| Place | Time | Name (Yr) | School | City | State |
| 1 | :51.85 | Cindy Tran (12) | Edison High School | Huntington Beach | CA |
| 2 | :52.89 | Lily Moldenhauer (12) | Winston Churchill High School | San Antonio | TX |
| 3 | :53.04 | Missy Franklin (9 ) | Regis Jesuit High School | Aurora | CO |
| 4 | :53.33 | Deborah Roth (12) | Redwood High School | Corte Madera | CA |
| 5 | :53.53 | Courtney Bartholomew (10) | Holland High School | Holland | MI |
| 6 | :53.82 | Aubrey Peacock (12) | The Bolles School | Jacksonville | FL |
| 7 | :53.98 | Jillian Vitarius (10) | Kingwood High School | Kingwood | TX |
| 8 | :53.99 | Erin Reisinger (12) | Walton High School | Marietta | GA |
| 9 | :54.07 | Annemarie Thayer (11) | Ballard High School | Seattle | WA |
| 10 | :54.15 | Caitlyn Forman (10) | Notre Dame Academy | Park Hills | KY |
| 11 | :54.21 | Kate McNeilis (12) | Dublin Scioto High School | Dublin | OH |
| 12 | :54.29 | Krissie Brandenburg (12) | Beechwood High School | Fort Mitchell | KY |
| 13 | :54.40 | Sabrina Benson (11) | Broughton High School | Raleigh | NC |
| 14 | :54.47 | Tess Behrens (12) | Maple Grove Senior High School | Maple Grove | MN |
| 14 | :54.47 | Madison White (9 ) | Carondelet High School | Concord | CA |
| 16 | :54.48 | Mary Hanson (12) | Immanuel Christian School | Ridgecrest | CA |
| 17 | :54.60 | Lacey Locke (9 ) | Carmel High School | Carmel | IN |
| 18 | :54.65 | Arden Pitman (11) | The Baylor School | Chattanooga | TN |
| 19 | :54.73 | Madison Bridges (10) | Albuquerque High School | Albuquerque | NM |
| 20 | :54.81 | Hailey Campbell (12) | Lakeville South High School | Lakeville | MN |
| 21 | :54.82 | Samantha Taylor (12) | Capistrano Valley High School | Mission Viejo | CA |
| 22 | :54.89 | Wylie Escobedo (12) | Austin High School | Austin | TX |
| 22 | :54.89 | Kendyl Stewart (10) | La Costa Canyon High School | Carlsbad | CA |
| 24 | :54.94 | Alana Pazevic (12) | Henry M. Jackson High School | Mill Creek | WA |
| 25 | :55.05 | Linnea Mack (9 ) | Pioneer High School | San Jose | CA |
| 26 | :55.15 | Christina Leander (9 ) | West Chester East High School | West Chester | PA |
| 27 | :55.22 | Marissa Neel (10) | San Ramon Valley High School | Danville | CA |
| 27 | :55.22 | Karen Turner (10) | Lakeridge High School | Lake Oswego | OR |
| 29 | :55.42 | Kelly Ryan (12) | Fenwick High School | Oak Park | IL |
| 30 | :55.44 | Lauren Solernou (12) | Sacred Heart High School | Waterbury | CT |
| 31 | :55.51 | Yurie Nakano (11) | Hershey High School | Hershey | PA |
| 32 | :55.55 | Riley Flanagan (12) | Buchholz High School | Gainesville | FL |
| 33 | :55.57 | Kaitlyn Jones (9 ) | The Tatnall School | Wilmington | DE |
| 33 | :55.57 | Olivia Smoliga (9 ) | Glenbrook South High School | Glenview | IL |
| 35 | :55.58 | Nicole Stafford (9 ) | Westminster Schools | Atlanta | GA |
| 36 | :55.62 | Melanie McClure (12) | Mansfield High School | Mansfield | TX |
| 37 | :55.63 | Taylor Sneed (11) | Germantown Academy | Fort Washington | PA |
| 38 | :55.64 | Kim Pavlin (12) | Winston Churchill High School | San Antonio | TX |
| 39 | :55.69 | Emily Bos (12) | Holland High School | Holland | MI |
| 40 | :55.72 | Haley Preston (10) | Westlake High School | Westlake Village | CA |
| 41 | :55.74 | Cara Chuang (12) | Poolesville High School | Poolesville | MD |
| 42 | :55.77 | Melanie Busch (11) | William Tennent High School | Warminster | PA |
| 42 | :55.77 | Shirley Wang (11) | Plano Senior High School | Plano | TX |
| 44 | :55.80 | Rachel Moore (10) | Andover High School | Andover | MA |
| 45 | :55.81 | Lauren Piper (12) | Northeast High School | St. Petersburg | FL |
| 46 | :55.86 | Brittany Fozkos (12) | Chesterton High School | Chesterton | IN |
| 47 | :55.87 | Kaila N. Lee (12) | Willamette High School | Eugene | OR |
