| Place | Time | Swimmers (Yrs) | School | City | State |
| 1 | 1:31.18 | Kristen Vredeveld (11), Bria Deveaux (12), Kimberlee John-Williams (11), Ashley Yearwood (12) | The Baylor School | Chattanooga | TN |
| 2 | 1:33.78 | Katerina Borisov (11), Allie Pennetti (12), Arielle Krigger (11), Kally Vanderbilt (12) | North Allegheny High School | Wexford | PA |
| 3 | 1:34.04 | Mimi Schneider (10), Paulina Kaminski (10), Haley Wickham (11), Maureen Barron (12) | Fenwick High School | Oak Park | IL |
| 4 | 1:34.34 | Meaghan Raab (10), Alexis Grier (12), Carly Deliberty (11), Madelyn Veith (10) | Hershey High School | Hershey | PA |
| 5 | 1:34.40 | Olivia Anderson (9 ), Emma Wittmer (9 ), Maryellen Campbell (12), Nikki Larson (12) | Edina High School | Edina | MN |
| 6 | 1:34.50 | Kaitlyn Albertoli (9 ), Karli Thuen (9 ), Rebecca Millard (11), Katie McLaughlin (9 ) | JSerra Catholic High School | San Juan Capistrano | CA |
| 7 | 1:34.63 | Alex Cleveland (10), Grace Einterz (11), Lizzy Lyon (12), Zoe Mattingly (12) | Zionsville Community High School | Zionsville | IN |
| 8 | 1:34.68 | Grace Long (9 ), Jessica Miller (12), Jennifer Smith (10), Gabrielle Veri (12) | Upper Arlington High School | Upper Arlington | OH |
| 9 | 1:34.73 | Emma Henderson (11), Margaret Hofstadter (12), Megan Rankin (12), Margaret Burton (11) | University High School | Irvine | CA |
| 10 | 1:34.75 | Meagan Dollard (10), Shannon McKernan (9 ), Natalie Dowzicky (10), Rachel Zilinskas (11) | Germantown Academy | Fort Washington | PA |
| 11 | 1:34.80 | Olivia Smoliga (11), Niki Wells (11), Anna Veselovsky (12), Katie Wells (9 ) | Glenbrook South High School | Glenview | IL |
| 12 | 1:34.87 | Rachael Acker (12), Julia Ama (12), Gabrielle Bethke (10), Casey Lincoln (12) | Gunn High School | Palo Alto | CA |
| 13 | 1:34.92 | Jessica Sutherland (11), Morgan Scott (12), Stephanie Marchuk (11), Samantha James (12) | New Trier High School | Winnetka | IL |
| 14 | 1:34.97 | Camey Rabold (12), Michele Rielly (12), Aly Francis (12), Samantha Schuttinger (11) | Bishop Watterson High School | Columbus | OH |
| 15 | 1:35.03 | Rebecca Detro (12), Lucy Kramer (12), Alexis Bullard (12), Arianna Bullard (11) | Carmel High School | Carmel | IN |
| 16 | 1:35.18 | Bethany Galat (10), Annie Spalding (10), Jennifer Priebe (11), Sarah Coyne (12) | Penn High School | Mishawaka | IN |
| 17 | 1:35.23 | Taylor Katz (11), Niam O'Grady (10), Natalie Hegwein (9 ), Ashlee Linn (12) | Riverview High School | Sarasota | FL |
| 18 | 1:35.27 | Michelle Garland (12), Mallory Remick (12), Evan Swenson (11), Claire Rushin (9 ) | Loyola Academy | Wilmette | IL |
| 19 | 1:35.32 | Kaitlyn Ruffing (11), Mackenzie Meixner (12), Julianna Carolla (12), Alyssa Ruffing (10) | Franklin Regional High School | Murrysville | PA |
| 20 | 1:35.38 | Rachel Burke (12), Erin Hart (9 ), Sarah Sykstus (12), Katherine Hare (12) | Rosary High School | Aurora | IL |
