| Place | Time | Names (Yrs) | School | City | State |
| 1 | 3:23.86 | Alli Gilespie (11), Ellie Morrissey (10), Nicole Boyce (11), Cindy Tran (12) | Edison High School | Huntington Beach | CA |
| 2 | 3:24.39 | Bonnie Brandon (10), Jordan Mattern (11), Katie Naze (12), Kelly Naze (10) | Cherry Creek High School | Greenwood Village | CO |
| 3 | 3:24.43 | Missy Franklin (9 ), Alex Todd (11), Megan Grier (9 ), Hillary Thomas (11) | Regis Jesuit High School | Aurora | CO |
| 4 | 3:25.14 | Alexis Bullard (10), Rhiannon Sheets (11), Margaret Ramsey (10), Trish Regan (12) | Carmel High School | Carmel | IN |
| 5 | 3:25.37 | Megan Fonteno (11), Dana Nessler (12), Alexandra Lundgren (11), Aubrey Peacock (12) | The Bolles School | Jacksonville | FL |
| 6 | 3:25.97 | Maddy Schaefer (11), Paige Gilley (12), Jessie Ho (9 ), Jacqueline Levere (11) | Saint Francis High School | Mountain View | CA |
| 7 | 3:26.56 | Aviry Reich (12), Paige Copeland (12), Aubrey Hertzler (12), Carly Mercer (12) | Hamilton Southeastern High School | Fishers | IN |
| 8 | 3:26.65 | Deirdre Clute (12), Christine Tormey (12), Madison White (9 ), Chelsea Chenault (9 ) | Carondelet High School | Concord | CA |
| 9 | 3:26.96 | Mackenzie Franklin (9 ), Ruth Norval (12), Maddie Edwards (9 ), Samantha Tucker (12) | Westlake High School | Austin | TX |
| 10 | 3:27.07 | Kacie Ciampoli (11), Angie Sawick (11), Bridget Casey (11), Sarah Aemisegger (11) | Germantown Academy | Fort Washington | PA |
| 11 | 3:27.09 | Camey Rabold (10), Michele Rielly (10), Samantha Schuttinger (9 ), Gracie Finnegan (12) | Bishop Watterson High School | Columbus | OH |
| 12 | 3:27.14 | Camilla Czulada (10), Paige Impink (12), Taylor Brew (10), Caitlyn Karr (11) | Wilson High School | West Lawn | PA |
| 13 | 3:27.39 | Nicole Cordero (10), Haley Thompson (10), Courtney Bartholomew (10), Emily Bos (12) | Holland High School | Holland | MI |
| 14 | 3:27.63 | Maddy Urban (12), Megan Marchuk (11), Morgan Scott (10), Samantha James (10) | New Trier High School | Winnetka | IL |
| 15 | 3:27.64 | Kaela Anderson (12), Monica Radecke (12), Claire Rutscher (9 ), Hannah Bowen (11) | Stillwater Area High School | Stillwater | MN |
| 16 | 3:28.20 | Sarah Liang (11), Margaret Wenzlau (10), Sabrina Lee (11), Jasmine Tosky (10) | Palo Alto High School | Palo Alto | CA |
| 17 | 3:28.57 | Sarah Barker (10), Clark Weber (12), Sarah Love (9 ), Caroline Thomas (11) | Dupont Manual High School | Louisville | KY |
| 18 | 3:28.65 | Aja Van Hout (11), Ivy Martin (11), Chelsea Soderholm (10), Becca Soderholm (12) | Madison East High School | Madison | WI |
| 19 | 3:28.82 | Talie Anchustegui (10), Alisha Bender (10), Keenan Elizabeth (12), Haley Krakoski (12) | Xavier College Preparatory | Phoenix | AZ |
| 20 | 3:28.87 | Aubree Allen (10), Breeja Larson (12), Kat Simonovic (9 ), Emily Christainsen (12) | Mountain View High School | Mesa | AZ |
| 21 | 3:28.88 | Rachael Acker (10), Teva Levens (12), Alex Lincoln (12), Julia Ama (10) | Gunn High School | Palo Alto | CA |
| 22 | 3:28.95 | Sarah Sykstus (10), Rachel Burke (10), Emily Launer (10), Diana Norkus (12) | Rosary High School | Aurora | IL |
