| Place | Time | Swimmers (Yrs) | School | City | State |
| 1 | 1:32.63 | Kristen Vredeveld (10), Ashley Yearwood (11), Emma Michaels (12), Arden Pitman (12) | The Baylor School | Chattanooga | TN |
| 2 | 1:34.12 | Gabrielle Veri (11), Kayla Hammerberg (12), Lauren Comer (11), Abigail Chin (12) | Upper Arlington High School | Upper Arlington | OH |
| 3 | 1:34.14 | Carly Marshall (12), Lucy Kramer (11), Rhiannon Sheets (12), Lacey Locke (10) | Carmel High School | Carmel | IN |
| 4 | 1:34.16 | Carolyn Fittin (12), Molly Mucciarone (11), Jillian Liwacz (12), Kendese Nangle (11) | The Peddie School | Hightstown | NJ |
| 5 | 1:34.23 | Meaghan Raab (9 ), Madelyn Veith (9 ), Alexis Grier (11), Katelyn Miller (12) | Hershey High School | Hershey | PA |
| 6 | 1:34.40 | Jordan Mattern (12), Fiona Kane (11), Elaine Powell (10), Bonnie Brandon (11) | Cherry Creek High School | Greenwood Village | CO |
| 7 | 1:34.49 | Alexandra Rockett (9 ), Taylor Straub (12), Camilla Czulada (11), Caitlyn Karr (12) | Wilson High School | West Lawn | PA |
| 8 | 1:34.50 | Margaret Wenzlau (12), Jayna Wittenbrink (11), Sarah Liang (12), Jasmine Tosky (11) | Palo Alto High School | Palo Alto | CA |
| 9 | 1:34.59 | Meagan Dollard (9 ), Natalie Dowzicky (9 ), Rachel Zilinskas (10), Sarah Aemisegger (12) | Germantown Academy | Fort Washington | PA |
| 10 | 1:34.86 | Mary Warren (9 ), Holly Johnson (12), Katie Duranso (10), Haley Pietila (11) | Arrowhead Union High School | Hartland | WI |
| 11 | 1:34.94 | Alexa Morris (12), Sarah Gibson (10), Colleen Konetzke (11), Coralyn Gary (12) | Ronald Reagan High School | San Antonio | TX |
| 12 | 1:34.96 | Ivy Martin (12), Maddie Martin (9 ), Chelsea Soderholm (11), Aja Van Hout (12) | Madison East High School | Madison | WI |
| 13 | 1:35.03 | Camey Rabold (11), Aly Francis (11), Michele Rielly (11), Samantha Schuttinger (10) | Bishop Watterson High School | Columbus | OH |
| 14 | 1:35.10 | Mariah Hamil (11), Chelsey Reber (11), Whitney Carroll (9 ), Tori Bagan (12) | Homestead High School | Fort Wayne | IN |
| 15 | 1:35.21 | Sarah Graif (12), Alisha Bender (11), Talie Anchustegui (11), Kayra Zsiga (12) | Xavier College Preparatory | Phoenix | AZ |
| 16 | 1:35.32 | Taylor Steffl (12), Elliott Schinella (9 ), Sato Kakihara (11), Nikki Barczak (12) | Mercy High School | Farmington Hills | MI |
| 17 | 1:35.40 | Sarah Love (10), Sarah Barker (11), Emily Holt (11), Caroline Thomas (12) | DuPont Manual High School | Louisville | KY |
| 18 | 1:35.54 | Alexis Walley (12), Erica Reifinger (11), Megan Polaha (10), Emily Stang (11) | Parkland High School | Allentown | PA |
| 19 | 1:35.61 | Emma Henderson (10), Erin Parrett (11), Megan Rankin (11), Maggie Burton (10) | University High School | Irvine | CA |
| 20 | 1:35.67 | Lindsay Morrow (10), Lauren Sutton (12), Stefania Rossi (12), Jackie Hodges (12) | Cinco Ranch High School | Katy | TX |
