| Place | Time | Swimmers (Yrs) | School | City | State |
| 1 | 1:41.01 | Courtney Bartholomew (12), Melissa Vandermeulen (11), Taylor Garcia (9 ), Holly Morren (10) | Holland High School | Holland | MI |
| 2 | 1:42.36 | Jenny Barcellona (11), Heidi Poppe (10), Marissa Neel (12), Cailey Steffens (9 ) | San Ramon Valley High School | Danville | CA |
| 3 | 1:42.50 | Maggie Sheean (10), Haley Wickham (11), Mimi Schneider (10), Maureen Barron (12) | Fenwick High School | Oak Park | IL |
| 4 | 1:42.51 | Emily Slabe (10), Bridget Blood (11), Corinne Jenkins (12), Temarie Tomley (10) | Ursuline Academy | Cincinnati | OH |
| 5 | 1:42.79 | Madison White (11), Madeline Benavidez (12), Christina Ray (12), Chelsea Chenault (11) | Carondelet High School | Concord | CA |
| 6 | 1:43.04 | Lauren Driscoll (12), Lindsey McKnight (12), Alina Schulhofer (11), Jessica Randolph (11) | American Heritage School | Plantation | FL |
| 7 | 1:43.32 | Lacey Locke (11), Lucy Kramer (12), Hanna House (10), Arianna Bullard (11) | Carmel High School | Carmel | IN |
| 8 | 1:43.40 | Emily Baturka (12), Rachel Repke (11), Emily Rhodes (10), Tara O'Prey (11) | Episcopal Academy | Newtown Square | PA |
| 8 | 1:43.40 | Olivia Smoliga (11), Hallie Hengels (12), Kimmy Durkin (11), Katie Wells (9 ) | Glenbrook South High School | Glenview | IL |
| 10 | 1:43.81 | Cheyenne Low (11), Margaret Burton (11), Annie Tran (10), Margaret Hofstadter (12) | University High School | Irvine | CA |
| 11 | 1:43.88 | Jessica Sutherland (11), Riley Hayward (11), Samantha James (12), Taylor Patterson (11) | New Trier High School | Winnetka | IL |
| 12 | 1:43.89 | Soapes Kandes (9 ), Neidigh Ashley (11), Savannah Fernandez (12), Kasey Schmidt (9 ) | The Bolles School | Jacksonville | FL |
| 13 | 1:43.92 | Hannah Peiffer (11), Mikaya Reynolds (11), Ashley Yearwood (12), Kristen Vredeveld (11) | The Baylor School | Chattanooga | TN |
| 14 | 1:44.10 | Brittany Gilbert (12), Emily Wimmer (11), Nikki Smith (9 ), Mary Beth Blocher (10) | Munster High School | Munster | IN |
| 15 | 1:44.17 | Leah Goldman (10), Madison Gebhard (12), Naomi Thomas (12), Kristen Brennand (12) | Burlingame High School | Burlingame | CA |
| 16 | 1:44.22 | Lyndsie Gibson (12), Elise Weisert (12), Sydney Wheeler (9 ), Taylor Derevyanik (12) | Southlake Carroll High School | Southlake | TX |
| 17 | 1:44.29 | Alexandra Rockett (10), Kylie Reiter (12), Erin Timochenko (12), Amber Cortazzo (10) | Wilson High School | West Lawn | PA |
| 18 | 1:44.66 | Tara Halsted (11), Kathleen Benjamin (10), Beverly Nguyen (12), Alex Rieger (10) | Davis Senior High School | Davis | CA |
| 19 | 1:44.70 | Allie Pennetti (12), Gabrielle Borza (11), Erin Bishop (12), Arielle Krigger (11) | North Allegheny High School | Wexford | PA |
| 20 | 1:44.84 | Kate Canfield (9 ), Molly Coonce (12), Sarah Sykstus (12), Katherine Hare (12) | Rosary High School | Aurora | IL |
