| Place | Time | Name (Yr) | School | City | State |
| 1 | :52.36 | Jasmine Tosky (12) | Palo Alto High School | Palo Alto | CA |
| 2 | :53.07 | Celina Li (11) | Foothill High School | Pleasanton | CA |
| 3 | :53.20 | Janet Hu (10) | Oakton High School | Vienna | VA |
| 4 | :53.41 | Brittany Robinson (12) | Wawesee High School | Syracuse | IN |
| 5 | :53.46 | Katie Kinnear (12) | Skyline High School | Sammamish | WA |
| 6 | :53.50 | Maggie Gruber (11) | Mars Area High School | Mars | PA |
| 7 | :53.51 | Mary Griffith (12) | Tokay High School | Lodi | CA |
| 8 | :53.57 | Mimi Schneider (10) | Fenwick High School | Oak Park | IL |
| 9 | :53.70 | Katie McLaughlin (9 ) | JSerra Catholic High School | San Juan Capistrano | CA |
| 10 | :53.72 | Kelsi Worrell (12) | Rancocas Valley Regional High School | Mount Holly | NJ |
| 11 | :53.75 | Marissa Neel (12) | San Ramon Valley High School | Danville | CA |
| 12 | :53.93 | Ella Eastin (9 ) | Crean Lutheran High School | Irvine | CA |
| 13 | :53.97 | Danielle Nack (10) | Mankato West High School | Mankato | MN |
| 14 | :54.20 | Dana Grindall (11) | Amity High School | Woodbridge | CT |
| 15 | :54.27 | Madison Martin (11) | Pickerington High School Central | Pickerington | OH |
| 15 | :54.27 | Rachel Moore (12) | Andover High School | Andover | MA |
| 17 | :54.38 | Brynne Wong (11) | Corona Del Mar High School | Newport Beach | CA |
| 18 | :54.39 | Taylor Garcia (9 ) | Holland High School | Holland | MI |
| 19 | :54.46 | Rachael Dudley (12) | Mill Creek High School | Hoschton | GA |
| 20 | :54.49 | Christina Ray (12) | Carondelet High School | Concord | CA |
| 21 | :54.51 | Heather Lundstrom (11) | Parkway North High School | St. Louis | MO |
| 22 | :54.58 | Hilvy Cheung (11) | American High School | Fremont | CA |
| 23 | :54.62 | Bailey Nero (9 ) | Fossil Ridge High School | Fort Collins | CO |
| 24 | :54.63 | Breanna Sapienza (10) | Cascade Christian High School | Medford | OR |
| 25 | :54.66 | Olivia Barker (11) | Bishop Gorman High School | Las Vegas | NV |
| 25 | :54.66 | Connie Hsu (12) | Glenbard West Co-Op | Glen Ellyn | IL |
| 25 | :54.66 | Sarah Koucheki (11) | Hawken School | Gates Mills | OH |
| 28 | :54.68 | Hannah Lincoln (12) | Leesville Road High School | Raleigh | NC |
| 29 | :54.72 | Sonia Wang (9 ) | Upland High School | Upland | CA |
| 30 | :54.75 | Justine Panian (12) | Mission Hills High School | San Marcos | CA |
| 31 | :54.76 | Beverly Nguyen (12) | Davis Senior High School | Davis | CA |
| 32 | :54.77 | Angela Algee (11) | Dr. Phillips High School | Orlando | FL |
| 33 | :54.82 | Madison Wright (9 ) | Waterford United High Schools | Waterford | MI |
| 34 | :54.89 | Gabby Sims (10) | Downers Grove North High School | Downers Grove | IL |
| 35 | :54.90 | Frida Berggren (11) | Lewis-Palmer High School | Monument | CO |
| 36 | :54.92 | Kayla Skenandore (12) | Waukesha South High School | Waukesha | WI |
| 37 | :54.93 | Gia Dalesandro (11) | Neuqua Valley High School | Naperville | IL |
| 38 | :54.94 | Charlotte Ruby (12) | Archbishop Mitty High School | San Jose | CA |
| 39 | :54.96 | Kimberlee John-Williams (11) | The Baylor School | Chattanooga | TN |
| 40 | :55.04 | Kayla Moran (11) | Lyman High School | Longwood | FL |
| 41 | :55.08 | Hanna House (10) | Carmel High School | Carmel | IN |
| 41 | :55.08 | Hellen Moffitt (11) | West Potomac High School | Alexandria | VA |
| 41 | :55.08 | Grace Wold (11) | Mercer Island High School | Mercer Island | WA |
| 44 | :55.09 | Natalya Ares (12) | Winston Churchill High School | Potomac | MD |
| 44 | :55.09 | Nikki Larson (12) | Edina High School | Edina | MN |
| 46 | :55.12 | Claire Van Fossen (12) | Upper Arlington High School | Upper Arlington | OH |
| 47 | :55.14 | Bailie Monahan (12) | Colonial Forge High School | Stafford | VA |
| 48 | :55.24 | Joyce Kwok (12) | Francis Lewis High School | Long Beach | NY |
| 49 | :55.26 | Katie Miller (11) | Akron Firestone High School | Akron | OH |
