| Place | Time | Swimmer (Yr) | School | City | State |
| 1 | :51.53 | Rachel Bootsma (11) | Eden Prairie High School | Eden Prairie | MN |
| 2 | :52.18 | * Missy Franklin (10) | Regis Jesuit High School | Aurora | CO |
| * Note - Missy Franklin's :52.18 was altituded adjusted - her unadjusted time is :52.30 and the current NISCA Independent National Record | |||||
| 3 | :52.45 | Courtney Bartholomew (11) | Holland High School | Holland | MI |
| 4 | :53.23 | Melanie Klaren (12) | Aliso Niguel High School | Aliso Viejo | CA |
| 5 | :53.31 | Ally Howe (9 ) | Sacred Heart Preparatory | Atherton | CA |
| 6 | :53.43 | Olivia Smoliga (10) | Glenbrook South High School | Glenview | IL |
| 7 | :53.54 | Jillian Vitarius (11) | Kingwood High School | Kingwood | TX |
| 8 | :53.64 | Engel Lindsey (9 ) | Crean Lutheran High School | Irvine | CA |
| 9 | :53.74 | Madison White (10) | Carondelet High School | Concord | CA |
| 10 | :53.82 | Annemarie Thayer (12) | Ballard High School | Seattle | WA |
| 11 | :53.89 | Arden Pitman (12) | The Baylor School | Chattanooga | TN |
| 12 | :54.15 | Caitlyn Forman (11) | Notre Dame Academy | Park Hills | KY |
| 13 | :54.26 | Lacey Locke (10) | Carmel High School | Carmel | IN |
| 14 | :54.27 | Rachel Moore (11) | Andover High School | Andover | MA |
| 15 | :54.45 | Sidney Cooke (10) | Monte Vista High School | Danville | CA |
| 16 | :54.61 | Kendyl Stewart (11) | La Costa Canyon High School | Carlsbad | CA |
| 17 | :54.73 | Taylor Sneed (12) | Germantown Academy | Fort Washington | PA |
| 18 | :54.76 | Sabrina Benson (12) | Broughton High School | Raleigh | NC |
| 18 | :54.76 | Clara Smiddy (9 ) | South Florida Heat | Miami | FL |
| 20 | :54.77 | Christina E. Leander (10) | West Chester East High School | West Chester | PA |
| 21 | :54.84 | Melanie Busch (12) | William Tennent High School | Warminster | PA |
| 22 | :54.93 | Hannah Magnuson (9 ) | Christian Academy Of Louisville | Louisville | KY |
| 22 | :54.93 | Allie Roberts (12) | Emmaus High School | Emmaus | PA |
| 24 | :54.95 | Lauren Harrington (12) | St. Mary's Episcopal School | Memphis | TN |
| 25 | :55.06 | Alexis Bullard (11) | Carmel High School | Carmel | IN |
| 25 | :55.06 | Yurie Nakano (12) | Hershey High School | Hershey | PA |
| 27 | :55.13 | Sydney Johansen (12) | St. Francis High School | Sacramento | CA |
| 28 | :55.15 | Rebekah Land (12) | Hannibal High School | Hannibal | MO |
| 29 | :55.21 | Erica Belcher (12) | Reading Memorial High School | Reading | MA |
| 30 | :55.24 | Haley Preston (11) | Westlake High School | Westlake Village | CA |
| 31 | :55.25 | Chelsea Britt (10) | Lake Highland Preparatory School | Orlando | FL |
| 32 | :55.26 | Kimberlee John-Williams (10) | The Baylor School | Chattanooga | TN |
| 33 | :55.29 | Genny Pittman (9 ) | Homewood High School | Homewood | AL |
| 34 | :55.34 | Marie Georger (11) | Mercyhurst Preparatory School | Erie | PA |
| 34 | :55.34 | Kaitlyn Jones (10) | The Tatnall School | Wilmington | DE |
| 34 | :55.34 | Sophie Weber (11) | Granite Bay High School | Granite Bay | CA |
| 37 | :55.37 | Marissa Cominelli (10) | Hawken School | Gates Mills | OH |
| 38 | :55.40 | Alexandra DeLoof (11) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 39 | :55.46 | Maggie Gruber (10) | Mars Area High School | Mars | PA |
| 40 | :55.47 | Margaret Cooke (10) | The Woodlands High School | The Woodlands | TX |
| 41 | :55.48 | Samantha Bosma (12) | Lassiter High School | Marietta | GA |
| 41 | :55.48 | Ashleigh Ferguson (11) | Academy Of The Holy Cross | Kensington | MD |
| 43 | :55.60 | Janet Hu (9 ) | Oakton High School | Vienna | VA |
| 43 | :55.60 | Arlyn Upshaw (11) | Piedmont High School | Piedmont | CA |
| 45 | :55.63 | Anna Mayfield (11) | Smithson Valley High School | Spring Branch | TX |
| 46 | :55.65 | Allie Sullins (11) | Magnolia West High School | Magnolia | TX |
| 47 | :55.72 | Lauren Damian (12) | Monte Vista High School | Danville | CA |
| 48 | :55.76 | Claudia Doyle (12) | Thomas Worthington High School | Worthington | OH |
| 48 | :55.76 | Breanna Tran (9 ) | Beckman High School | Irvine | CA |