| 21 | 1:35.39 | Reia Tong (12), Sarah Kannan (10), Audrey Gould (11), Charlotte Meyer (11) | Walt Whitman High School | Bethesda | MD |
| 22 | 1:35.46 | Michaela Rookus (12), Audra Thornton (12), Makayla Myers (10), Danielle Freeman (11) | Hudsonville High School | Hudsonville | MI |
| 23 | 1:35.52 | Carolyn Kane (11), Jenine Einhorn (12), Abigail Saddler (10), Isabel Wyer (10) | Minnetonka High School | Minnetonka | MN |
| 24 | 1:35.54 | Camilla Czulada (12), Taylor Brew (12), Elizabeth Ebert (11), Alexandra Rockett (10) | Wilson High School | West Lawn | PA |
| 25 | 1:35.61 | Tasija Karosas (11), Brenna Ruth (10), Hannah Coombs (11), Megan Moroney (9 ) | Saint Andrew's School | Boca Raton | FL |
| 26 | 1:35.63 | Tara O'Prey (11), McKenzie Street (9 ), Bernadette Tankle (10), Emily Baturka (12) | Episcopal Academy | Newtown Square | PA |
| 27 | 1:35.64 | Julimar Avila (9 ), Victoria Bryson (10), Maddie Pierce (12), Bianca Tocci (12) | Weston High School | Weston | MA |
| 28 | 1:35.65 | Ritchey Howe (12), Eliza Bragg (11), Johee Hwang (11), Jenner McLeod (11) | Deerfield Academy | Deerfield | MA |
| 29 | 1:35.74 | Gabi D'Abato (10), Brooke Delventhal (9 ), Molly Washko (10), Jenna Barrus (11) | Strongsville High School | Strongsville | OH |
| 30 | 1:35.75 | Elaine Powell (11), Fiona Kane (12), Alex Bae (11), Bonnie Brandon (12) | Cherry Creek High School | Greenwood Village | CO |
| 31 | 1:35.82 | Maddie Downs (10), Erika Redding (11), Katie Christy (9 ), Rachel Astles (12) | Westminster Schools | Atlanta | GA |
| 32 | 1:35.84 | Hannah Baratz (9 ), Lauren Ulsh (12), Michelle Campeau (9 ), Kallyn Barkey (9 ) | Rochester Mayo High School | Rochester | MN |
| 32 | 1:35.84 | Kendese Nangle (12), Meghan McGuirk (12), Brynne Follman (11), Molly Mucciarone (12) | The Peddie School | Hightstown | NJ |
| 34 | 1:35.86 | Kasey Schmidt (9 ), Savannah Fernandez (12), Stacey Stanfield (12), Smacker Miles (11) | The Bolles School | Jacksonville | FL |
| 35 | 1:35.89 | Clare Nienstedt (10), Madison Taylor (11), Mollie Pulte (9 ), Jaynie Pulte (12) | Marian High School | Bloomfield Hills | MI |
| 36 | 1:35.93 | Hannah Raspopovich (12), Rachel Bukowski (10), Aly Tetzloff (9 ), Hannah Schuster (12) | Crown Point High School | Crown Point | IN |
| 37 | 1:35.94 | Alexandria Franz (12), Robyn Butler (10), Cathy Woo (11), Morgan McClure (10) | Rio Americano High School | Sacramento | CA |
| 38 | 1:36.00 | Abby Jagdfeld (10), Molly Manchon (9 ), Kayla Skenandore (12), Kaersten Meitz (10) | Waukesha South High School | Waukesha | WI |
| 39 | 1:36.07 | Kimmie Kreuzberger (12), Alexis Lee (12), Aislinn Dresel (10), Alexandra Thomason (12) | Marin Catholic High School | Kentfield | CA |
| 40 | 1:36.08 | Lindsay Schultz (11), Emily Zapinski (11), Emily Gibson (10), Taylor Derevyanik (12) | Southlake Carroll High School | Southlake | TX |