| 48 | :55.96 | Dagny Knutson (12) | Minot High School | Minot | ND |
| 49 | :55.99 | Ashlee Linn (10) | Riverview High School | Sarasota | FL |
| 50 | :56.00 | Rachel Stearns (12) | Hopkins High School | Hopkins | MN |
| 51 | :56.01 | Brittany Gilbert (10) | Munster High School | Munster | IN |
| 52 | :56.07 | Andrea VanderMey (9 ) | Novi High School | Novi | MI |
| 53 | :56.09 | Kathryn Eckhart (10) | Cypress Creek High School | Houston | TX |
| 54 | :56.10 | Lauren Opatrny (12) | Saratoga High School | Saratoga | CA |
| 55 | :56.11 | Megan McCarroll (11) | Jesuit High School | Portland | OR |
| 56 | :56.13 | Chelsea Bailey (11) | Kentlake Senior High School | Kent | WA |
| 57 | :56.16 | Anna Gibas (12) | Greater Latrobe High School | Latrobe | PA |
| 57 | :56.16 | Samantha Schurig (9 ) | Washburn Rural High School | Topeka | KS |
| 59 | :56.23 | Jane Munro (12) | Evanston Township High School | Evanston | IL |
| 60 | :56.25 | Elizabeth Beisel (12) | North Kingstown High School | North Kingstown | RI |
| 60 | :56.25 | Lauren Damian (11) | Monte Vista High School | Danville | CA |
| 62 | :56.26 | Alexis Bullard (10) | Carmel High School | Carmel | IN |
| 63 | :56.27 | Paige Impink (12) | Wilson High School | West Lawn | PA |
| 64 | :56.33 | Olivia Scott (12) | Rosary High School | Aurora | IL |
| 65 | :56.34 | Mallory Miles (12) | Southridge High School | Huntingburg | IN |
| 66 | :56.35 | Shelly Patton (10) | Heritage High School | Littleton | CO |
| 67 | :56.39 | Amy Katz (11) | North Central High School Indianapolis | Indianapolis | IN |
| 68 | :56.42 | Sydney Johansen (11) | St. Francis High School | Sacramento | CA |
| 69 | :56.45 | Ali Polhill (12) | Brewster High School | Brewster | NY |
| 70 | :56.46 | Kristyn Fulcher (12) | Upper Arlington High School | Upper Arlington | OH |
| 71 | :56.48 | Bonnie Brandon (10) | Cherry Creek High School | Greenwood Village | CO |
| 72 | :56.50 | Berklie Oscarson (12) | San Marino High School | San Marino | CA |
| 73 | :56.51 | Maggie Rosenbaum (12) | Monument Mountain Regional High School | Great Barrington | MA |
| 73 | :56.51 | Brooke Waugaman (11) | Hempfield Area High School | Greensburg | PA |
| 75 | :56.55 | Kelly Naze (10) | Cherry Creek High School | Greenwood Village | CO |
| 76 | :56.58 | Andrea Marko (12) | Greensburg Central Catholic High School | Greensburg | PA |
| 77 | :56.60 | Talie Anchustegui (10) | Xavier College Preparatory | Phoenix | AZ |
| 78 | :56.63 | Katie Duggan (11) | Wyomissing Area High School | Wyomissing | PA |
| 78 | :56.63 | Eva Greene (11) | McLean High School | McLean | VA |
| 78 | :56.63 | Stephanie Schlobohm (10) | Aliso Niguel High School | Aliso Viejo | CA |
| 78 | :56.63 | Danielle Yoon (9 ) | Memorial High School | Houston | TX |
| 82 | :56.65 | Marissa Cominelli (9 ) | Hawken School | Gates Mills | OH |
| 83 | :56.66 | Stephanie Ah-Quah (12) | South Pasadena High School | South Pasadena | CA |
| 83 | :56.66 | Kelsey Ferrante (11) | McDowell High School | Erie | PA |
| 85 | :56.67 | Kayla Grim (11) | Parkland High School | Allentown | PA |
| 85 | :56.67 | Mackenzie Powers (12) | Rosary High School | Aurora | IL |
| 87 | :56.68 | Larah Beaver (10) | Yorktown High School | Yorktown | IN |
| 87 | :56.68 | Katie Bootsma (11) | Eden Prairie High School | Eden Prairie | MN |
| 87 | :56.68 | Samantha Wicks (10) | East High School | Anchorage | AK |
| 90 | :56.69 | Erin Gaeckle (11) | Charlotte Catholic High School | Charlotte | NC |
| 91 | :56.70 | Kyra Jones (12) | Seven Lakes High School | Katy | TX |
| 92 | :56.71 | Anji Shakya (12) | Davis Senior High School | Davis | CA |
| 93 | :56.73 | Kati Kyle (12) | Joe E. Newsome High School | Lithia | FL |
| 94 | :56.74 | Gigi Groya (11) | Lyons Township High School | Lagrange | IL |
