| Place | Time | Swimmers (Yrs) | School | City | State |
| 1 | 1:40.73 | Madison White (10), Allison Gargalikis (12), Christina Ray (11), Chelsea Chenault (10) | Carondelet High School | Concord | CA |
| 2 | 1:41.42 | Lacey Locke (10), Samantha Shaheen (12), Ariana Bullard (10), Carly Marshall (12) | Carmel High School | Carmel | IN |
| 3 | 1:41.87 | Arden Pitman (12), Ashley Vance (12), Ashley Yearwood (11), Kristen Vredeveld (10) | The Baylor School | Chattanooga | TN |
| 4 | 1:43.00 | Caitlyn Forman (11), Molly Hinken (10), Ellen Williamson (12), Mackenzie Margroum (10) | Notre Dame Academy | Park Hills | KY |
| 5 | 1:43.02 | Sarah Lohman (11), Tori Bagan (12), Hanna House (9 ), Whitney Carroll (9 ) | Homestead High School | Fort Wayne | IN |
| 6 | 1:43.56 | Jillian Vitarius (11), Madeline Hazle (10), Leah Pfitzer (10), Jamie Friderichs (11) | Kingwood High School | Kingwood | TX |
| 7 | 1:43.74 | Taylor Sneed (12), Madeline Maher (11), Bridget Casey (12), Sarah Aemisegger (12) | Germantown Academy | Fort Washington | PA |
| 8 | 1:43.82 | Emily Baturka (11), Rachel Repke (10), Emily Rhodes (9 ), Tara O'Prey (10) | Episcopal Academy | Newtown Square | PA |
| 8 | 1:43.82 | Devin Madsen (11), Taylor Carlson (12), Jaclyn Amog (12), Kelsey Leonard (10) | Las Lomas High School | Walnut Creek | CA |
| 10 | 1:43.86 | Kellie Barbiea (12), Jennifer Garzinski (12), Dani Barbiea (12), Ashley Whiteley (10) | Springboro High School | Springboro | OH |
| 11 | 1:44.15 | Gabby Sims (9 ), Allison Soper (12), Haley Sims (12), Maddy Sims (9 ) | Downers Grove North High School | Downers Grove | IL |
| 12 | 1:44.24 | Lauren Driscoll (11), Lindsey McKnight (11), Chantal Bouthillier (11), Jessica Randolph (10) | American Heritage School | Plantation | FL |
| 13 | 1:44.25 | Kendese Nangle (11), Meghan Mcguirk (11), Carolyn Fittin (12), Molly Mucciarone (11) | The Peddie School | Hightstown | NJ |
| 14 | 1:44.35 | Chandler Soapes (10), Ashley Neidigh (10), Megan Fonteno (12), Stacey Stanfield (11) | The Bolles School | Jacksonville | FL |
| 15 | 1:44.45 | Taylor Straub (12), Kylie Reiter (11), Erin Timochenko (11), Alexandra Rockett (9 ) | Wilson High School | West Lawn | PA |
| 16 | 1:44.52 | Jenny Barcellona (10), Heidi Poppe (9 ), Marissa Neel (11), Allison Brown (11) | San Ramon Valley High School | Danville | CA |
| 17 | 1:44.55 | Missy Franklin (10), Meagan Smith (12), Alex Todd (12), Delaney Lanker (10) | Regis Jesuit High School | Aurora | CO |
| 18 | 1:44.58 | Yurie Nakano (12), Carolyn McCarty (11), Samantha Surfus (10), Madelyn Veith (9 ) | Hershey High School | Hershey | PA |
| 19 | 1:44.60 | Cheyenne Low (10), Margaret Burton (10), Olivia Evans (12), Margaret Hofstadter (11) | University High School | Irvine | CA |
| 20 | 1:44.67 | Ashley LeClair (9 ), Miranda Mercke (11), Summer Stephens (12), Eleanor Gabriel (10) | Sacred Heart Academy | Louisville | KY |