| 41 | 1:36.10 | Sarah Gibson (11), Mary Margaret Soderberg (11), Colleen Konetzke (12), Kristina Domaschofsky (9 ) | Ronald Reagan High School | San Antonio | TX |
| 42 | 1:36.12 | Emma Siewert (12), Carylyn Waite (10), Savannah Savitt (9 ), Rebecca Werdine (12) | Tampa Preparatory | Tampa | FL |
| 43 | 1:36.20 | Jenny Barcellona (11), Dani Orlandi (11), Cailey Steffens (9 ), Allison Brown (12) | San Ramon Valley High School | Danville | CA |
| 44 | 1:36.34 | Elliott Schinella (10), Madeline Loniewski (9 ), Sato Kakihara (12), Olivia Samoray (12) | Mercy High School | Farmington Hills | MI |
| 45 | 1:36.35 | Emily Albrecht (9 ), Maddy Sims (10), Gabriele Serniute (9 ), Gabby Sims (10) | Downers Grove North High School | Downers Grove | IL |
| 46 | 1:36.38 | Temarie Tomley (10), Gabrielle Young (11), Kate Pawlukiewicz (12), Alisabeth Marsteller (10) | Ursuline Academy | Cincinnati | OH |
| 47 | 1:36.39 | Abby Stone (9 ), Sydney King (10), Kelsey Haller (12), Lori Davis (12) | Granville High School | Granville | OH |
| 47 | 1:36.39 | Sarah Hitchens (11), Julie Dobson (10), Kaitlyn Alvanos (11), Julia Murphy (12) | Villa Maria Academy | Malvern | PA |
| 49 | 1:36.41 | Raquel Grays (10), Riley Marks (12), Taylor Silvestry (10), Brianna Higgins (11) | Johnson High School | San Antonio | TX |
| 50 | 1:36.43 | Hanna Pfershy (10), Beth Moran (11), Taylor Pfershy (12), Annie Lazor (12) | Birmingham Groves High School | Beverly Hills | MI |
| 51 | 1:36.45 | Sarah Koucheki (11), Mackenzie Harris (12), Caroline Vexler (11), Morgan Cohara (12) | Hawken School | Gates Mills | OH |
| 52 | 1:36.48 | Kate Boyer (9 ), Ann Claire Karalyos (11), Lauren Murski (10), Annelyse Tullier (9 ) | Highland Park High School | Dallas | TX |
| 53 | 1:36.49 | Erika McCormick (12), Clara Baggett (9 ), Mary Klueh (12), Amy Steele (12) | Mt. Vernon High School | Mt. Vernon | IN |
| 54 | 1:36.51 | Klare Northuis (12), Nicole Cordero (12), Cassie Misiewicz (10), Haley Thompson (12) | Holland High School | Holland | MI |
| 54 | 1:36.51 | Karin Roh (11), Nicole Russell (10), Laura Day (12), Frida Berggren (11) | Lewis-Palmer High School | Monument | CO |
| 54 | 1:36.51 | Riley Schroedter (11), Megan Childs (11), Tara Blanzy (12), Maggie Maxstadt (12) | Neuqua Valley High School | Naperville | IL |
| 57 | 1:36.52 | Kate Rogers (10), Emily Lau (11), Melanie Schroeder (11), Nikki Flynn (12) | Saline High School | Saline | MI |
| 58 | 1:36.56 | Louisa Hoffman (11), Kristine Rosenberger (11), Jennifer Weigand (12), Elly Vitek (11) | Lyons Township High School | Lagrange | IL |
| 59 | 1:36.58 | Maxine Clifford (12), Sara Bertram (10), Reanna Dona (12), Hellen Moffitt (11) | West Potomac High School | Alexandria | VA |
| 60 | 1:36.60 | Alex Rieger (10), Emma Barksdale (9 ), Rachel Johnson (12), Marissa La Freniere (11) | Davis Senior High School | Davis | CA |
