Grams (10), Kayla Derr (12) | Spruce Creek High School | Port Orange | FL |
| 82 | 3:32.04 | Ibby Simcox (9 ), Ashlan Higgins (12), Shelby Bolin (11), Eleanor Prewtit-Thomas (12) | Blue Springs High School | Blue Springs | MO |
| 83 | 3:32.05 | Sarah Koss (12), Kayla Effinger (9 ), Emily Moe (9 ), Kait Flederbach (11) | Westfield High School | Westfield | IN |
| 84 | 3:32.09 | Sommers Creed (10), Frederique Lefebvre (9 ), Rachael Dudley (9 ), Marlie Creed (12) | Mill Creek High School | Hoschton | GA |
| 85 | 3:32.11 | Hannah Stephenson (10), Erin Burke (12), Abby Vance (11), Allison Hendren (12) | Leesville Road High School | Raleigh | NC |
| 86 | 3:32.13 | Susan Bolt (12), Kate Dvornitsyn (11), Jillian Cordes (11), Kelly Hagerty (11) | Glenbrook South High School | Glenview | IL |
| 87 | 3:32.18 | Hannah Ferrin (12), Delaney Rolfe (12), Bolton Harris (12), Allison Arnold (11) | Highland Park High School | Dallas | TX |
| 88 | 3:32.26 | Amber McDermott (10), Courtney McDermott (11), Marina Pomar-Enders (12), Alana Pazevic (11) | H. M. Jackson High School | Mill Creek | WA |
| 89 | 3:32.42 | Stephanie Ovalle (11), Mariclaire Bresnahan (12), Morgan Bonk (12), Kelly Ryan (11) | Fenwick High School | Oak Park | IL |
| 90 | 3:32.45 | Sara Delay (11), Jaymee Krochka (11), Tittle Mia (11), Joann Liang (11) | Northgate High School | Walnut Creek | CA |
| 90 | 3:32.45 | Marissa Neel (9 ), Keri George (12), Taylor Nanfria (10), Allison Brown (9 ) | San Ramon Valley High School | Danville | CA |
| 92 | 3:32.56 | Sarah Denninghoff (11), Caitlin Wright (10), Courtney Dangremond (10), Leighanne Oliver (10) | Sabino High School | Tucson | AZ |
| 93 | 3:32.58 | Lauren Rigg (11), Emily Scraggs (11), Taylor Kitayama (11), Tasia DeMuth (12) | Fairview High School | Boulder | CO |
| 94 | 3:32.59 | Meg Anderson (12), Hillary Hahmann (11), Haley Hahmann (10), Rebecca Werdine (9 ) | H.B. Plant High School | Tampa | FL |
| 95 | 3:32.60 | Brittany Kent (12), Stephanie Long (12), Amy Katz (10), Lauren Massey (12) | North Central High School | Indianapolis | IN |
| 96 | 3:32.94 | Paige Sauder (12), Nicole Miller (10), Taylor Streid (12), Rebecca Stoughton (10) | Peoria Notre Dame High School | Peoria | IL |
| 97 | 3:32.95 | Carolyn Meier (9 ), Erin Regan (10), Kelly Ashmead (12), Megan Lafferty (12) | Upper Dublin High School | Ft. Washington | PA |
| 98 | 3:32.98 | Karie Eitel (12), Andrea Slouka (11), Shelly Patton (9 ), Julie Widmann (12) | Heritage High School | Littleton | CO |
| 99 | 3:33.01 | Skyler Doss (11), Paula Ferrara (9 ), Kathleen Gast (10), Samantha Moffitt (10) | Cedar Crest High School | Lebanon | PA |
| 100 | 3:33.03 | Andrea Baumgartner (11), Vicki Munson (11), Shelby Daeschner (12), Elena Carvell (12) | Waubonsie Valley High School | Aurora | IL |
| 101 | 3:33.08 | Olivia Lin (12), Rebecca Reed (12), Romy Landeck (9 ), Danielle Yoho (12) | Clements High School | Sugar Land | TX |
| 102 | 3:33.11 | Cori Gary (10), Colleen Konetzke (9 ), Devyn Phalen (12), Alexa Morris (10) | Ronald Reagan High School | San Antonio | TX |
| 103 | 3:33.12 | Molly Albrecht (12), Clark Heather (11), Chloe Portela (10), Morgan Flannigan (11) | Lawrence Free State High School | Lawrence | KS |
| 103 | 3:33.12 | Natalya Ares (9 ), Shannon Ridge (9 ), Maria Watkins (11), Yvonne Huo (10) | Winston Churchill High School | Potomac | MD |
| 105 | 3:33.13 | Emma Lawrenz (10), Katie Friendlander (10), Paige Bauernfeind (12), Molly Mason (11) | Minnetonka High School | Minnetonka | MN |
