| Place | Time | Name (Yr) | School | City | State |
| 1 | :51.99 | Courtney Bartholomew (12) | Holland High School | Holland | MI |
| 2 | :52.64 | Missy Franklin (11) | Regis Jesuit High School | Aurora | CO |
| 3 | :53.45 | Janet Hu (10) | Oakton High School | Vienna | VA |
| 4 | :53.60 | Lindsey Engel (10) | Crean Lutheran High School | Irvine | CA |
| 5 | :53.89 | Caitlyn Forman (12) | Notre Dame Academy | Park Hills | KY |
| 6 | :53.95 | Ashlee Linn (12) | Riverview High School | Sarasota | FL |
| 7 | :53.97 | Tasija Karosas (11) | Saint Andrew's School | Boca Raton | FL |
| 8 | :54.04 | Kimberlee John-Williams (11) | The Baylor School | Chattanooga | TN |
| 9 | :54.12 | Anika Apostalon (11) | Albuquerque Academy | Albuquerque | NM |
| 10 | :54.14 | Christina Leander (11) | West Chester East High School | West Chester | PA |
| 11 | :54.17 | Kaitlyn Jones (11) | The Tatnall School | Wilmington | DE |
| 12 | :54.22 | Madison Bridges (12) | Albuquerque High School | Albuquerque | NM |
| 13 | :54.23 | Emily Slabe (10) | Ursuline Academy | Cincinnati | OH |
| 14 | :54.30 | Madison White (11) | Carondelet High School | Concord | CA |
| 15 | :54.33 | Catherine Galletti (12) | Trinity Preparatory School | Winter Park | FL |
| 16 | :54.43 | Alexis Bullard (12) | Carmel High School | Carmel | IN |
| 17 | :54.54 | Maggie Gruber (11) | Mars Area High School | Mars | PA |
| 18 | :54.56 | Anna Demonte (12) | Ann Arbor Huron High School | Ann Arbor | MI |
| 19 | :54.58 | Rachel Moore (12) | Andover High School | Andover | MA |
| 20 | :54.60 | Katie Kinnear (12) | Skyline High School | Sammamish | WA |
| 21 | :54.67 | Marie Georger (12) | Mercyhurst Preparatory School | Erie | PA |
| 22 | :54.68 | Alexandra DeLoof (12) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 23 | :54.70 | Brittany Robinson (12) | Wawesee High School | Syracuse | IN |
| 24 | :54.73 | Henriette Stenkvist (12) | Panther Creek High School | Cary | NC |
| 25 | :54.75 | Marissa Cominelli (11) | Hawken School | Gates Mills | OH |
| 26 | :54.78 | Samantha Wicks (12) | East High School | Anchorage | AK |
| 27 | :54.79 | Sidney Cooke (11) | Monte Vista High School | Danville | CA |
| 28 | :54.85 | Kristina Li (10) | Thomas S. Wootton High School | Rockville | MD |
| 29 | :54.89 | Sarah Hitchens (11) | Villa Maria Academy | Malvern | PA |
| 30 | :54.90 | Shelly Patton (12) | Heritage High School | Littleton | CO |
| 31 | :54.91 | Morgan Pfaff (12) | Red Lion Area Senior High School | Red Lion | PA |
| 32 | :54.92 | Hanna House (10) | Carmel High School | Carmel | IN |
| 33 | :55.11 | Lauren Church (12) | Greenwich High School | Greenwich | CT |
| 34 | :55.14 | Emma Paulson (11) | Wayzata High School | Plymouth | MN |
| 35 | :55.18 | Kathleen Baker (9 ) | Forsyth Country Day School | Lewisville | NC |
| 36 | :55.19 | Holly Harper (12) | Albemarle High School | Charlottesville | VA |
| 37 | :55.21 | Gabby Sims (10) | Downers Grove North High School | Downers Grove | IL |
| 38 | :55.23 | Alexandra Tracy (11) | Indian Hill High School | Cincinnati | OH |
| 39 | :55.25 | Mackenzie Buss (11) | Shawano Community High School | Shawano | WI |
| 40 | :55.27 | Lacey Locke (11) | Carmel High School | Carmel | IN |
| 41 | :55.31 | Jenna Bickel (12) | Guilderland-Voorheesville High School | Guilderland | NY |
| 42 | :55.32 | Marissa Neel (12) | San Ramon Valley High School | Danville | CA |
| 43 | :55.34 | Claire Brandt (12) | Greenhill School | Addison | TX |
| 44 | :55.35 | Katie Miller (11) | Akron Firestone High School | Akron | OH |
| 45 | :55.36 | Emily Baturka (12) | Episcopal Academy | Newtown Square | PA |
| 46 | :55.38 | Heather Merritt (10) | William A. Hough High School | Cornelius | NC |
| 47 | :55.39 | Rachael Dudley (12) | Mill Creek High School | Hoschton | GA |
| 48 | :55.42 | Margaret Cooke (11) | The Woodlands High School | The Woodlands | TX |
| 49 | :55.47 | Ella Eastin (9 ) | Crean Lutheran High School | Irvine | CA |
| 50 | :55.52 | Rebecca Baxley (11) | Analy High School | Sebastopol | CA |
