| Place | Time | Name (Yr) | School | City | State |
| 1 | :48.15 | Dagny Knutson (12) | Minot High School | Minot | ND |
| 2 | :48.28 | Missy Franklin (9 ) | Regis Jesuit High School | Aurora | CO |
| 3 | :48.45 | Margo Geer (12) | Fairbanks High School | Milford Center | OH |
| 4 | :48.61 | Maddy Schaefer (11) | Saint Francis High School | Mountain View | CA |
| 5 | :49.70 | Samantha Tucker (12) | Westlake High School | Austin | TX |
| 6 | :49.82 | Maya DiRado (12) | Maria Carrillo High School | Santa Rosa | CA |
| 7 | :49.93 | Kathryn Murphy (12) | Marin Catholic High School | Kentfield | CA |
| 8 | :49.94 | Aja Van Hout (11) | Madison East High School | Madison | WI |
| 9 | :49.96 | Emily Bos (12) | Holland High School | Holland | MI |
| 10 | :50.06 | Gracie Finnegan (12) | Bishop Watterson High School | Columbus | OH |
| 11 | :50.08 | Kristen Vredeveld (9 ) | The Baylor School | Chattanooga | TN |
| 12 | :50.17 | Maddie Locus (11) | Dulles High School | Sugar Land | TX |
| 13 | :50.20 | Kait Flederbach (12) | Westfield High School | Westfield | IN |
| 13 | :50.20 | Jasmine Tosky (10) | Palo Alto High School | Palo Alto | CA |
| 15 | :50.23 | Kate McNeilis (12) | Dublin Scioto High School | Dublin | OH |
| 16 | :50.26 | Hannah Bowen (11) | Stillwater Area High School | Stillwater | MN |
| 16 | :50.26 | Lauren Harrington (11) | St. Mary's Episcopal School | Memphis | TN |
| 18 | :50.27 | Deborah Roth (12) | Redwood High School | Corte Madera | CA |
| 19 | :50.29 | Danielle Siverling (11) | Downingtown East High School | Exton | PA |
| 20 | :50.30 | Elizabeth Beisel (12) | North Kingstown High School | North Kingstown | RI |
| 21 | :50.35 | Kaitlin Frehling (12) | Seminole High School | Seminole | FL |
| 22 | :50.44 | Kaylin Bing (11) | Roseburg High School | Roseburg | OR |
| 23 | :50.47 | Haley Krakoski (12) | Xavier College Preparatory | Phoenix | AZ |
| 24 | :50.48 | Sara Delay (12) | Northgate High School | Walnut Creek | CA |
| 25 | :50.53 | Kelsey Leneave (11) | Conroe High School | Conroe | TX |
| 26 | :50.54 | Lindsay Gendron (12) | Normal Community High School | Normal | IL |
| 27 | :50.56 | Kelli Benjamin (11) | Gilbert High School | Gilbert | AZ |
| 28 | :50.59 | Krissie Brandenburg (12) | Beechwood High School | Fort Mitchell | KY |
| 29 | :50.61 | Casey Clark (11) | Klein High School | Klein | TX |
| 30 | :50.63 | Dani Barbiea (11) | Springboro High School | Springboro | OH |
| 30 | :50.63 | Carly Mercer (12) | Hamilton Southeastern High School | Fishers | IN |
| 32 | :50.65 | Mary Moser (12) | Wyomissing Area High School | Wyomissing | PA |
| 33 | :50.67 | Jordan Mattern (11) | Cherry Creek High School | Greenwood Village | CO |
| 34 | :50.72 | Molly Osterhage (12) | Centerville High School | Centerville | OH |
| 35 | :50.74 | Shelby Fortin (12) | Weston High School | Weston | CT |
| 36 | :50.82 | Da Yae Choi (10) | Woodbridge High | Irvine | CA |
| 36 | :50.82 | Jackie Powell (12) | Madison Memorial High School | Madison | WI |
| 38 | :50.85 | Megan Fonteno (11) | The Bolles School | Jacksonville | FL |
| 38 | :50.85 | Faith Martin (11) | St. Paul Catholic High School | Bristol | CT |
| 40 | :50.87 | Andrea Grant (11) | Walnut High School | Walnut | CA |
| 40 | :50.87 | Courtney Wilde (11) | Laguna Hills High School | Laguna Hills | CA |
| 42 | :50.89 | Madison White (9 ) | Carondelet High School | Concord | CA |
| 43 | :50.90 | Amelia Schachter (10) | Arroyo Grande High School | Arroyo Grande | CA |