| 21 | 1:44.87 | Lauren Church (12), Katie Evans (10), Morgan Karetnick (12), Madeleine Dunn (11) | Greenwich High School | Greenwich | CT |
| 21 | 1:44.87 | Molly Hazelbaker (12), Sam Hardewig (12), Morgan Contino (11), Valerie Borger (12) | Turpin High School | Cincinnati | OH |
| 23 | 1:44.90 | Brittany Dudzinski (11), Emily Schmitt (12), Natalie Johnsen (12), Victoria Shaffer (9 ) | Upper St. Clair High School | Upper St. Clair | PA |
| 24 | 1:44.99 | Lauren Comer (12), Elizabeth Long (12), Claire Van Fossen (12), Grace Long (9 ) | Upper Arlington High School | Upper Arlington | OH |
| 25 | 1:45.02 | Emily Campbell (12), Samantha Guay (10), Bailey Nero (9 ), Rhianna Williams (11) | Fossil Ridge High School | Fort Collins | CO |
| 26 | 1:45.10 | Aly Tetzloff (9 ), Hannah Raspopovich (12), Holly Schuster (10), Rachel Bukowski (10) | Crown Point High School | Crown Point | IN |
| 27 | 1:45.14 | Caitlyn Forman (12), Olivia Kuykendall (10), Julia Johnson (11), Mackenzie Margroum (11) | Notre Dame Academy | Park Hills | KY |
| 28 | 1:45.16 | Marissa Cominelli (11), Elizabeth Aronoff (12), Carrie Bencic (11), Caroline Vexler (11) | Hawken School | Gates Mills | OH |
| 29 | 1:45.22 | Madeleine Jardeleza (11), Michelle Garland (12), Maria Jardeleza (9 ), Evan Swenson (11) | Loyola Academy | Wilmette | IL |
| 30 | 1:45.37 | Kelly McGowan (9 ), Annie Lazor (12), Hanna Pfershy (10), Beth Moran (11) | Birmingham Groves High School | Beverly Hills | MI |
| 31 | 1:45.42 | Emma Szczupakiewicz (12), Sami Pochowski (12), Megan Liebl (11), Kristen Kaiser (11) | Muskego High School | Muskego | WI |
| 32 | 1:45.43 | Natalie Dowzicky (10), Madeline Maher (12), Carolyn Yang (10), Hayli Randolph (10) | Germantown Academy | Fort Washington | PA |
| 33 | 1:45.47 | Alexandra DeLoof (12), Megan Brooks (12), Gabrielle DeLoof (10), Catherine DeLoof (9 ) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 34 | 1:45.51 | Kali Edsall (10), Annie Lane (10), Kacy Edsall (12), Mackenzie Lingle (11) | William Byrd High School | Vinton | VA |
| 35 | 1:45.54 | Konnor Rist (12), Peyton Greenburg (9 ), Emily Holt (12), Jordan Caravona (11) | duPont Manual High School | Louisville | KY |
| 36 | 1:45.57 | Nina Tremols (11), Natalie Martin (12), Holly Criscuolo (12), Sofia Revilak (9 ) | Battlefield High School | Haymarket | VA |
| 37 | 1:45.65 | Madeleine Eden (11), Olivia Anderson (9 ), Paige Haller (12), Maryellen Campbell (12) | Edina High School | Edina | MN |
| 38 | 1:45.77 | Sarah Lohman (12), Caroline Weigand (10), Bailey Pressey (11), Chelsey Reber (12) | Homestead High School | Fort Wayne | IN |
| 39 | 1:45.78 | Alexa Clayfield (10), Genevieve Bradford (12), Daniela Georges (9 ), Da Yae Choi (12) | Woodbridge High School | Irvine | CA |
| 40 | 1:45.81 | Gia Dalesandro (11), Courtney Taylor (12), Brooke Bedford (11), Maggie Maxstadt (12) | Neuqua Valley High School | Naperville | IL |
| 41 | 1:45.92 | Abby Stone (9 ), Kassandra Mestemaker (10), Lori Davis (12), Sydney King (10) | Granville High School | Granville | OH |