| 51 | :55.53 | Bonnie Brandon (12) | Cherry Creek High School | Greenwood Village | CO |
| 52 | :55.55 | Charlotte Meyer (11) | Walt Whitman High School | Bethesda | MD |
| 53 | :55.59 | Camey Rabold (12) | Bishop Watterson High School | Columbus | OH |
| 54 | :55.62 | Marie Chamberlain (10) | Nauset Regional High School | North Eastham | MA |
| 54 | :55.62 | Caroline Korst (11) | Lewisville Hebron High School | Carrollton | TX |
| 54 | :55.62 | Jessica Shepard (12) | Dripping Springs High School | Dripping Springs | TX |
| 57 | :55.63 | Tara Halsted (11) | Davis Senior High School | Davis | CA |
| 58 | :55.66 | Lauren Comer (12) | Upper Arlington High School | Upper Arlington | OH |
| 59 | :55.67 | Natalie Amberg (10) | Carondelet High School | Concord | CA |
| 60 | :55.69 | Michele Rielly (12) | Bishop Watterson High School | Columbus | OH |
| 61 | :55.74 | Kathryn Eckhart (12) | Cy Creek High School | Houston | TX |
| 62 | :55.76 | Devin Madsen (12) | Las Lomas High School | Walnut Creek | CA |
| 62 | :55.76 | Katie Siegel (12) | Harker | San Jose | CA |
| 62 | :55.76 | Olivia Smoliga (11) | Glenbrook South High School | Glenview | IL |
| 65 | :55.77 | Mallory Buha (9 ) | Novi High School | Novi | MI |
| 66 | :55.79 | Morgan Fairclough (11) | Monroe Woodbury High School | Central Valley | NY |
| 66 | :55.79 | Hellen Moffitt (11) | West Potomac High School | Alexandria | VA |
| 68 | :55.87 | Cheyenne Low (11) | University High School | Irvine | CA |
| 69 | :55.88 | Ellen Bloom (11) | Sartell High School | Sartell | MN |
| 70 | :55.89 | Jax Shoults (10) | Santa Margarita Catholic High School | Rancho Santa Margarita | CA |
| 71 | :55.91 | Casey Fazio (12) | Immaculate Heart Academy | Township Of Washington | NJ |
| 72 | :55.97 | Caroline Baldwin (10) | Westfield High School | Westfield | NJ |
| 73 | :55.98 | Addison Ferguson (12) | Cloverleaf High School | Lodi | OH |
| 74 | :55.99 | Brittany Gilbert (12) | Munster High School | Munster | IN |
| 74 | :55.99 | Jennifer Marrkand (10) | Westford Academy | Westford | MA |
| 74 | :55.99 | Emma Szczupakiewicz (12) | Muskego High School | Muskego | WI |
| 77 | :56.00 | Gabriella Schaffer (12) | Muhlenberg High School | Laureldale | PA |
| 78 | :56.01 | Lyndsie Gibson (12) | Southlake Carroll High School | Southlake | TX |
| 78 | :56.01 | Marina Spadoni (12) | Mission Viejo High School | Mission Viejo | CA |
| 80 | :56.02 | Elizabeth Auckley (10) | Bay High School | Bay Village | OH |
| 81 | :56.05 | Chelsea Tatlow (10) | Rock Bridge High School | Columbia | MO |
| 81 | :56.05 | Aly Tetzloff (9 ) | Crown Point High School | Crown Point | IN |
| 83 | :56.08 | Sophie Webber (12) | Granite Bay High School | Granite Bay | CA |
| 84 | :56.10 | Hannah Peiffer (11) | The Baylor School | Chattanooga | TN |
| 85 | :56.11 | Alyssa Yambor-Maul (11) | Lawton Chiles High School | Tallahassee | FL |
| 86 | :56.14 | Anna Grainger (12) | Ensworth High School | Nashville | TN |
| 86 | :56.14 | Elise Haan (9 ) | Gulf Coast High School | Naples | FL |
| 88 | :56.16 | Riley Marks (12) | Johnson High School | San Antonio | TX |
| 88 | :56.16 | Konnor Rist (12) | duPont Manual High School | Louisville | KY |
| 90 | :56.20 | Eliza Bragg (11) | Deerfield Academy | Deerfield | MA |
| 90 | :56.20 | Inez Charles (10) | Bloomfield Hills Lahser High School | Bloomfield Hills | MI |
| 92 | :56.25 | Karen Turner (12) | Lakeridge High School | Lake Oswego | OR |
| 93 | :56.27 | Shelby Carroll (12) | Lake Central High School | St. John | IN |
| 94 | :56.31 | Kate Santilena (11) | Chaparral High School | Temecula | CA |
| 95 | :56.37 | Brittany Dudzinski (11) | Upper St. Clair High School | Upper St. Clair | PA |
| 95 | :56.37 | Alisabeth Marsteller (10) | Ursuline Academy | Cincinnati | OH |
| 97 | :56.39 | Larah Beaver (12) | Yorktown High School | Yorktown | IN |
| 97 | :56.39 | Megan Young (11) | Etowah High School | Woodstock | GA |
| 99 | :56.40 | Madison Myers (9 ) | Copley High School | Copley | OH |
| 100 | :56.41 | Maddy Frost (11) | Saline High School | Saline | MI |