| 23 | 3:29.05 | Kate Jones (10), Emma Goral (11), Haley Pietila (10), Julia Mikota (9 ) | Arrowhead High School | Hartland | WI |
| 24 | 3:29.24 | Jillian Vitarius (10), Katheryn Nolen (9 ), Madeline Hazle (9 ), Michelle Gean (12) | Kingwood High School | Kingwood | TX |
| 25 | 3:29.25 | Megan Cox (10), Ashley Brewer (12), Malia Johnson (12), Leticia Lelli (10) | Chaparral High School | Scottsdale | AZ |
| 26 | 3:29.28 | Lauren Comer (10), Kayla Hammerberg (11), Kristyn Fulcher (12), Abigail Chin (11) | Upper Arlington High School | Upper Arlington | OH |
| 27 | 3:29.29 | Yurie Nakano (11), Katelyn Miller (11), Alexis Grier (10), Samantha Surfus (9 ) | Hershey High School | Hershey | PA |
| 28 | 3:29.34 | Kim Pavlin (12), Rebekah Pauly (10), Davina Jaynes (9 ), Lily Moldenhauer (12) | Winston Churchill High School | San Antonio | TX |
| 29 | 3:29.42 | Brittany Gilbert (10), Elaine Pitsas (11), Jillian Homans (10), Stacey Wierman (12) | Munster High School | Munster | IN |
| 30 | 3:29.64 | Emma Lawrenz (11), Katie Friedlander (11), Kelsey McMurtry (12), Molly Mason (12) | Minnetonka High School | Minnetonka | MN |
| 31 | 3:29.70 | Allison Brown (10), Jennifer Barcellona (9 ), Taylor Nanfria (11), Marissa Neel (10) | San Ramon Valley High School | Danville | CA |
| 32 | 3:29.77 | Lauren McCandless (12), Alena Kluge (12), Catherine Carr (12), Mikelle Masciantonio (12) | Emmaus High School | Emmaus | PA |
| 33 | 3:29.86 | Katie Newcomer (12), Alex Brennan1 (12), Lizzie Lyon (10), Zoe Mattingly (10) | Zionsville Community High School | Zionsville | IN |
| 34 | 3:29.93 | Rachel Cleak (12), Andrea Ward (12), Meaghan Bicomong (9 ), Courtney Whyte (11) | Campolindo High School | Moraga | CA |
| 35 | 3:29.95 | Reia Tong (10), Lauren Poore (12), Charlotte Meyer (9 ), Danielle Schulkin (12) | Walt Whitman High School | Bethesda | MD |
| 36 | 3:29.97 | Summer Stephens (11), Marisa McGlynn (12), Elly Faulkner (11), Rebecca Sears (10) | Sacred Heart Academy | Louisville | KY |
| 37 | 3:30.00 | Stephanie Ovalle (12), Casey Dollard (12), Maureen Barron (10), Kelly Ryan (12) | Fenwick High School | Oak Park | IL |
| 38 | 3:30.07 | Maya DiRado (12), Lauren Matevish (12), Karissa DeRousseau (11), Alexandria Holland (12) | Maria Carrillo High School | Santa Rosa | CA |
| 39 | 3:30.08 | Fallon Fitzpatrick (12), Colleen Williams (10), Danielle Smith (12), Harper Bruens (10) | Boca Raton High School | Boca Raton | FL |
| 40 | 3:30.16 | Antoinette Ranit (11), Carissa Metcalf (11), Sydney Johansen (11), Cora Stebbins (10) | St. Francis High School | Sacramento | CA |
| 41 | 3:30.22 | Cheyenne Low (9 ), Molly Jubas (11), Lauren Parrett (12), Megan Rankin (10) | University High School | Irvine | CA |
| 42 | 3:30.24 | Carolyn Fittin (11), Molly Mucciarone (10), Rachel Flinn (12), Laura Loughran (12) | The Peddie School | Hightstown | NJ |
| 43 | 3:30.36 | Heather Arseth (11), Emma Paulson (9 ), Jacqueline Stenroos (12), Sarah Deis (11) | Wayzata High School | Plymouth | MN |