| 42 | 1:45.97 | Maddy Frost (11), Sammy Richart (12), Emily Lau (11), Cristina Czyrka (11) | Saline High School | Saline | MI |
| 43 | 1:45.98 | Ashley Leclair (10), Miranda Mercke (12), Victoria Horsman (9 ), Madison Lichtefeld (10) | Sacred Heart Academy | Louisville | KY |
| 44 | 1:46.03 | Hayden Walker (11), Christina Valenzuela (10), Talie Anchustegui (12), Madeline Wells (11) | Xavier College Preparatory | Phoenix | AZ |
| 45 | 1:46.05 | Kendese Nangle (12), Laura Morley (9 ), Emily Schroeder (9 ), Molly Mucciarone (12) | The Peddie School | Hightstown | NJ |
| 45 | 1:46.05 | Alexandria Kitchens (11), Ally Dupay (10), Heather Merritt (10), Emily Allen (9 ) | William A. Hough High School | Cornelius | NC |
| 47 | 1:46.11 | Kaylee Witkiewicz (9 ), Maren Reeder (11), Julia Valentine (10), Katie Allison (12) | Thomas Worthington High School | Worthington | OH |
| 48 | 1:46.16 | Maddie Bro (12), Sydney Sorenson (10), Lauren Rosser (12), Taylor Flummerfelt (11) | Ames High School | Ames | IA |
| 49 | 1:46.19 | Reanna Dona (12), Maxine Clifford (12), Sara Bertram (10), Hellen Moffitt (11) | West Potomac High School | Alexandria | VA |
| 50 | 1:46.27 | Briana Schrage (12), Molly Washko (10), Madison Kawiecki (12), Gabi D'Abato (10) | Strongsville High School | Strongsville | OH |
| 51 | 1:46.28 | Lauren Day (11), Madeline Hazle (11), Leah Pfitzer (11), Jamie Friderichs (12) | Kingwood High School | Kingwood | TX |
| 52 | 1:46.31 | Hannah Lillioja (12), Lindsay Pavich (12), Genna Heidkamp (12), Jen Coady (12) | Hinsdale Central High School | Hinsdale | IL |
| 53 | 1:46.33 | Morgan Bui (11), Rachel Rogers (12), Leah Rogers (9 ), Rachel Ward (9 ) | W.T.Woodson High School | Fairfax | VA |
| 54 | 1:46.37 | Haley Fisher (9 ), Laura Duncan (11), Sammi Miller (12), Megan Hawk (10) | Fremont Ross High School | Fremont | OH |
| 55 | 1:46.40 | Annie Tamblyn (12), Anna Demonte (12), Samadhi Kiridena (10), Jessa Gargan (11) | Ann Arbor Huron High School | Ann Arbor | MI |
| 55 | 1:46.40 | Samantha Gowdy (9 ), Erika McCormick (12), Kelsey Turner (11), Amy Steele (12) | Mt. Vernon High School | Mt. Vernon | IN |
| 57 | 1:46.47 | Lizzy Baertlein (10), Addison Skogman (9 ), Kayla Skenandore (12), Kaersten Meitz (10) | Waukesha South High School | Waukesha | WI |
| 58 | 1:46.52 | Bianca Tocci (12), Maddie Pierce (12), Julimar Avila (9 ), Victoria Bryson (10) | Weston High School | Weston | MA |
| 59 | 1:46.53 | Missy Franklin (11), Marielle Renehan (11), Sydney Cutler (9 ), Taylor Wilson (9 ) | Regis Jesuit High School | Aurora | CO |
| 60 | 1:46.56 | Maria Sheridan (10), Ali McDonough (11), Elsa Welshofer (10), Ashlyn Koletic (12) | Charlotte Catholic High School | Charlotte | NC |
| 61 | 1:46.57 | Elliott Schinella (10), Madeline Loniewski (9 ), Annie Valentine (11), Roxanne Griffore (9 ) | Mercy High School | Farmington Hills | MI |