| 50 | :55.77 | Stephanie Schlobohm (11) | Aliso Niguel High School | Aliso Viejo | CA |
| 51 | :55.78 | Hanna House (9 ) | Homestead High School | Fort Wayne | IN |
| 52 | :55.79 | Shirley Wang (12) | Plano Senior High School | Plano | TX |
| 53 | :55.82 | Gabby Sims (9 ) | Downers Grove North High School | Downers Grove | IL |
| 54 | :55.84 | Gia Dalesandro (10) | Neuqua Valley High School | Naperville | IL |
| 55 | :55.87 | Maddie Bro (11) | Ames High School | Ames | IA |
| 55 | :55.87 | Emma Wyke (10) | Columbus North High School | Columbus | IN |
| 57 | :55.89 | Ashlee Linn (11) | Riverview High School | Sarasota | FL |
| 58 | :55.90 | Kathryn Eckhart (11) | Cy Creek High School | Houston | TX |
| 59 | :55.91 | Alison Meng (12) | Sauk Prairie High School | Prairie du Sac | WI |
| 60 | :55.92 | Anna Labozan (12) | Monarch High School | Louisville | CO |
| 61 | :55.94 | Tasija Karosas (10) | Saint Andrew's School | Boca Raton | FL |
| 62 | :55.96 | Douglas Kayla (12) | Livonia Franklin High School | Livonia | MI |
| 62 | :55.96 | Shaya Schaedler (11) | Campbell County High School | Gillette | WY |
| 64 | :56.02 | Kenzie Miller (12) | Plymouth High School | Plymouth | IN |
| 64 | :56.02 | Katie Miller (10) | Akron Firestone High School | Akron | OH |
| 66 | :56.06 | Megan McCarroll (12) | Jesuit High School | Portland | OR |
| 67 | :56.07 | Rebecca Baxley (10) | Analy High School | Sebastopol | CA |
| 67 | :56.07 | Andrea Vandermey (10) | Novi High School | Novi | MI |
| 69 | :56.08 | Sarah Lohman (11) | Homestead High School | Fort Wayne | IN |
| 70 | :56.09 | Samantha James (11) | New Trier High School | Winnetka | IL |
| 70 | :56.09 | Emma Szczupakiewicz (11) | Muskego High School | Muskego | WI |
| 72 | :56.11 | Camey Rabold (11) | Bishop Watterson High School | Columbus | OH |
| 73 | :56.13 | Hannah Peiffer (10) | The Baylor School | Chattanooga | TN |
| 73 | :56.13 | Konnor Rist (11) | DuPont Manual High School | Louisville | KY |
| 75 | :56.16 | Larah Beaver (11) | Yorktown High School | Yorktown | IN |
| 76 | :56.20 | Anna Grainger (11) | Ensworth High School | Nashville | TN |
| 77 | :56.21 | Morgan Belli (9 ) | Gahanna Lincoln High School | Gahanna | OH |
| 78 | :56.23 | Kayla Grim (12) | Parkland High School | Allentown | PA |
| 79 | :56.24 | Jenna Bickel (11) | Guilderland Central High School | Guilderland Center | NY |
| 79 | :56.24 | Holly Harper (11) | Albemarle High School | Charlottesville | VA |
| 81 | :56.27 | Betsy Zilch (11) | St. Ursula Academy | Cincinnati | OH |
| 82 | :56.29 | Emily Baturka (11) | Episcopal Academy | Newtown Square | PA |
| 83 | :56.30 | Emma Paulson (10) | Wayzata High School | Plymouth | MN |
| 84 | :56.33 | Tara Halsted (10) | Davis Senior High School | Davis | CA |
| 85 | :56.35 | Spencer Chappell (12) | Northridge High School | Middlebury | IN |
| 85 | :56.35 | Nina Hayes (10) | Granada High School | Livermore | CA |
| 87 | :56.37 | Morgan Fairclough (10) | Monroe Woodbury High School | Central Valley | NY |
| 88 | :56.38 | Gabrielle DeLoof (9 ) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 88 | :56.38 | Devin Madsen (11) | Las Lomas High School | Walnut Creek | CA |
| 90 | :56.41 | Leticia Lelli (11) | Chaparral High School | Scottsdale | AZ |
| 91 | :56.43 | Brooke Waugaman (12) | Hempfield Area High School | Greensburg | PA |
| 92 | :56.48 | Heather Coutts (12) | Bishop Kenny High School | Jacksonville | FL |
| 92 | :56.48 | Alexis Mayhall (12) | Palo Verde High School | Las Vegas | NV |
| 92 | :56.48 | Kendese Nangle (11) | The Peddie School | Hightstown | NJ |
| 92 | :56.48 | Carolyn Stewart (12) | Assumption High School | Louisville | KY |
| 96 | :56.49 | Talie Anchustegui (11) | Xavier College Preparatory | Phoenix | AZ |
| 97 | :56.50 | Kate Jones (11) | Arrowhead Union High School | Hartland | WI |
| 98 | :56.52 | Cheyenne Low (10) | University High School | Irvine | CA |
| 99 | :56.54 | Caroline Page (10) | Pace High School | Pace | FL |
| 99 | :56.54 | Angela Sun (11) | Great Neck South High School | Great Neck | NY |