| 44 | 3:30.45 | Casey Clark (11), Erica Dittmer (12), Jordan Smith (10), Jordan Domeier (9 ) | Klein High School | Klein | TX |
| 45 | 3:30.53 | Deanna Marks (11), Shirley Wang (11), Shannon Rogers (10), Marissa Konicke (11) | Plano Senior High School | Plano | TX |
| 46 | 3:30.54 | Alexis Walley (11), Erica Reifinger (10), Kayla Grim (11), Emily Stang (10) | Parkland High School | Allentown | PA |
| 47 | 3:30.60 | Ashley Evans (12), Kacey Milligan (11), Lindsey LaPorte (12), Jennifer LaPorte (10) | Pinnacle High School | Phoenix | AZ |
| 48 | 3:30.62 | Kaitlyn Van Peursem (9 ), Genevieve Bradford (10), Aya Fujimura (12), Dayae Choi (10) | Woodbridge High | Irvine | CA |
| 49 | 3:30.69 | Milana Socha (12), Julia Campbell (9 ), Maddie Miller (10), Olivia Kabacinski (10) | Chesterton High School | Chesterton | IN |
| 50 | 3:30.71 | Courtney Ciesielski (12), Ansley Crenshaw (12), Sarah Williford (10), Samantha Bosma (11) | Lassiter High School | Marietta | GA |
| 51 | 3:30.73 | Kyra Jones (12), Charlotte Lechner (10), Anne Steele (11), Bri Dillon (11) | Seven Lakes High School | Katy | TX |
| 52 | 3:30.82 | Sarah Denninghoff (12), Caitlin Wright (11), Courtney Dangremond (11), LeighAnne Oliver (11) | Sabino High School | Tucson | AZ |
| 53 | 3:30.84 | Mariah Tharp (12), Mackenzie Schuler (11), Lauren Martin (11), Tristin Baxter (12) | Clovis West High School | Fresno | CA |
| 54 | 3:31.04 | Sarah Koucheki (9 ), Carrie Bencic (9 ), Jordan Bitterman (10), Morgan Cohara (10) | Hawken School | Gates Mills | OH |
| 55 | 3:31.13 | Anna Rae Gwarjanski (12), Savannah Napier (11), Bria Deveaux (10), Emma Michaels (11) | The Baylor School | Chattanooga | TN |
| 56 | 3:31.15 | Melanie Klaren (11), Rheanna Vaughn (12), Camille Merwin (12), Stephanie Schlobohm (11) | Aliso Niguel High School | Aliso Viejo | CA |
| 57 | 3:31.36 | Jillian Cordes (12), Kelly Hagerty (12), Anna Veselovsky (10), Olivia Smoliga (9 ) | Glenbrook South High School | Glenview | IL |
| 58 | 3:31.39 | Hannah Molden (12), Samantha Schurig (9 ), Molly Christensen (10), Haley Molden (10) | Washburn Rural High School | Topeka | KS |
| 59 | 3:31.41 | Anna Leonard (10), Sara Snow (12), Elizabeth Mace (12), Olivia Harrell (12) | Glendale High School | Springfield | MO |
| 59 | 3:31.41 | Jennifer Gavin (12), Christina Papadopoulos (10), Gabriella Gil (10), Riki Bonnema (10) | Olympia High School | Orlando | FL |
| 59 | 3:31.41 | Ginny Grams (11), Emily Grams (11), Kendal Casey (10), Shaun Casey (11) | Spruce Creek High School | Port Orange | FL |
| 62 | 3:31.55 | Elise Borja (12), Kayla Skenandore (10), Courtney Sheehan (11), Pamela Breitrick (11) | Waukesha South High School | Waukesha | WI |
| 63 | 3:31.57 | Monica Garcia (12), Charlotte Ruby (10), Haley Oosterhouse (10), Eva Chen (11) | Archbishop Mitty High School | San Jose | CA |
| 64 | 3:31.58 | Emily Faas (9 ), Morgan Emter (10), Cari Caprio (12), Danielle Jansen (12) | Mason High School | Mason | OH |
| 65 | 3:31.66 | Jackie Hodges (11), Lindsay Morrow (9 ), Lauren Sutton (11), Stefania Rossi (11) | Cinco Ranch High School | Katy | TX |