| 21 | 1:44.70 | Kate Jones (11), Mary Warren (9 ), Miranda Helbig (10), Holly Johnson (12) | Arrowhead Union High School | Hartland | WI |
| 22 | 1:44.73 | Maria Bargardi (12), Halie Zulch (11), Taylor Steffl (12), Nikki Barczak (12) | Mercy High School | Farmington Hills | MI |
| 23 | 1:44.77 | Jessica Sutherland (10), Riley Hayward (10), Samantha James (11), Megan Marchuk (12) | New Trier High School | Winnetka | IL |
| 24 | 1:44.93 | Olivia Smoliga (10), Hallie Hengels (11), Kimberly Durkin (10), Niki Wells (10) | Glenbrook South High School | Glenview | IL |
| 25 | 1:44.98 | Madeleine Klichowski (12), Michelle Garland (11), Mallory Remick (11), Evan Swenson (10) | Loyola Academy | Wilmette | IL |
| 26 | 1:45.01 | Samantha Bosma (12), Shannon O'Malley (12), Jordan Drake (10), Kim Carducci (12) | Lassiter High School | Marietta | GA |
| 27 | 1:45.04 | Emma Wyke (10), Myra Retrum (11), Kristin VanDeventer (11), Sollman Rachael (10) | Columbus North High School | Columbus | IN |
| 28 | 1:45.22 | Molly Hazelbaker (11), Gabbie Pettinichi (12), Morgan Contino (10), Valerie Borger (11) | Turpin High School | Cincinnati | OH |
| 29 | 1:45.50 | Sydney Johansen (12), Carissa Metcalf (12), Cora Stebbins (11), Alex Rieger (9 ) | St. Francis High School | Sacramento | CA |
| 30 | 1:45.53 | Shaya Schaedler (11), Ashley Ochsner (12), Kelsey Ochsner (9 ), Jade Ely (11) | Campbell County High School | Gillette | WY |
| 31 | 1:45.67 | Mickey Melosi (10), Katie Olsen (12), Lauren Torres (12), Mary Geavaras (10) | Desert Vista High School | Phoenix | AZ |
| 32 | 1:45.69 | Lyndsie Gibson (11), Elise Weisert (11), Taylor Derevyanik (11), Emily Zapinski (10) | Southlake Carroll High School | Southlake | TX |
| 33 | 1:45.72 | Marissa Cominelli (10), Elizabeth Aronoff (11), Carrie Bencic (10), Caroline Vexler (10) | Hawken School | Gates Mills | OH |
| 34 | 1:45.73 | Meghan Mendez (11), Breanna Molinaro (10), Mackenzie Meixner (11), Alyssa Ruffing (9 ) | Franklin Regional High School | Murrysville | PA |
| 35 | 1:45.80 | Haley Smith (12), Eva Chen (12), Charlotte Ruby (11), Mackenzie Chock (10) | Archbishop Mitty High School | San Jose | CA |
| 35 | 1:45.80 | Shirley Wang (12), Natalie Sanchez (12), Dayna Gettel (11), Melissa Leonhardt (11) | Plano Senior High School | Plano | TX |
| 37 | 1:45.83 | Margaret Cooke (10), Victoria Eberwein (9 ), Alexandra Ford (9 ), Alexandria Sorensen (11) | The Woodlands High School | The Woodlands | TX |
| 38 | 1:45.91 | Victoria Tschoke (10), Hayley Tomlinson (11), Jessica Godlewski (12), Jordyn Barham (9 ) | Apex High School | Apex | NC |
| 39 | 1:45.94 | Corinne Talhouk (12), Gaby Borza (10), Erin Bishop (11), Allie Pennetti (11) | North Allegheny Senior High School | Wexford | PA |
| 40 | 1:46.10 | Morgan Duncan (11), Elizabeth Garreau (11), Kelsey Gouge (11), Meghan Faulkner (12) | Brookwood High School | Snellville | GA |