| 44 | :50.91 | Annie Nunes (9 ) | Charter School of Wilmington | Wilmington | DE |
| 45 | :50.92 | Wylie Escobedo (12) | Austin High School | Austin | TX |
| 45 | :50.92 | Danielle Jansen (12) | Mason High School | Mason | OH |
| 47 | :50.98 | Hailey Campbell (12) | Lakeville South High School | Lakeville | MN |
| 48 | :50.99 | Emily Lloyd (12) | Severna Park High School | Severna Park | MD |
| 49 | :51.01 | Melissa Bates (12) | Mater Dei High School | Santa Ana | CA |
| 49 | :51.01 | Jenna Gregoire (12) | Rampart High School | Colorado Springs | CO |
| 49 | :51.01 | Melanie Klaren (11) | Aliso Niguel High School | Aliso Viejo | CA |
| 52 | :51.03 | Samantha Zuch (12) | Napoleon High School | Napoleon | OH |
| 53 | :51.04 | Catherine Breed (11) | Amador Valley High School | Pleasanton | CA |
| 54 | :51.05 | Sara Li (12) | Longmeadow High School | Longmeadow | MA |
| 55 | :51.07 | Caitlyn Karr (11) | Wilson High School | West Lawn | PA |
| 56 | :51.09 | Caroline Piehl (11) | Smoky Hill High School | Aurora | CO |
| 57 | :51.11 | Megan Lydzinski (10) | Montgomery High School | Skillman | NJ |
| 58 | :51.13 | Bonnie Brandon (10) | Cherry Creek High School | Greenwood Village | CO |
| 58 | :51.13 | Caroline Thomas (11) | Dupont Manual High School | Louisville | KY |
| 60 | :51.15 | Kristen Wilson (12) | Paul Laurence Dunbar High School | Lexington | KY |
| 61 | :51.16 | Rachel Moore (10) | Andover High School | Andover | MA |
| 62 | :51.19 | Mikelle Masciantonio (12) | Emmaus High School | Emmaus | PA |
| 62 | :51.19 | Alexa Morris (11) | Ronald Reagan High School | San Antonio | TX |
| 64 | :51.20 | Caroline Kenney (12) | North Gwinnett High School | Suwanee | GA |
| 65 | :51.21 | Michelle Gean (12) | Kingwood High School | Kingwood | TX |
| 65 | :51.21 | Patricia Lesson (12) | Liberty High School | Bethlehem | PA |
| 67 | :51.22 | Chelsea Fong (12) | Monta Vista High School | Cupertino | CA |
| 67 | :51.22 | Zoe Mattingly (10) | Zionsville Community High School | Zionsville | IN |
| 69 | :51.23 | Julia Ama (10) | Gunn High School | Palo Alto | CA |
| 69 | :51.23 | Olivia Kabacinski (10) | Chesterton High School | Chesterton | IN |
| 69 | :51.23 | Julianne Phillips (11) | Western Branch High School | Chesapeake | VA |
| 69 | :51.23 | Emily Ryczek (11) | Sherwood High School | Sandy Spring | MD |
| 73 | :51.24 | Suzanne Bessire (11) | Winter Park High School | Winter Park | FL |
| 74 | :51.25 | Kristen Clarno (10) | Homestead High School | Cupertino | CA |
| 75 | :51.27 | Emily Cameron (9 ) | Warwick High School | Lititz | PA |
| 76 | :51.29 | Carolyn Fittin (11) | The Peddie School | Hightstown | NJ |
| 76 | :51.29 | Kim Pavlin (12) | Winston Churchill High School | San Antonio | TX |
| 78 | :51.34 | Maddy Dalcamo (12) | Olathe Northwest High School | Olathe | KS |
| 78 | :51.34 | Linnea Mack (9 ) | Pioneer High School | San Jose | CA |
| 80 | :51.35 | Kelsey R Buckley (10) | Tarpon Springs High School | Tarpon Springs | FL |
| 81 | :51.36 | Rachel Dixon (12) | Las Cruces High School | Las Cruces | NM |
| 81 | :51.36 | Ginny Grams (11) | Spruce Creek High School | Port Orange | FL |
| 81 | :51.36 | Kat Simonovic (9 ) | Mountain View High School | Mesa | AZ |
| 84 | :51.38 | Samantha James (10) | New Trier High School | Winnetka | IL |
| 85 | :51.39 | Alli Gillespie (11) | Edison High School | Huntington Beach | CA |
| 86 | :51.41 | Hanna Cowley (11) | Ann Arbor Pioneer High School | Ann Arbor | MI |
| 86 | :51.41 | Cristina Gonzalez (12) | Portage High School | Portage | IN |
