| Place | Time | Swimmer (Yr) | School | City | State |
| 1 | :48.64 | Maddy Schaefer (12) | Saint Francis High School | Mountain View | CA |
| 2 | :48.76 | Kristen Vredeveld (10) | The Baylor School | Chattanooga | TN |
| 3 | :49.03 | Rachael Acker (11) | Gunn High School | Palo Alto | CA |
| 4 | :49.24 | Madeline Locus (12) | Dulles High School | Sugar Land | TX |
| 4 | :49.24 | Lindsey McKnight (11) | American Heritage School | Plantation | FL |
| 6 | :49.32 | Meaghan Raab (9 ) | Hershey High School | Hershey | PA |
| 7 | :49.37 | Engel Lindsey (9 ) | Crean Lutheran High School | Irvine | CA |
| 7 | :49.37 | Danielle Siverling (12) | Downingtown East High School | Exton | PA |
| 9 | :49.45 | Emily Cameron (10) | Warwick High School | Lititz | PA |
| 9 | :49.45 | Emily Dicus (12) | Topeka West High School | Topeka | KS |
| 11 | :49.50 | Bonnie Brandon (11) | Cherry Creek High School | Greenwood Village | CO |
| 12 | :49.69 | Julia Anderson (11) | R. L. Paschal High School | Fort Worth | TX |
| 12 | :49.69 | Catherine Breed (12) | Amador Valley High School | Pleasanton | CA |
| 14 | :49.77 | Kendyl Stewart (11) | La Costa Canyon High School | Carlsbad | CA |
| 15 | :49.89 | Aja Van Hout (12) | Madison East High School | Madison | WI |
| 16 | :49.94 | Hannah Bowen (12) | Stillwater Area High School | Stillwater | MN |
| 17 | :50.07 | Caroline Piehl (12) | Smoky Hill High School | Aurora | CO |
| 18 | :50.16 | Lauren Driscoll (11) | American Heritage School | Plantation | FL |
| 19 | :50.19 | Dani Barbiea (12) | Springboro High School | Springboro | OH |
| 20 | :50.22 | Rhiannon Sheets (12) | Carmel High School | Carmel | IN |
| 21 | :50.27 | Nikki Barczak (12) | Mercy High School | Farmington Hills | MI |
| 22 | :50.28 | Kaitlyn Dressel (12) | Clay High School | Green Cove Springs | FL |
| 23 | :50.31 | Samantha Bosma (12) | Lassiter High School | Marietta | GA |
| 23 | :50.31 | Janet Hu (9 ) | Oakton High School | Vienna | VA |
| 23 | :50.31 | Faith Johnson (11) | High Point Christian Academy | High Point | NC |
| 26 | :50.32 | Julia Ama (11) | Gunn High School | Palo Alto | CA |
| 27 | :50.36 | Casey Clark (12) | Klein High School | Spring | TX |
| 28 | :50.37 | Rachel Moore (11) | Andover High School | Andover | MA |
| 29 | :50.38 | Zoe Mattingly (11) | Zionsville Community High School | Zionsville | IN |
| 30 | :50.40 | Ally Hardesty (11) | Bearden High School | Knoxville | TN |
| 31 | :50.46 | Annemarie Thayer (12) | Ballard High School | Seattle | WA |
| 32 | :50.48 | Ally Howe (9 ) | Sacred Heart Preparatory | Atherton | CA |
| 33 | :50.49 | Madison White (10) | Carondelet High School | Concord | CA |
| 34 | :50.51 | Meredith Oliver (11) | Robert E. Lee High School | Tyler | TX |
| 35 | :50.52 | Chandra Lukes (12) | San Pasqual High School | Escondido | CA |
| 36 | :50.53 | Linnea Mack (10) | Pioneer High School | San Jose | CA |
| 36 | :50.53 | Caroline Thomas (12) | DuPont Manual High School | Louisville | KY |
| 38 | :50.56 | Heidi Busack (12) | Fergus Falls High School | Fergus Falls | MN |
| 39 | :50.57 | Lauren Harrington (12) | St. Mary's Episcopal School | Memphis | TN |
| 40 | :50.66 | Katherine Huff (11) | Lakeside High School | Evans | GA |
| 41 | :50.69 | Henriette Stenkvist (11) | Panther Creek High School | Cary | NC |
| 42 | :50.70 | Samantha James (11) | New Trier High School | Winnetka | IL |
| 43 | :50.75 | Cheyenne Low (10) | University High School | Irvine | CA |
| 43 | :50.75 | Carly Marshall (12) | Carmel High School | Carmel | IN |
| 45 | :50.80 | Hanna Cowley (12) | Ann Arbor Pioneer High School | Ann Arbor | MI |
| 46 | :50.82 | Savannah Miller (11) | Westlake High School | Westlake Village | CA |
| 47 | :50.86 | Madison Martin (10) | Pickerington High School Central | Pickerington | OH |
| 48 | :50.88 | Alyson Viles (12) | Taft High School | Lincoln City | OR |
| 49 | :50.90 | Suzanne Bessire (12) | Lake Highland Preparatory School | Orlando | FL |
| 50 | :50.91 | Jamie Friderichs (11) | Kingwood High School | Kingwood | TX |