| 61 | 1:36.62 | Tarin Anding (12), Lauren Martens (11), Molly Peichel (12), Marisa Wood (11) | Sartell High School | Sartell | MN |
| 62 | 1:36.65 | Courtney Klefeker (12), Chelsey Reber (12), Reagan Cook (10), Mariah Hamil (12) | Homestead High School | Fort Wayne | IN |
| 63 | 1:36.69 | Corinne Caldwell (12), Dierdre Gerke (10), Rachel Green (11), Stormy Malarik (10) | South Lyon High School | South Lyon | MI |
| 64 | 1:36.76 | Ashley Leclair (10), Madison Lichtefeld (10), Rebecca Sears (12), Miranda Mercke (12) | Sacred Heart Academy | Louisville | KY |
| 65 | 1:36.78 | Margaret Cooke (11), Victoria Eberwein (10), Rebekah Porter (12), Alexandria Sorensen (12) | The Woodlands High School | The Woodlands | TX |
| 66 | 1:36.80 | Amy Zhao (10), Julia Torabi (12), Molly Levene (12), Danielle Liu (10) | Phillips Academy | Andover | MA |
| 67 | 1:36.81 | Sarah Love (11), Kim Zehnder (9 ), Emily Holt (12), Jordan Caravona (11) | duPont Manual High School | Louisville | KY |
| 67 | 1:36.81 | Caitlin Cooper (10), Ellie Behr (12), Joelle Borchers (9 ), Kathleen Cook (10) | Woodward Academy | College Park | GA |
| 69 | 1:36.87 | Kaelah Chaney (11), Casey Francis (11), Hannah Veale (10), Brittany Hammond (11) | Pine Crest School | Fort Lauderdale | FL |
| 70 | 1:36.88 | Emma Blackwood (9 ), Bridget Killian (12), Sophie Pilkinton (9 ), Riley Frost (10) | Harpeth Hall | Nashville | TN |
| 71 | 1:36.91 | Mika Hlavin (12), Olivia Campbell (12), Eleanor Meeks (9 ), Rachel Stoehr (12) | Lake Forest High School | Lake Forest | IL |
| 72 | 1:36.98 | Olivia Aitken (11), Marley Maluafiti (9 ), Catie Miller (10), Amy Lubawy (10) | Bishop Gorman High School | Las Vegas | NV |
| 73 | 1:37.00 | Kiley Eble (9 ), Agnes Mirando (10), Monica Flocken (11), Macie McNichols (10) | Gilmour Academy | Gates Mills | OH |
| 74 | 1:37.01 | Mary Ashby (9 ), Allison Stevens (10), Sam Flower (11), Meghan Bicomong (11) | Campolindo High School | Moraga | CA |
| 74 | 1:37.01 | Jenna Haufler (12), Erin Schoenfeld (10), Elise Goetzl (10), Megan Howard (11) | Miramonte High School | Orinda | CA |
| 76 | 1:37.03 | Jasmine Tosky (12), Jayna Wittenbrink (10), Shannon Scheel (12), Margaret Wenzlau (12) | Palo Alto High School | Palo Alto | CA |
| 77 | 1:37.05 | Nora McCullagh (9 ), Maria Sheridan (10), Christine Sullivan (10), Lauren Rhodes (10) | Charlotte Catholic High School | Charlotte | NC |
| 77 | 1:37.05 | Maria Villa (12), Erin Stevens (10), Lauren Gunn (12), Emily Sejna (12) | Naperville Central High School | Naperville | IL |
| 79 | 1:37.07 | Alexis Martinez (10), Summer Spradley (9 ), Hannah Calton (11), Sophie Webber (12) | Granite Bay High School | Granite Bay | CA |
| 79 | 1:37.07 | Kelly Denkler (11), Shelby Shelton (12), Alexandra Buscher (9 ), Eliza Bornman (12) | Memorial High School | Houston | TX |