| 41 | 1:46.12 | Courtney Bartholomew (11), Emma Shaw (12), Abi Johns (9 ), Nicole Cordero (11) | Holland High School | Holland | MI |
| 42 | 1:46.24 | Alexandra DeLoof (11), Megan Brooks (11), Anne Crowley (9 ), Gabrielle DeLoof (9 ) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 43 | 1:46.27 | Hayley Johnson (12), Claire Schelske (12), Jaynie Pulte (11), Clare Nienstedt (9 ) | Marian High School | Bloomfield Hills | MI |
| 44 | 1:46.31 | Allison Deperalta (11), Kelsey Kafka (10), Allison Paris (12), Anique Hermann (12) | Torrey Pines High School | San Diego | CA |
| 45 | 1:46.32 | Kayla Grim (12), Megan Polaha (10), Emily Poland (12), Emily Stang (11) | Parkland High School | Allentown | PA |
| 46 | 1:46.38 | Gia Dalesandro (10), Megan Childs (10), Brooke Bedford (10), Margaret Maxstadt (11) | Neuqua Valley High School | Naperville | IL |
| 47 | 1:46.40 | Konnor Rist (11), Ellie Grimes (10), Emily Holt (11), Jordan Caravona (10) | DuPont Manual High School | Louisville | KY |
| 48 | 1:46.44 | Allie Roberts (12), Danielle Thomas (9 ), Taylor O'Brien (11), Taylor Iobst (10) | Emmaus High School | Emmaus | PA |
| 49 | 1:46.46 | Anna Farnsworth (10), Emily Pate (11), Emily Zheng (12), Susanne Gingher (12) | State College Area High School | State College | PA |
| 50 | 1:46.53 | Mary Schneider (9 ), Haley Wickham (10), Veronica Gibson (11), Maureen Barron (11) | Fenwick High School | Oak Park | IL |
| 51 | 1:46.57 | Jessi Carpenter (11), Andrea Cottrell (10), Brianna Archambeau (12), Taylor Shotwell (12) | Bishop Hartley High School | Columbus | OH |
| 51 | 1:46.57 | Maddie Miller (11), Olivia Kabacinski (11), Meaghan McLaughlin (12), Lauren Foor (12) | Chesterton High School | Chesterton | IN |
| 51 | 1:46.57 | Tara Halsted (10), Kathleen Benjamin (9 ), Beverly Nguyen (11), Marissa La Freniere (10) | Davis Senior High School | Davis | CA |
| 54 | 1:46.60 | Sabrina Lee (12), Laura Cui (11), Margaret Wenzlau (11), Sarah Liang (12) | Palo Alto High School | Palo Alto | CA |
| 55 | 1:46.65 | Francesca Devine (12), Molly Coonce (11), Sarah Sykstus (11), Katherine Hare (11) | Rosary High School | Aurora | IL |
| 56 | 1:46.67 | Maddie Bro (11), Sydney Sorenson (9 ), Lauren Rosser (11), Taylor Flummerfelt (10) | Ames High School | Ames | IA |
| 56 | 1:46.67 | Danielle Yoon (10), Briana Burns (9 ), Hannah Cooper (12), Rachel Moody (12) | Memorial High School | Houston | TX |
| 58 | 1:46.68 | Colleen VandePoel (10), Shelby Lacy (12), Anna Battistello (10), Caroline Fender (10) | Holland West Ottawa High School | Holland | MI |
| 59 | 1:46.83 | Betsy Trace (9 ), Elizabeth Long (11), Claire VanFossen (11), Jessica Miller (11) | Upper Arlington High School | Upper Arlington | OH |
| 60 | 1:46.87 | Ellie Morrissey (11), Kylie Boyce (10), Abigail Oshiro (11), Alys Williams (11) | Edison High School | Huntington Beach | CA |