| 21 | 1:35.71 | Emily Baturka (11), Bernadette Tankle (9 ), Lauren Decker (11), Tara O'Prey (10) | Episcopal Academy | Newtown Square | PA |
| 22 | 1:35.77 | Michaela Rookus (11), Audra Thornton (11), Makayla Myers (9 ), Danielle Freeman (10) | Hudsonville High School | Hudsonville | MI |
| 23 | 1:35.81 | Sarah Koucheki (10), Mackenzie Harris (11), Caroline Vexler (10), Morgan Cohara (11) | Hawken School | Gates Mills | OH |
| 24 | 1:35.83 | Kaitlyn Ruffing (10), Alyssa Ruffing (9 ), Mackenzie Meixner (11), Aryn Zimmerman (12) | Franklin Regional High School | Murrysville | PA |
| 25 | 1:35.85 | Maddy Schaefer (12), Courtney Batcheller (9 ), Stefanie Scherp (10), Jessie Ho (10) | Saint Francis High School | Mountain View | CA |
| 26 | 1:35.98 | Rachel Burke (11), Amber Calderone (10), Sarah Sykstus (11), Katherine Hare (11) | Rosary High School | Aurora | IL |
| 27 | 1:36.07 | Cassidy Lapp (12), Kaitlyn Hogan (12), Lyndsie Gibson (11), Taylor Derevyanik (11) | Southlake Carroll High School | Southlake | TX |
| 27 | 1:36.07 | Heather Arseth (12), Laura Peterson (12), Kelly Caprio (12), Sarah Deis (12) | Wayzata High School | Plymouth | MN |
| 29 | 1:36.11 | Courtney Wilde (12), Julia Crowley (12), Megan Barnett (12), Christy Wilde (10) | Laguna Hills High School | Laguna Hills | CA |
| 30 | 1:36.23 | Kate Dillione (12), Bizzy Millen (12), Tommie Dillione (9 ), Madison Meyer (11) | Council Rock North High School | Newtown | PA |
| 31 | 1:36.25 | Rachael Acker (11), Julia Ama (11), Casey Lincoln (11), Gabrielle Bethke (9 ) | Gunn High School | Palo Alto | CA |
| 31 | 1:36.25 | Morgan Scott (11), Ashleigh Stoddart (12), Elizabeth Good (11), Megan Marchuk (12) | New Trier High School | Winnetka | IL |
| 33 | 1:36.27 | Corinne Caldwell (11), Rachel Green (10), Kacie VanBuskirk (12), Paige Drazga (12) | South Lyon High School | South Lyon | MI |
| 34 | 1:36.29 | Mary Schneider (9 ), Haley Wickham (10), Paulina Kaminski (9 ), Maureen Barron (11) | Fenwick High School | Oak Park | IL |
| 35 | 1:36.31 | Meghan Bicomong (10), Courtney Whyte (12), Sam Flower (10), Dana Holt (12) | Campolindo High School | Moraga | CA |
| 36 | 1:36.32 | Reia Tong (11), Sarah Kannan (9 ), Audrey Gould (10), Charlotte Meyer (10) | Walt Whitman High School | Bethesda | MD |
| 37 | 1:36.35 | Katerina Borisov (10), Arielle Krigger (10), Marina Rozick (12), Kally Vanderbilt (11) | North Allegheny Senior High School | Wexford | PA |
| 37 | 1:36.35 | Lauren Neidigh (12), Savannah Fernandez (11), Samantha Rahael (11), Ashley Neidigh (10) | The Bolles School | Jacksonville | FL |
| 39 | 1:36.38 | Olivia Boggs (12), Ellie Behr (11), Kathleen Cook (9 ), Caitlin Cooper (9 ) | Woodward Academy | College Park | GA |
| 40 | 1:36.45 | Adrienne Hebb (12), Heather Laedtke (10), Madeleine Eden (10), Nikki Larson (11) | Edina High School | Edina | MN |