| 106 | 3:33.16 | Kayla Beckwith (12), Ashley Ryan (12), Katie Delaney (10), Grace Jarzemsky (11) | Middleton High School | Middleton | WI |
| 107 | 3:33.18 | Sarah Musial (12), Stephanie Graham (11), Corinne Talhouk (10), Kally Vanderbilt (9 ) | North Allegheny High School | Wexford | PA |
| 108 | 3:33.31 | Kinsey Kowalski (12), Cynthia Donovan (11), Lynn Brotherton (11), Breann McDowell (11) | Ursuline Academy | Cincinnati | OH |
| 109 | 3:33.33 | Courtney Funk (9 ), Olsen Serena (11), Maggi Beck (12), Dagny Knutson (11) | Minot High School | Minot | ND |
| 110 | 3:33.38 | Jillian Palmer (12), McKenzie Cassidy (11), Erin Black (12), Stephanie Peacock (11) | Bishop Verot Catholic High School | Ft. Myers | FL |
| 111 | 3:33.39 | Emma Szczupakiewicz (9 ), Jessica Wolf (9 ), Brittany Walsh (11), Sami Pochowski (9 ) | Muskego High School | Muskego | WI |
| 112 | 3:33.41 | Natalie Domeisen (10), Katie Vincett (12), Anne Marie Bilott (10), Margaux Georgiadis (11) | Shady Side Academy | Pittsburgh | PA |
| 113 | 3:33.42 | Jackie Mays (11), Ashley Fuchs (9 ), Kyrene Clarke (11), Jenna Gregoire (11) | Rampart High School | Colorado Springs | CO |
| 114 | 3:33.45 | Stephanie Kinsey (11), Kimberly Jerzerick (12), Caroline Finkbeiner (9 ), Adrienne Phillips (12) | Trinity High School | Camp Hill | PA |
| 115 | 3:33.47 | Elizabeth Murray (11), Christine La (11), Megan Nolet (11), Lauren Opatrny (11) | Saratoga High School | Saratoga | CA |
| 116 | 3:33.49 | Tia Totura (12), Jehan Atoe (12), Kirsten Milberg (12), Jessica Holley (10) | Rock Canyon High School | Highlands Ranch | CO |
| 117 | 3:33.50 | Laura Evans (12), Christine Barnum (12), Erin Calderoni (10), Jesse Evans (10) | Greenwich High School | Greenwich | CT |
| 118 | 3:33.53 | Elly Faulkner (10), Rebecca Sears (9 ), Marisa McGlynn (11), Miranda Mercke (9 ) | Sacred Heart Academy | Louisville | KY |
| 119 | 3:33.63 | Anji Shakya (11), Rachel Johnson (9 ), Ella Eser (11), Beverly Nguyen (9 ) | Davis Senior High School | Davis | CA |
| 120 | 3:33.64 | Riley Spitser (9 ), Kathryn Bowersox (9 ), Danielle Ortizharo (12), Katie Anderson (9 ) | Rancho Bernardo High School | San Diego | CA |
| 121 | 3:33.72 | Erika Ronning (11), Margaret Swanson (12), Megan Mc Nichols (12), Sarah Norris (12) | Lyons Township High School | La Grange | IL |
| 122 | 3:33.76 | Dani Barbiea (10), Devin Spencer (11), Jennifer Garzinski (10), Kellie Barbiea (10) | Springboro High School | Springboro | OH |
| 123 | 3:33.79 | Beth DeFauw (11), Rachel Roman (11), Molly Buynak (12), Katie Kelly (11) | Strongsville High School | Strongsville | OH |
| 124 | 3:33.86 | Kayla Noon (10), Erin Sosdian (11), Megan Reutzel (9 ), Elizabeth Nelson (12) | The Woodlands College Park High School | The Woodlands | TX |
| 125 | 3:33.87 | Jillian Homans (9 ), Elaine Pitsas (10), Brittany Gilbert (9 ), Stacey Wierman (11) | Munster High School | Munster | IN |
| 126 | 3:33.99 | Gabreille Mizerak (12), Audrey Kula (9 ), Carmen Young (10), Courtney Mizerak (9 ) | Osbourn Park High School | Manassas | VA |
| 127 | 3:34.15 | Kayley Dodson (11), Marilyn Carey (10), Darielle Watkins (10), Rachel Fattal (9 ) | Los Alamitos High School | Los Alamitos | CA |
| 127 | 3:34.15 | Alison Meng (10), Megan Cahill (11), Kelsey Kohlbeck (10), Abby Diehl (12) | Sauk Prairie High School | Prairie du Sac | WI |
| 129 | 3:34.16 | Katie Olsen (10), Lauren Torres (10), Taylor Newgaard (11), Tinsley Andrews (11) | Desert Vista High School | Phoenix | AZ |
| 129 | 3:34.16 | Paige Baisden (9 ), Codyanne Murtaugh (10), Kassandra McNichol (11), Lauren Baisden (12) | West Chester Henderson High School | West Chester | PA |