| 61 | 1:46.91 | Catherine Breed (12), Tory Houston (12), Natalie Wong (12), Kirsten Brand (10) | Amador Valley High School | Pleasanton | CA |
| 62 | 1:46.94 | Kelsey Csumitta (10), Alana Arnold (12), Logan Arnold (11), Rachel Ciferno (11) | Mayfield High School | Mayfield Village | OH |
| 63 | 1:46.99 | Katie Miller (10), Emma Durante (12), Alyssa Athmer (11), Jillian Stoneburg (11) | Akron Firestone High School | Akron | OH |
| 64 | 1:47.02 | Leah Goldman (9 ), Madison Gebhard (11), Naomi Thomas (11), Kristen Brennand (11) | Burlingame High School | Burlingame | CA |
| 65 | 1:47.05 | Victoria Mitchell (12), Basira Knight (9 ), Megan Owens (9 ), Mary Jessie Densmore (11) | Cary High School | Cary | NC |
| 65 | 1:47.05 | Sidney Cooke (10), Linda Lu (10), Lauren Damian (12), Mackenzie Milham (10) | Monte Vista High School | Danville | CA |
| 67 | 1:47.11 | Summer Schmitt (10), Kathryn Coughlin (10), Lexy McCauley (10), Maggie Fletcher (10) | Christian Brothers Academy | Syracuse | NY |
| 67 | 1:47.11 | Adelyn Graf (11), Erin Dunstan (10), Emma Tobey (11), Maddie Rusch (10) | New Canaan High School | New Canaan | CT |
| 67 | 1:47.11 | Katherine Kerman (11), Lilly Nunnally (10), Nicole Stafford (10), Rachel Astles (11) | Westminster Schools | Atlanta | GA |
| 70 | 1:47.19 | Maddy Frost (10), Cristina Czyrka (10), Emily Lau (10), Nikki Flynn (11) | Saline High School | Saline | MI |
| 71 | 1:47.26 | Joyce Zhou (10), Jacqui Levere (12), Stefanie Scherp (10), Maddy Schaefer (12) | Saint Francis High School | Mountain View | CA |
| 72 | 1:47.28 | Ashley Mitson (12), Annie Lazor (11), Hanna Pfershy (9 ), Elise Dalley (12) | Birmingham Groves High School | Beverly Hills | MI |
| 73 | 1:47.29 | Alex Wheatley (11), Emeline Leyens (10), Erin Regan (12), Lindsey Schmidt (11) | Upper Dublin High School | Fort Washington | PA |
| 74 | 1:47.33 | Amy Katz (12), Rachel Hayden (9 ), Alexandra Hofmann (12), Kellie La Mothe (11) | North Central High School | Indianapolis | IN |
| 75 | 1:47.38 | Madeline Bradford (11), Annie Spalding (9 ), Bethany Galat (9 ), Sarah Coyne (11) | Penn High School | Mishawaka | IN |
| 76 | 1:47.50 | Maureen Sullivan (9 ), Morgan Emter (11), Katie Heywood (12), Betsy Stemple (12) | Mason High School | Mason | OH |
| 77 | 1:47.52 | Brittany Dudzinski (10), Allison Kearney (12), Natalie Johnsen (11), Betsy Erlanger (10) | Upper St. Clair High School | Pittsburgh | PA |
| 78 | 1:47.53 | Claire Rutscher (10), Mady Gockowski (11), Kristin Erf (9 ), Torie Buberl (12) | Stillwater Area High School | Stillwater | MN |
| 78 | 1:47.53 | Claudia Doyle (12), Maren Reeder (10), Julia Valentine (9 ), Katie Allison (11) | Thomas Worthington High School | Worthington | OH |
| 80 | 1:47.58 | Jennifer Marrkand (9 ), Kelly Huber (10), Courteney Martin (9 ), Victoria Weinstock (9 ) | Westford Academy | Westford | MA |