| 41 | 1:36.51 | Alexandria Sorensen (11), Carly Pratt (9 ), Jennifer Reese (12), Erin Foster (12) | The Woodlands High School | The Woodlands | TX |
| 42 | 1:36.57 | Meghan Overend (12), Jayme Katis (12), Megan Howard (12), Abi Speers (10) | Langley High School | McLean | VA |
| 43 | 1:36.58 | Mallory Remick (11), Maddie Jardeleza (10), Ellie Ansani (12), Evan Swenson (10) | Loyola Academy | Wilmette | IL |
| 44 | 1:36.63 | Emma O'Connell (12), Katherine McGinn (9 ), Faith Daley (12), Elizabeth McGinn (12) | Millard West High School | Omaha | NE |
| 45 | 1:36.65 | Ritchey Howe (11), Liza Bragg (10), Julie Hwang (10), Jenner McLeod (10) | Deerfield Academy | Deerfield | MA |
| 46 | 1:36.70 | Alexis Lee (11), Aislinn Dresel (9 ), Ali Thomason (11), Kimmie Kreuzberger (11) | Marin Catholic High School | Kentfield | CA |
| 47 | 1:36.73 | Marissa Konicke (12), Natalie Sanchez (12), Ashley Ezell (11), Deanna Marks (12) | Plano Senior High School | Plano | TX |
| 48 | 1:36.76 | Jalynn Gieseke (12), Meredith Voss (12), Kaylan Gieseke (10), Rebekah Land (12) | Hannibal High School | Hannibal | MO |
| 49 | 1:36.78 | Jaynie Pulte (11), Clare Nienstedt (9 ), Madison Taylor (10), Molly Schaefer (12) | Marian High School | Bloomfield Hills | MI |
| 50 | 1:36.79 | Alana Arnold (12), Logan Arnold (11), Stephanie Nemeth (11), Rachel Ciferno (11) | Mayfield High School | Mayfield Village | OH |
| 51 | 1:36.82 | Tara Rogers (9 ), Mary Morris (12), Eve Kosten (9 ), Brooke Else (11) | North Penn High School | Lansdale | PA |
| 52 | 1:36.87 | Ginny Grams (12), Emily Grams (12), Shaun Casey (12), Kelsey Hoffman (11) | Spruce Creek High School | Port Orange | FL |
| 53 | 1:36.89 | Christina Ray (11), Nicole Palmer (11), Allison Gargalikis (12), Natalie Amberg (9 ) | Carondelet High School | Concord | CA |
| 54 | 1:36.95 | Alina Schulhofer (10), Rebecca Aiello (12), Stephanie Moeller (12), Brittany Hammond (10) | Pine Crest School | Fort Lauderdale | FL |
| 55 | 1:36.98 | Anna Babinec (10), Lauren DeShaw (10), Elizabeth Fris (10), Shelby Lacy (12) | Holland West Ottawa High School | Holland | MI |
| 56 | 1:36.99 | Ashley Vojtek (11), Natalie Feola (9 ), Shannon Haberman (11), Leah Smith (10) | Oakland Catholic High School | Pittsburgh | PA |
| 57 | 1:37.00 | Shannen Wehrman (12), Cami Luhrs (11), Lisa Swank (12), Leah Pftizer (10) | Kingwood High School | Kingwood | TX |
| 58 | 1:37.01 | Lauren Foor (12), Kate Curley (10), Julia Campbell (10), Olivia Kabacinski (11) | Chesterton High School | Chesterton | IN |
| 58 | 1:37.01 | Kelsey McRill (11), Francesca Viotti (11), Katherine Taylor (12), Annie Marquiss (12) | Solon High School | Solon | OH |
| 60 | 1:37.06 | Zoe Mattingly (11), Alex Cleveland (9 ), Lizzie Lyon (11), Hannah Mitchell (9 ) | Zionsville Community High School | Zionsville | IN |