| 49 | :55.26 | Aly Tetzloff (9 ) | Crown Point High School | Crown Point | IN |
| 51 | :55.29 | Caitlin Casazza (10) | Bishop McGuinness Catholic High School | Kernersville | NC |
| 51 | :55.29 | Jennifer Marrkand (10) | Westford Academy | Westford | MA |
| 51 | :55.29 | Megan Moroney (9 ) | Saint Andrew's School | Boca Raton | FL |
| 54 | :55.31 | Carrie Johnson (9 ) | Mount Juliet High School | Mount Juliet | TN |
| 55 | :55.33 | Genna Heidkamp (12) | Hinsdale Central High School | Hinsdale | IL |
| 55 | :55.33 | Alyssa Yambor-Maul (11) | Lawton Chiles High School | Tallahassee | FL |
| 57 | :55.39 | Naomi Thomas (12) | Burlingame High School | Burlingame | CA |
| 57 | :55.39 | Taylor Weiss (10) | Etowah High School | Woodstock | GA |
| 59 | :55.40 | Katherine Diller (11) | Manheim Township High School | Lancaster | PA |
| 60 | :55.42 | Jordan Bullock (12) | Zeeland High School | Zeeland | MI |
| 61 | :55.47 | Katie Siegel (12) | Harker | San Jose | CA |
| 62 | :55.51 | Molly Coonce (12) | Rosary High School | Aurora | IL |
| 63 | :55.52 | Anna Quinn (10) | Thousand Oaks High School | Thousand Oaks | CA |
| 64 | :55.53 | Morgan Karetnick (12) | Greenwich High School | Greenwich | CT |
| 65 | :55.55 | Claire Brandt (12) | Greenhill School | Addison | TX |
| 66 | :55.56 | Brittany Usinger (9 ) | Acalanes High School | Lafayette | CA |
| 67 | :55.58 | Jasmine Mau (10) | Punahou School | Honolulu | HI |
| 67 | :55.58 | Emily Slabe (10) | Ursuline Academy | Cincinnati | OH |
| 69 | :55.62 | Cara Norris (9 ) | Sycamore High School | Cincinnati | OH |
| 70 | :55.65 | Haley Clark (12) | Travis B. Bryan High School | Bryan | TX |
| 71 | :55.66 | Megan Polaha (11) | Parkland High School | Allentown | PA |
| 71 | :55.66 | Ritty Zhai (12) | Archbishop Mitty High School | San Jose | CA |
| 73 | :55.70 | Summer Schmitt (11) | Christian Brothers Academy | Syracuse | NY |
| 73 | :55.70 | Nicole Stafford (11) | Westminster Schools | Atlanta | GA |
| 75 | :55.72 | Colby Harvey (10) | Waynflete School | Portland | ME |
| 76 | :55.73 | Ashley Johnson (9 ) | Langham Creek High School | Houston | TX |
| 76 | :55.73 | Sarah Sykstus (12) | Rosary High School | Aurora | IL |
| 78 | :55.75 | Morgan Contino (11) | Turpin High School | Cincinnati | OH |
| 78 | :55.75 | Emily Holt (12) | duPont Manual High School | Louisville | KY |
| 78 | :55.75 | Casey Papp (12) | Bloomington High School South | Bloomington | IN |
| 81 | :55.77 | Kaitlyn Cerne (11) | Hawken School | Gates Mills | OH |
| 81 | :55.77 | Taite Kitchel (9 ) | Zionsville Community High School | Zionsville | IN |
| 83 | :55.79 | Valerie Yoshimura (9 ) | Baldwin School | Bryn Mawr | PA |
| 84 | :55.80 | Margaret Jordan (12) | Niskayuna High School | Niskayuna | NY |
| 85 | :55.81 | Brittany Gilbert (12) | Munster High School | Munster | IN |
| 85 | :55.81 | Emily Jiang (10) | Irvine High School | Irvine | CA |
| 87 | :55.83 | Hannalore Strash (11) | Alamo Heights High School | San Antonio | TX |
| 88 | :55.84 | Ariana Bullard (11) | Carmel High School | Carmel | IN |
| 89 | :55.85 | Madison Applegate (12) | Heritage High School | Brentwood | CA |
| 90 | :55.87 | Jaynie Pulte (12) | Marian High School | Bloomfield Hills | MI |
| 91 | :55.89 | Ariana Saghafi (9 ) | Albany High School | Albany | CA |
| 92 | :55.90 | Rachael Acker (12) | Gunn High School | Palo Alto | CA |
| 92 | :55.90 | Michi Aisawa (11) | Adrian C. Wilcox High School | Santa Clara | CA |
| 94 | :55.91 | Kate Santilena (11) | Chaparral High School | Temecula | CA |
| 95 | :55.93 | Grace DiMeo (10) | Booker High School | Sarasota | FL |
| 96 | :55.94 | Shelly Patton (12) | Heritage High School | Littleton | CO |
| 96 | :55.94 | Maddy Zimmerman (10) | Spring-Ford High School | Royersford | PA |
| 98 | :55.95 | Caitlyn Forman (12) | Notre Dame Academy | Park Hills | KY |
| 98 | :55.95 | Sydney Hirschi (11) | Loretto Academy | El Paso | TX |
| 98 | :55.95 | Jen Law (12) | Naperville North High School | Naperville | IL |