| 88 | :51.42 | Faith Johnson (10) | High Point Christian Academy | High Point | NC |
| 89 | :51.43 | Sarah Aemisegger (11) | Germantown Academy | Fort Washington | PA |
| 90 | :51.45 | Yurie Nakano (11) | Hershey High School | Hershey | PA |
| 91 | :51.47 | Courtney Pope (12) | Lockport Township High School | Lockport | IL |
| 92 | :51.48 | Katie Joseph (11) | McDonald High school | McDonald | OH |
| 92 | :51.48 | Mikkel-Ane Stipe (12) | La Costa Canyon High School | Carlsbad | CA |
| 94 | :51.50 | Mariah Tharp (12) | Clovis West High School | Fresno | CA |
| 94 | :51.50 | Maddy Urban (12) | New Trier High School | Winnetka | IL |
| 96 | :51.51 | Tristin Baxter (12) | Clovis West High School | Fresno | CA |
| 96 | :51.51 | Haley Pietila (10) | Arrowhead High School | Hartland | WI |
| 98 | :51.55 | Missy Doll (12) | Souderton Area High School | Souderton | PA |
| 99 | :51.56 | Claire Brandt (10) | Greenhill School | Addison | TX |
| 99 | :51.56 | M.E. Randall (12) | College Park High School | Pleasant Hill | CA |
| 101 | :51.57 | Amber Cratsenberg (10) | Thomas Jefferson High School | Auburn | WA |
| 102 | :51.58 | Julia Anderson (10) | R. L. Paschal High School | Fort Worth | TX |
| 103 | :51.59 | Hillary Thomas (11) | Regis Jesuit High School | Aurora | CO |
| 104 | :51.61 | Eva Greene (11) | McLean High School | McLean | VA |
| 104 | :51.61 | Victoria Palombit (11) | Coronado High School | Lubbock | TX |
| 106 | :51.62 | Kelly Hagerty (12) | Glenbrook South High School | Glenview | IL |
| 106 | :51.62 | Jessica Marsh (11) | Whittier High School | Whittier | CA |
| 108 | :51.67 | Cassie Niles (11) | Sandra Day O'Connor High School | Helotes | TX |
| 109 | :51.69 | Lindsey LaPorte (12) | Pinnacle High School | Phoenix | AZ |
| 110 | :51.71 | Samantha Taylor (12) | Capistrano Valley High School | Mission Viejo | CA |
| 111 | :51.72 | Alexis Bullard (10) | Carmel High School | Carmel | IN |
| 111 | :51.72 | Anji Shakya (12) | Davis Senior High School | Davis | CA |
| 113 | :51.74 | Michaela Kearney (11) | Mercy High School | Middletown | CT |
| 114 | :51.76 | Kelly Naze (10) | Cherry Creek High School | Greenwood Village | CO |
| 114 | :51.76 | Camey Rabold (10) | Bishop Watterson High School | Columbus | OH |
| 114 | :51.76 | Stacey Stanfield (10) | Creekside High School | St. Johns | FL |
| 117 | :51.78 | Ellie Morrissey (10) | Edison High School | Huntington Beach | CA |
| 118 | :51.79 | Samantha Bosma (11) | Lassiter High School | Marietta | GA |
| 118 | :51.79 | Sarah Denninghoff (12) | Sabino High School | Tucson | AZ |
| 120 | :51.80 | Harper Bruens (10) | Boca Raton High School | Boca Raton | FL |
| 121 | :51.83 | Brittany Friese (9 ) | Newburgh Free Academy | Newburgh | NY |
| 122 | :51.85 | Kelsey Engel (12) | El Toro High School | Lake Forest | CA |
| 122 | :51.85 | Katelyne Herrington (12) | Tokay High School | Lodi | CA |
| 122 | :51.85 | Kelsey McMurtry (12) | Minnetonka High School | Minnetonka | MN |
| 125 | :51.86 | Kiera Janzen (11) | Cretin-Derham Hall High School | St. Paul | MN |
| 125 | :51.86 | Shaya Schaedler (10) | Campbell County High School | Gillette | WY |
| 125 | :51.86 | Summer Strickler (12) | H. H. Dow High School | Midland | MI |
| 125 | :51.86 | Briana Wilford (12) | Catalina Foothills High School | Tucson | AZ |
| 129 | :51.87 | Rachael Acker (10) | Gunn High School | Palo Alto | CA |
| 129 | :51.87 | Tess Behrens (12) | Maple Grove Senior High School | Maple Grove | MN |
| 129 | :51.87 | Kelsey Gouge (10) | Brookwood High School | Snellville | GA |