| 61 | 1:37.10 | Alexandra Marshall (10), Maria Seward (10), Meghan Babcock (12), Lillian Gordy (12) | Ronald Wilson Reagan High School | Pfafftown | NC |
| 62 | 1:37.12 | Alli Gillespie (12), Ellie Morrissey (11), Tori Smith (12), Harley Owens (12) | Edison High School | Huntington Beach | CA |
| 63 | 1:37.14 | Danielle Yoon (10), Shelby Shelton (11), Kelly Denkler (10), Rachel Moody (12) | Memorial High School | Houston | TX |
| 64 | 1:37.21 | Alex Bilunas (12), Breighanne Lawrence (10), Jennelle Jarrett (10), Brianna Bilunas (12) | Duchesne Academy | Omaha | NE |
| 64 | 1:37.21 | Kellie La Mothe (11), Mary Grace Van Allen (10), Alexandra Hofmann (12), Anna Dehnke (11) | North Central High School | Indianapolis | IN |
| 66 | 1:37.23 | Holly Morren (9 ), Klare Northuis (11), Haley Thompson (11), Nicole Cordero (11) | Holland High School | Holland | MI |
| 67 | 1:37.27 | Sydney Gathe (9 ), Megan Childs (10), Courtney Taylor (10), Margaret Maxstadt (11) | Neuqua Valley High School | Naperville | IL |
| 67 | 1:37.27 | Nicole Skaggs (11), Ariel Jordan (10), Eva Pold (11), Margaret Guo (11) | Torrey Pines High School | San Diego | CA |
| 69 | 1:37.31 | Maria Bernard (12), Rebecca Bernard (12), Liz Bales (9 ), Riki Bonnema (11) | Olympia High School | Orlando | FL |
| 69 | 1:37.31 | Emma Camille Siewert (11), Carylyn Waite (9 ), Katie Carter (11), Rebecca Werdine (11) | Tampa Preparatory School | Tampa | FL |
| 71 | 1:37.32 | Jordan Domeier (10), Casey Clark (12), Katelyn Crawford (10), Katelyn Bain (12) | Klein High School | Spring | TX |
| 71 | 1:37.32 | Suzanne Bessire (12), Chelsea Britt (10), Hayley Springman (12), Danielle Salerno (12) | Lake Highland Preparatory School | Orlando | FL |
| 71 | 1:37.32 | Rachel Bernhardt (10), Annemarie Ruberti (11), Allison Humphries (12), Lauren Carastro (12) | Vineland High School | Vineland | NJ |
| 74 | 1:37.33 | Taylor Pfershy (11), Annie Lazor (11), Hanna Pfershy (9 ), Elise Dalley (12) | Birmingham Groves High School | Beverly Hills | MI |
| 74 | 1:37.33 | Erin Schoenfeld (9 ), Megan Howard (10), Shelly Caron (12), Meredith White (12) | Miramonte High School | Orinda | CA |
| 76 | 1:37.35 | Christina Hurley (12), Kathrine Wetherbee (12), Megan Griffin (11), Lauryn Brown (12) | Lincoln Sudbury Regional High School | Sudbury | MA |
| 77 | 1:37.36 | Samantha Miller (11), Megan Hawk (9 ), Laura Duncan (10), Taylor Waggoner (12) | Fremont Ross High School | Fremont | OH |
| 78 | 1:37.37 | Allie Walsh (10), Amber Jenkins (12), Lauren Jones (10), Whitney Allen (11) | Frisco High School | Frisco | TX |
| 78 | 1:37.37 | Lindsey Schmidt (11), Emeline Leyens (10), Ellie Houser (12), Carolyn Meier (11) | Upper Dublin High School | Fort Washington | PA |
| 78 | 1:37.37 | Ellie Hogan (9 ), Lilly Nunnally (10), Erika Redding (10), Rachel Astles (11) | Westminster Schools | Atlanta | GA |