| 66 | 3:31.72 | Kristen Worthington (10), Jennifer Coady (10), Meredith Sweeney (12), Sara Shaner (12) | Hinsdale Central High School | Hinsdale | IL |
| 67 | 3:31.73 | Kate Dillione (11), Bizzy Millen (11), Madison Meyer (10), Maggie Dillione (12) | Council Rock High School | Newtown | PA |
| 68 | 3:31.75 | Marissa LaFreniere (9 ), Keelia Houston (11), Tara Halsted (9 ), Anji Shakya (12) | Davis Senior High School | Davis | CA |
| 69 | 3:31.78 | Sarah Kemp (12), Andrea Laedtke (11), Madeleine Eden (9 ), Nikki Larson (10) | Edina High School | Edina | MN |
| 70 | 3:31.82 | Fawn Weihl (12), Karen Illg (11), Emma O'Connell (11), Elizabeth McGinn (11) | Millard West High School | Omaha | NE |
| 71 | 3:31.89 | Cassidy Lapp (11), Jennifer Hall (9 ), Taylor Cole (12), Claire Singley (12) | Southlake Carroll High School | Southlake | TX |
| 72 | 3:31.99 | Dani Barbiea (11), Ashley Whiteley (9 ), Jennifer Garzinski (11), Kellie Barbiea (11) | Springboro High School | Springboro | OH |
| 73 | 3:32.01 | Courtney McCadden (11), Elsa Jaques (9 ), Rosemary Garcia (10), Gretchen Jaques (11) | Glendora High School | Glendora | CA |
| 74 | 3:32.03 | Morgan Karetnick (10), Erin Calderoni (11), Julie Case (12), Jesse Evans (11) | Greenwich High School | Greenwich | CT |
| 75 | 3:32.04 | Brianna Bentz (11), Hailey Doyle (10), Katie Duggan (11), Mary Moser (12) | Wyomissing Area High School | Wyomissing | PA |
| 76 | 3:32.14 | Kaylee Kucera (10), Rebecca Lauer (11), Abbey Machtemes (12), Audrey Kumm (11) | Hutchinson High School | Hutchinson | MN |
| 77 | 3:32.15 | Jordan Hancock (9 ), Colleen Lord (9 ), Kyle Krueger (12), Romy Landeck (10) | William P. Clements High School | Sugar Land | TX |
| 78 | 3:32.17 | Shane Knight (9 ), Kasey Fontaine (10), Mikkel-Ane Stipe (12), Kendyl Stewart (10) | La Costa Canyon High School | Carlsbad | CA |
| 79 | 3:32.38 | Karima Chuk (12), Briana Wilford (12), Claire Houlton (10), Kirsten Hocheder (12) | Catalina Foothills High School | Tucson | AZ |
| 80 | 3:32.39 | Britta Sortland (11), Cassie Cedarholm (11), Natalie Lugg (12), Elizabeth Gross (12) | Woodbury High School | Woodbury | MN |
| 81 | 3:32.45 | Rachel Astles (10), Katherine Kerman (10), Lilly Nunnally (9 ), Nicole Stafford (9 ) | Westminster Schools | Atlanta | GA |
| 82 | 3:32.47 | Meredith Colombo (12), Brianna Bilunas (11), Grace Raynor (12), Alexandra Bilunas (11) | Duchesne Academy | Omaha | NE |
| 83 | 3:32.50 | Christina Bechtel (11), Alia Welsh (11), Caroline Bechtel (11), Nancy Claire Smith (9 ) | Franklin High School | Franklin | TN |
| 83 | 3:32.50 | Alexa Morris (11), Macartney Welborn (11), Coralyn Gary (11), Colleen Konetzke (10) | Ronald Reagan High School | San Antonio | TX |
| 85 | 3:32.60 | Hannah Lucas (10), Alissa Endres (12), Cati Beecher (11), Jackie Powell (12) | Madison Memorial High School | Madison | WI |
| 86 | 3:32.62 | Christina Vu (9 ), Tiffany Hsu (12), Emily Yu (11), Andrea Grant (11) | Walnut High School | Walnut | CA |
| 87 | 3:32.73 | Brittany Kamper (9 ), Kimberly Katner (9 ), Michelle Kuyper (12), Tara Capouch (10) | Carl Sandburg High School | Orland Park | IL |