| 50 | :50.91 | Olivia Kabacinski (11) | Chesterton High School | Chesterton | IN |
| 52 | :50.95 | Nikki Larson (11) | Edina High School | Edina | MN |
| 53 | :50.96 | Amber Cratsenberg (11) | Thomas Jefferson High School | Auburn | WA |
| 54 | :50.97 | Jenny Darby (12) | Hardin Valley Academy | Knoxville | TN |
| 55 | :51.00 | Ashlee Linn (11) | Riverview High School | Sarasota | FL |
| 56 | :51.01 | Elizabeth McDonald (11) | Pomperaug High School | Southbury | CT |
| 57 | :51.03 | Annie Tamblyn (11) | Ann Arbor Huron High School | Ann Arbor | MI |
| 58 | :51.06 | Andrea Grant (12) | Walnut High School | Walnut | CA |
| 59 | :51.08 | Nancy Larson (12) | Mount Tabor High School | Winston-Salem | NC |
| 59 | :51.08 | Gabby Sims (9 ) | Downers Grove North High School | Downers Grove | IL |
| 61 | :51.11 | Kristen Clarno (11) | Homestead High School | Cupertino | CA |
| 61 | :51.11 | Dana Holt (12) | Campolindo High School | Moraga | CA |
| 61 | :51.11 | Mary Schneider (9 ) | Fenwick High School | Oak Park | IL |
| 64 | :51.12 | Sammie Hashbarger (9 ) | McKinney High School | McKinney | TX |
| 64 | :51.12 | Hillary Thomas (12) | Regis Jesuit High School | Aurora | CO |
| 66 | :51.16 | Morgan Cohara (11) | Hawken School | Gates Mills | OH |
| 66 | :51.16 | Alexa Morris (12) | Ronald Reagan High School | San Antonio | TX |
| 66 | :51.16 | Kat Simonovic (10) | Mountain View High School | Mesa | AZ |
| 69 | :51.17 | Carly Reid (9 ) | Monta Vista High School | Cupertino | CA |
| 70 | :51.18 | Emily Ryczek (12) | Sherwood High School | Sandy Spring | MD |
| 71 | :51.19 | Caitlyn Karr (12) | Wilson High School | West Lawn | PA |
| 72 | :51.23 | Carolyn Fittin (12) | The Peddie School | Hightstown | NJ |
| 72 | :51.23 | Susanne Gingher (12) | State College Area High School | State College | PA |
| 74 | :51.24 | Katheryne Muth (12) | Sarasota High School | Sarasota | FL |
| 74 | :51.24 | Emily Stang (11) | Parkland High School | Allentown | PA |
| 76 | :51.25 | Anika Apostalon (10) | Albuquerque Academy | Albuquerque | NM |
| 76 | :51.25 | Sami Pochowski (11) | Muskego High School | Muskego | WI |
| 78 | :51.26 | Heather Arseth (12) | Wayzata High School | Plymouth | MN |
| 78 | :51.26 | Alexandra Marshall (10) | Ronald Wilson Reagan High School. | Pfafftown | NC |
| 80 | :51.27 | Olivia Boggs (12) | Woodward Academy | College Park | GA |
| 80 | :51.27 | Taylor Carlson (12) | Las Lomas High School | Walnut Creek | CA |
| 80 | :51.27 | Katie Diller (10) | Manheim Township High School | Lancaster | PA |
| 80 | :51.27 | Kayla Hammerberg (12) | Upper Arlington High School | Upper Arlington | OH |
| 84 | :51.28 | Anna Labozan (12) | Monarch High School | Louisville | CO |
| 85 | :51.30 | Ginny Grams (12) | Spruce Creek High School | Port Orange | FL |
| 85 | :51.30 | Sarah Koucheki (10) | Hawken School | Gates Mills | OH |
| 87 | :51.31 | Ellen Williamson (12) | Notre Dame Academy | Park Hills | KY |
| 87 | :51.31 | Kina Zhou (10) | Niskayuna High School | Niskayuna | NY |
| 89 | :51.34 | Haley Pietila (11) | Arrowhead Union High School | Hartland | WI |
| 90 | :51.36 | Zoe Judd (12) | Acton-Boxboro Regional High School | Acton | MA |
| 91 | :51.39 | Mackenzie Schuler (12) | Clovis West High School | Fresno | CA |
| 91 | :51.39 | Kelsi Worrell (11) | Rancocas Valley High School | Mt. Holly | NJ |
| 93 | :51.40 | Alexandra DeLoof (11) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 93 | :51.40 | Helena Gali (12) | Los Gatos High School | Los Gatos | CA |
| 95 | :51.41 | Alisha Bender (11) | Xavier College Preparatory | Phoenix | AZ |
| 95 | :51.41 | Macie McNichols (9 ) | Gilmour Academy | Gates Mills | OH |
| 95 | :51.41 | Haley Sims (12) | Downers Grove North High School | Downers Grove | IL |
| 98 | :51.42 | Allison Brown (11) | San Ramon Valley High School | Danville | CA |
| 98 | :51.42 | Mariah Hamil (11) | Homestead High School | Fort Wayne | IN |
| 98 | :51.42 | Elaina Lanson (12) | Wadsworth City Schools | Wadsworth | OH |