| 81 | 1:37.40 | Nikki Flynn (11), Stephanie Boyle (12), Jordan Rucinski (12), Kate Rogers (9 ) | Saline High School | Saline | MI |
| 82 | 1:37.41 | Tarin Anding (11), Molly Peichel (11), Lauren Martens (10), Marisa Wood (10) | Sartell High School | Sartell | MN |
| 83 | 1:37.50 | Katie Ford (9 ), Courtney Errington (11), Megan Gardner (11), Meghan Schilken (9 ) | Mt. Lebanon High School | Pittsburgh | PA |
| 84 | 1:37.54 | Alex Rieger (9 ), Antoinette Ranit (12), Jessica Poelman (12), Cora Stebbins (11) | St. Francis High School | Sacramento | CA |
| 85 | 1:37.58 | Jaclyn Amog (12), Emily Albaugh (10), Brittany Heng (11), Kelsey Leonard (10) | Las Lomas High School | Walnut Creek | CA |
| 86 | 1:37.60 | Hannah Raspopovich (11), Rachel Bukowski (9 ), Caitlyn Lowry (12), Hannah Schuster (11) | Crown Point High School | Crown Point | IN |
| 87 | 1:37.61 | Rebecca Sears (11), Eleanor Gabriel (10), Elly Faulkner (12), Miranda Mercke (11) | Sacred Heart Academy | Louisville | KY |
| 88 | 1:37.71 | Dina Rommel (9 ), Rebecca Anthone (9 ), Sydney Modeas (9 ), Claire Willis (10) | Clarence High School | Clarence | NY |
| 89 | 1:37.75 | Mekenna Scheitlin (9 ), Ashleigh Kleinjan (10), Kate Valentine (12), Erika Elliott (11) | Barrington High School | Barrington | IL |
| 90 | 1:37.77 | Morgan Contino (10), Jaymie Polet (12), Rachel Polanco (10), Valerie Borger (11) | Turpin High School | Cincinnati | OH |
| 91 | 1:37.80 | Christiana Lasich (12), Cassandra Buzby (12), Emily Jiang (9 ), Brianna Weinstein (11) | Irvine High School | Irvine | CA |
| 92 | 1:37.81 | Danielle Siverling (12), Caitlin Manthe (9 ), Lauren Tecce (12), Megan Siverling (12) | Downingtown East High School | Exton | PA |
| 93 | 1:37.83 | Kali Conlon (10), Jolie Wong (12), Melissa Lager (11), Meghan van Raalte (11) | California High School | San Ramon | CA |
| 93 | 1:37.83 | Olivia Garrett (10), Kerry Grosshans (10), Jennifer Coady (11), Genevieve Heidkamp (11) | Hinsdale Central High School | Hinsdale | IL |
| 95 | 1:37.85 | Dani Orlandi (10), Danielle Ferraro (12), Taylor Nanfria (12), Jenny Barcellona (10) | San Ramon Valley High School | Danville | CA |
| 96 | 1:37.86 | Rachel Bootsma (11), Kira Zubar (9 ), Alyssa Swanson (9 ), Ellen Suek (10) | Eden Prairie High School | Eden Prairie | MN |
| 96 | 1:37.86 | Kim Hill (9 ), Samantha Hill (11), Jesse Evans (12), Erin Calderoni (12) | Greenwich High School | Greenwich | CT |
| 96 | 1:37.86 | Britta Sortland (12), Mara Miller (11), Cassie Cedarholm (12), Mikayla Mayer (10) | Woodbury High School | Woodbury | MN |
| 99 | 1:37.87 | Lexi Nash (12), Becca Bogle (10), Jen Law (11), Amy Novak (10) | Naperville North High School | Naperville | IL |
| 100 | 1:37.88 | Jenna Egan (9 ), Ali Rusch (11), Cara Egan (11), Maddie Rusch (10) | New Canaan High School | New Canaan | CT |