| 81 | 1:47.61 | Brittany Gilbert (11), Emily Wimmer (10), Ashley Hwang (9 ), Elaine Pitsas (12) | Munster High School | Munster | IN |
| 82 | 1:47.69 | Erin Oeltjen (10), Karen Illg (12), Brooke Stilmock (10), Faith Daley (12) | Millard West High School | Omaha | NE |
| 83 | 1:47.70 | Sierra Robbins (12), Shelly Caron (12), Samantha Swinton (11), Erin Schoenfeld (9 ) | Miramonte High School | Orinda | CA |
| 84 | 1:47.71 | Kaylee Kucera (11), Jordan Ford (10), Audrey Kumm (12), Aspen Billiet (11) | Hutchinson High School | Hutchinson | MN |
| 85 | 1:47.75 | Janet Hu (9 ), Melissa Shebat (10), Kaitlin Pawlowicz (12), Stephanie Hallock (11) | Oakton High School | Vienna | VA |
| 86 | 1:47.76 | Margaret D'Innocenzo (10), Ashlee Korsberg (10), Rachel Moore (11), Emma Cammann (10) | Andover High School | Andover | MA |
| 87 | 1:47.80 | Madeleine Eden (10), Heather Laedtke (10), Nikki Larson (11), Adrienne Hebb (12) | Edina High School | Edina | MN |
| 88 | 1:47.86 | Lauryn Brown (12), Catherine MacKeen (10), Megan Griffin (11), Christina Hurley (12) | Lincoln Sudbury Regional High School | Sudbury | MA |
| 88 | 1:47.86 | Christina Leander (10), Jennifer Mill (10), Darrah Jampo (10), Jacqueline Caruso (11) | West Chester East High School | West Chester | PA |
| 90 | 1:47.95 | Carolyn Stewart (12), Brittany Morguelan (12), Michelle Gasior (12), Rebecca Mueller (9 ) | Assumption High School | Louisville | KY |
| 91 | 1:48.03 | Annie Tamblyn (11), Anna DeMonte (11), Elizabeth Gelb (12), Jessa Gargan (10) | Ann Arbor Huron High School | Ann Arbor | MI |
| 92 | 1:48.05 | Jessica Cronin (11), Megan Kaveney (12), Anna Fetter (12), Caroline Baldwin (9 ) | Westfield High School | Westfield | NJ |
| 93 | 1:48.07 | Ryann Lawley (10), Amber Jenkins (12), Melanie McDonough (12), Allie Walsh (10) | Frisco High School | Frisco | TX |
| 94 | 1:48.08 | Caroline Patterson (12), Kourtney Fosse (11), Lacey Smith (10), Zoe Verhoeven (11) | Fairview High School | Boulder | CO |
| 95 | 1:48.10 | Kathryn Comer (10), Stephanie Ferrell (12), Carly Whitmer (10), Cassandra Sosnovich (11) | Mount Saint Mary Academy | Watchung | NJ |
| 96 | 1:48.11 | Kasey Fontaine (11), Kenna Nagy (11), Kendyl Stewart (11), Kelly Lawson (10) | La Costa Canyon High School | Carlsbad | CA |
| 97 | 1:48.14 | Mary Sinatra (11), Lia Lombardi (10), Alyssa Yambor Maul (10), Delaney Barnard (9 ) | Lawton Chiles High School | Talahassee | FL |
| 97 | 1:48.14 | Jax Shoults (9 ), Megan Gehrich (11), Raquel Remick (9 ), Megan Roby (9 ) | Santa Margarita Catholic High School | Rancho Santa Margarita | CA |
| 99 | 1:48.20 | Abigail Meyer (9 ), Koryn Ternes (11), Erin Flanigan (12), Nadia El-Hamdi (12) | Bowie High School | Austin | TX |
| 99 | 1:48.20 | Ashley Wayman (10), Bridget Laguna (12), Maya Owens (11), Danielle Spradlin (12) | Converse Judson High School | Converse | TX |