| 81 | 1:37.08 | Emma Curtis (10), Emma King (9 ), Madison Stout (10), Jessie Schieman (10) | Centerville High School | Centerville | OH |
| 81 | 1:37.08 | Laura Duncan (11), Megan Hawk (10), Haley Fisher (9 ), Sammi Miller (12) | Fremont Ross High School | Fremont | OH |
| 83 | 1:37.09 | Cami Luhrs (12), Lexie Lupton (9 ), Madeline Hazle (11), Leah Pfitzer (11) | Kingwood High School | Kingwood | TX |
| 84 | 1:37.14 | Lindsay Pavich (12), Kerry Grosshans (11), Jen Coady (12), Genna Heidkamp (12) | Hinsdale Central High School | Hinsdale | IL |
| 85 | 1:37.21 | Leah Ortiz (12), Victoria Karker (10), Karoline Eckhart (11), Kathryn Eckhart (12) | Cy Creek High School | Houston | TX |
| 86 | 1:37.24 | Lindsey Schmidt (12), Casey O'Neill (9 ), Emily Houser (11), Carolyn Meier (12) | Upper Dublin High School | Ft. Washington | PA |
| 87 | 1:37.26 | Jessie Ho (11), Florina Yang (10), Joyce Zhou (11), Courtney Batcheller (10) | Saint Francis High School | Mountain View | CA |
| 88 | 1:37.28 | Myra Retrum (12), Mackenzie Coles (11), Rachael Sollman (11), Kristin VanDeventer (12) | Columbus North High School | Columbus | IN |
| 89 | 1:37.29 | Beth Grazio (10), Gentle Marissa (11), Blass Dani (12), Katie Saloky (11) | Central Columbia High School | Bloomsburg | PA |
| 89 | 1:37.29 | Lauren Mitchell (9 ), Samantha Park (11), Madison Meyer (12), Tommie Dillione (10) | Council Rock North High School | Newtown | PA |
| 91 | 1:37.30 | Emma Cammann (11), Ashlee Korsberg (11), Margaret D'Innocenzo (11), Rachel Moore (12) | Andover High School | Andover | MA |
| 92 | 1:37.35 | Christina Park (11), Abigal Rosenberg (9 ), Stephanie Horwitz (12), Molly Orbon (11) | Glenbrook North High School | Northbrook | IL |
| 93 | 1:37.36 | Dina Rommel (10), Rebecca Anthone (10), Claire Willis (11), Sydney Modeas (10) | Clarence High School | Clarence | NY |
| 93 | 1:37.36 | Morgan Contino (11), Molly Hazelbaker (12), Shay Spelman (10), Valerie Borger (12) | Turpin High School | Cincinnati | OH |
| 95 | 1:37.42 | Lexie Beemer (12), Johanna Buschhaus (11), Meghan Knox (12), Kimberly Graziano (11) | Ann Arbor Skyline High School | Ann Arbor | MI |
| 96 | 1:37.43 | Brittany Robinson (12), Sarah Rozow (12), Sarah Steele (12), Breanna Robinson (9 ) | Wawesee High School | Syracuse | IN |
| 97 | 1:37.45 | Annie Tamblyn (12), Jessa Gargan (10), Jamie Hibbs (11), Anna Demonte (12) | Ann Arbor Huron High School | Ann Arbor | MI |
| 98 | 1:37.50 | Megan Reeves (10), Julianne Kurke (12), Megan Yang (10), Morgan Fleming (9 ) | Parkview High School | Lilburn | GA |
| 99 | 1:37.52 | Allison Kirkby (11), Amber Zimmerman (9 ), Grace Wydeven (10), Abby Mack (10) | Conestoga High School | Berwyn | PA |
| 99 | 1:37.52 | Taylor Bass (10), Lauren Kalwasinski (11), Taylor Emery (10), Allison Schumacher (11) | Rochester John Marshall High School | Rochester | MN |