| 88 | 3:32.74 | Christy Wilde (9 ), Courtney Wilde (11), Julia Crowley (11), Megan Barnett (11) | Laguna Hills High School | Laguna Hills | CA |
| 89 | 3:32.77 | Allison Viverette (11), Megan Collins (12), Aileen Smith (12), Leah Smith (9 ) | Oakland Catholic High School | Pittsburgh | PA |
| 90 | 3:32.88 | Paige Kostishak (11), Molly Shanley (11), Mari Isa (12), Hanna Cowley (11) | Ann Arbor Pioneer High School | Ann Arbor | MI |
| 91 | 3:32.89 | Casey Duckworth (9 ), Madison McLaren (10), Katie Jackson (12), Kate Klippert (12) | Newport Harbor High School | Newport Beach | CA |
| 92 | 3:32.92 | Maggie Hanson (12), Nina Morrison (9 ), Hayley Good (11), Samantha Asencio (11) | William S. Hart High School | Newhall | CA |
| 93 | 3:32.99 | Mallory McKeon (10), Rebecca Aiello (11), Brittany Hammond (9 ), Siena DeVenuto (12) | Pine Crest School | Fort Lauderdale | FL |
| 94 | 3:33.09 | Megan Nolet (12), Christine La (12), Elizabeth Murray (12), Lauren Opatrny (12) | Saratoga High School | Saratoga | CA |
| 95 | 3:33.10 | Jesse Bergman (12), Anne Peanasky (12), Michelle Wong (9 ), Kristen Clarno (10) | Homestead High School | Cupertino | CA |
| 96 | 3:33.12 | Olivia Samoray (10), Emily Reamer (12), Maria Bargardi (11), Nikki Barczak (11) | Mercy High School | Livonia | MI |
| 97 | 3:33.14 | Kiley Neushul (11), Janelle Nguyen (9 ), Michelle Dockendorf (12), Emily Rose Williams (11) | Dos Pueblos High School | Goleta | CA |
| 98 | 3:33.23 | Carolyn Stewart (11), Carlie Thieman (12), Brittany Morguelan (11), Michelle Gasior (11) | Assumption High School | Louisville | KY |
| 98 | 3:33.23 | Megan Corcoran (12), Sarah Westley (9 ), Bethany DeWitt (10), Mia Barone (12) | West Chester Bayard Rustin High School | West Chester | PA |
| 100 | 3:33.26 | Natalie Morris (11), Mackenzie Neeley (9 ), Mackenna Rife (10), Maddy Dalcamo (12) | Olathe Northwest High School | Olathe | KS |
| 101 | 3:33.27 | Mariah Hamil (10), Raelynn Chastain (9 ), Emilee Duquette (10), Sarah Lohman (10) | Homestead High School | Fort Wayne | IN |
| 102 | 3:33.28 | Lauren Torres (11), Taylor Newgaard (12), Tinsley Andrews (12), Katie Olsen (11) | Desert Vista High School | Phoenix | AZ |
| 103 | 3:33.30 | Liz Saint (11), Lida Walsh (10), Caitlin Bell (10), Julia Treible (12) | Centennial High School | Roswell | GA |
| 104 | 3:33.36 | Catherine Mulquin (9 ), Anna Kolonowski (9 ), Jordan Martinko (9 ), Sarah Haase (10) | Our Lady of Good Counsel | Olney | MD |
| 105 | 3:33.40 | Kelly Dunn (11), Caitlyn Madsen (12), Maria Sedjo (12), Yvette Kruk (9 ) | West Chicago Community High School | West Chicago | IL |
| 105 | 3:33.40 | Michelle Huang (12), Megan Moddy (10), Natalie Parker (10), Lauren Davis (11) | Westwood High School | Austin | TX |
| 107 | 3:33.44 | Maya Owens (10), McKenzie Calkins (11), Holly Moon (11), Danielle Spradlin (11) | Judson High School | Converse | TX |
| 108 | 3:33.48 | Lauren Fitzgerald (9 ), Sophie Weber (10), Hannah Calton (9 ), Quincy Christian (12) | Granite Bay High School | Granite Bay | CA |
| 109 | 3:33.50 | Jenna Harris (10), Kimberli Boone (11), Roslyn Kackman (9 ), Chelsea Weedman (12) | Cathedral Catholic High School | San Diego | CA |