| 95 | :56.75 | Sarah Denninghoff (12) | Sabino High School | Tucson | AZ |
| 96 | :56.76 | Maddy Schaefer (11) | Saint Francis High School | Mountain View | CA |
| 97 | :56.78 | Madeleine Klichowski (11) | Loyola Academy | Wilmette | IL |
| 97 | :56.78 | Kasey Morris (12) | Oscar Smith High School | Chesapeake | VA |
| 99 | :56.80 | Sophie Weber (10) | Granite Bay High School | Granite Bay | CA |
| 100 | :56.85 | Nina Damjanovich (12) | Munster High School | Munster | IN |
| 101 | :56.87 | Jesse Bergman (12) | Homestead High School | Cupertino | CA |
| 101 | :56.87 | Ashley Brewer (12) | Chaparral High School | Scottsdale | AZ |
| 101 | :56.87 | Kelsey Williams (11) | Danville Area High School | Danville | PA |
| 104 | :56.88 | Rachel Kim (11) | Todd Beamer High School | Federal Way | WA |
| 105 | :56.90 | Rebecca Baxley (9 ) | Analy High School | Sebastopol | CA |
| 105 | :56.90 | Morgan Sprosty (12) | Northwood High School | Irvine | CA |
| 107 | :56.94 | Sidney Cooke (9 ) | Monte Vista High School | Danville | CA |
| 108 | :56.97 | Lauren Rosenberg (12) | Olentangy Liberty High School | Powell | OH |
| 109 | :57.00 | Anna DeMonte (10) | Ann Arbor Huron High School | Ann Arbor | MI |
| 110 | :57.02 | Alexandra Lundgren (11) | The Bolles School | Jacksonville | FL |
| 111 | :57.04 | Chandler Soapes (9 ) | Episcopal High School | Jacksonville | FL |
| 111 | :57.04 | Arlyn Upshaw (10) | Piedmont High School | Piedmont | CA |
| 113 | :57.05 | Lauren Comer (10) | Upper Arlington High School | Upper Arlington | OH |
| 114 | :57.06 | Christina Bechtel (11) | Franklin High School | Franklin | TN |
| 115 | :57.07 | Maria Bargardi (11) | Mercy High School | Farmington Hills | MI |
| 115 | :57.07 | Libby Hunsche (12) | Turpin High School | Cincinnati | OH |
| 117 | :57.08 | Nikki Cannon (11) | Hudson's Bay High School | Vancouver | WA |
| 117 | :57.08 | Nina Hayes (9 ) | Granada High School | Livermore | CA |
| 119 | :57.09 | Lauren Harrington (11) | St. Mary's Episcopal School | Memphis | TN |
| 120 | :57.10 | Stephanie Nguyen (12) | Lynbrook High School | San Jose | CA |
| 121 | :57.11 | Suzanne Bessire (11) | Winter Park High School | Winter Park | FL |
| 121 | :57.11 | Kaitlin Mills (12) | Our Lady of Good Counsel | Olney | MD |
| 123 | :57.13 | Taylor Derevyanik (10) | Southlake Carroll High School | Southlake | TX |
| 123 | :57.13 | Kearsten Livingstone (12) | Canyon High School | Canyon Country | CA |
| 125 | :57.18 | Lauren Davis (11) | Westwood High School | Austin | TX |
| 125 | :57.18 | Molly Smyth (11) | Cromwell High School | Cromwell | CT |
| 127 | :57.19 | Alison Meng (11) | Sauk Prairie High School | Prairie du Sac | WI |
| 128 | :57.20 | Allie Sullins (10) | Magnolia West High School | Magnolia | TX |
| 128 | :57.20 | Kristen Vredeveld (9 ) | The Baylor School | Chattanooga | TN |
| 130 | :57.21 | Yana Garvey (12) | Mountain Vista High School | Highlands Ranch | CO |
| 130 | :57.21 | Alena Kluge (12) | Emmaus High School | Emmaus | PA |
| 132 | :57.24 | Marisa McGlynn (12) | Sacred Heart Academy | Louisville | KY |
| 133 | :57.25 | Jenna Bickel (10) | Guilderland High School | Guilderland Center | NY |
| 133 | :57.25 | Aubrey Hertzler (12) | Hamilton Southeastern High School | Fishers | IN |
| 135 | :57.26 | Kelsi Worrell (10) | Rancocas Valley Regional High School | Mt. Holly | NJ |
| 136 | :57.27 | Margaret Cooke (9 ) | The Woodlands High School | The Woodlands | TX |
| 136 | :57.27 | Chloe Portela (11) | Lawrence Free State High School | Lawrence | KS |
| 138 | :57.29 | Heather Coutts (11) | Bishop Kenny High School | Jacksonville | FL |
| 138 | :57.29 | Claudia Doyle (11) | Thomas Worthington High School | Worthington | OH |
| 138 | :57.29 | Kylie Garcia (12) | John A. Rowland High School | Rowland Heights | CA |