| 110 | 3:33.53 | Melissa Bates (12), Jessica DelMonaco (10), Kathleen Gore (11), Jiana Vargem (9 ) | Mater Dei High School | Santa Ana | CA |
| 111 | 3:33.55 | Natalie Wong (11), Kirsten Brand (9 ), Tory Houston (11), Catherine Breed (11) | Amador Valley High School | Pleasanton | CA |
| 112 | 3:33.57 | Sarah Thompson (11), Amanda Weidner (10), Kristina Walsh (12), Jane Munro (12) | Evanston Township High School | Evanston | IL |
| 113 | 3:33.58 | Nikki Flynn (10), Maddy Frost (9 ), Stephanie Boyle (11), Jordan Rucinski (11) | Saline High School | Saline | MI |
| 114 | 3:33.59 | Katie Miller (9 ), Jennifer Harris (12), Jillian Stoneburg (10), Kathleen Campbell (10) | Akron Firestone High School | Akron | OH |
| 115 | 3:33.62 | Emily Brown (10), Sarah Soltis (12), Kirsten Soucek (12), Hillary Petersen (12) | Buchholz High School | Gainesville | FL |
| 116 | 3:33.71 | Samantha Harding (10), Moranda Madero (10), Jorgi Hobson (12), Rachel Dixon (12) | Las Cruces High School | Las Cruces | NM |
| 117 | 3:33.73 | Meredith Ketchmark (12), Cori Michibata (10), Jocelyn Yuen (10), Rebecca Lewinson (12) | West Windsor-Plainsboro High School South | Princeton Junction | NJ |
| 118 | 3:33.76 | Elizabeth Gelb (11), Annie Tamblyn (10), Allison Rapundalo (11), Anna DeMonte (10) | Ann Arbor Huron High School | Ann Arbor | MI |
| 119 | 3:33.78 | Kate McNeilis (12), Caitlin Harty (12), Laura Cornette (9 ), Elizabeth Harty (9 ) | Dublin Scioto High School | Dublin | OH |
| 120 | 3:33.85 | Nina Zook (11), Meghan O'Keefe (10), Maria Volodkevich (9 ), Andie Taylor (12) | Skyline High School | Sammamish | WA |
| 121 | 3:33.87 | Emma Smith (10), Hayley Krzeczowski (12), Ashley Shanel (10), Kayla Scott (12) | St. Charles East High School | St. Charles | IL |
| 122 | 3:33.90 | Rebecca DeLaFuente (11), Anna Fetter (11), Meredith Smith (12), Suzanne Lemberg (12) | Westfield High School | Westfield | NJ |
| 123 | 3:33.92 | Kate Hellie (12), Molly Peichel (10), Ellen Bloom (9 ), Marisa Wood (9 ) | Sartell High School | Sartell | MN |
| 124 | 3:33.95 | Katya Borisov (9 ), Stephanie Graham (12), Courtney Thompson (12), Kally Vanderbilt (10) | North Allegheny Senior High School | Wexford | PA |
| 124 | 3:33.95 | Emily Schwartz (12), Maryn Bieganski (12), Melissa Besman (12), Annie Marquiss (11) | Solon High School | Solon | OH |
| 126 | 3:33.98 | Emily Wisolek (12), Annie Cohoat (12), Jillian Welch (12), Sarah Griffin (11) | Northridge High School | Middlebury | IN |
| 127 | 3:34.03 | Danielle Kerr (12), Hannah Bunkin (10), Kathryn Clare-Salzler (10), Christine Kerr (12) | Eastside High School | Gainesville | FL |
| 128 | 3:34.06 | Samantha Zuch (12), Jesse Sworden (10), Chandler Ashbaugh (10), Erika Vocke (10) | Napoleon High School | Napoleon | OH |
| 129 | 3:34.08 | Stephanie Nguyen (12), Roshini Hegde (12), Julia Hu (12), Kate Yu (10) | Lynbrook High School | San Jose | CA |
| 130 | 3:34.10 | Melanie McClure (12), Lauren Bennett (10), Sooyeon Kim (11), Meagan Thompson (9 ) | Mansfield High School | Mansfield | TX |