| 61 | 1:46.57 | Kelsey Gariepy (12), Natalie Pierce (10), Mykala Arnold (9 ), Emily Parsons (12) | Seaholm High School | Birmingham | MI |
| 61 | 1:46.57 | Margaret Cooke (11), Catherine Rash (12), Alexandra Ford (10), Alexandria Sorensen (12) | The Woodlands High School | The Woodlands | TX |
| 64 | 1:46.60 | Hannah Moore (10), Lauren Steagall (12), Maddie Massey (10), Erin Mahoney (12) | Green Hope High School | Cary | NC |
| 65 | 1:46.61 | Meghan Mendez (12), Breanna Molinaro (11), Mackenzie Meixner (12), Kaitlyn Ruffing (11) | Franklin Regional High School | Murrysville | PA |
| 66 | 1:46.70 | Anika Apostalon (11), Eileen Kawamoto (11), Lexi Davis (12), Gillian St. John (9 ) | Albuquerque Academy | Albuquerque | NM |
| 67 | 1:46.71 | Ariana Waitkavicz (11), Vivian Tafuto (9 ), Samantha Surfus (11), Carly Deliberty (11) | Hershey High School | Hershey | PA |
| 68 | 1:46.74 | Summer Schmitt (11), Kathryn Coughlin (11), Lexy McCauley (11), Maggie Fletcher (11) | Christian Brothers Academy | Syracuse | NY |
| 69 | 1:46.81 | Megan Bestor (10), Ellen Johnson (12), Ellie Sills (11), Amanda Ray (12) | Lambert High School | Suwanee | GA |
| 70 | 1:46.83 | Natalie Tang (11), Kortney Kay (11), Danielle Taylor (9 ), Marissa Bergh (11) | Dougherty Valley High School | San Ramon | CA |
| 70 | 1:46.83 | Devin Madsen (12), Kate Didion (9 ), Kelsey Leonard (11), Bittany Heng (10) | Las Lomas High School | Walnut Creek | CA |
| 72 | 1:46.89 | Katie Madden (9 ), Nicole Brunozzi (11), Beth Grazio (10), Katie Saloky (11) | Central Columbia High School | Bloomsburg | PA |
| 73 | 1:46.91 | Janet Hu (10), Melissa Shebat (11), Gillian Crews (11), Ashley Doran (12) | Oakton High School | Vienna | VA |
| 74 | 1:46.95 | Nicole Dayhoff (12), Lilly Nunnally (11), Nicole Stafford (11), Erika Redding (11) | Westminster Schools | Atlanta | GA |
| 75 | 1:46.96 | Shannon Babcock (11), Laura Day (12), Frida Berggren (11), Olga Britton (10) | Lewis-Palmer High School | Monument | CO |
| 76 | 1:46.97 | Danielle Yoon (11), Briana Burns (10), Alexandra Buscher (9 ), Eliza Bornman (12) | Memorial High School | Houston | TX |
| 77 | 1:46.98 | Michelle Law (11), Rachel Prorok (11), Jen Law (12), Becca Bogle (11) | Naperville North High School | Naperville | IL |
| 78 | 1:46.99 | Kassandra Brisch (12), Shelly Patton (12), Samantha Chacon (10), Maddie McGuire (9 ) | Heritage High School | Littleton | CO |
| 79 | 1:47.01 | Cora Meehan (12), Heather Hayes (11), Katie Horn (11), Claire Brolsma (12) | Noblesville High School | Noblesville | IN |
| 80 | 1:47.02 | Anne Boodt (9 ), Lindsey Englestead (11), Olivia Barker (11), Catie Miller (10) | Bishop Gorman High School | Las Vegas | NV |
| 81 | 1:47.04 | Julia Box (9 ), Kourtney Fosse (12), Berkley Gamble (12), Zoe Verhoeven (12) | Fairview High School | Boulder | CO |
| 82 | 1:47.07 | Colleen VandePoel (11), Chelsea Rish (11), Anna Battistello (11), Caroline Fender (11) | Holland West Ottawa High School | Holland | MI |
| 83 | 1:47.11 | Maddie Miller (12), Olivia Kabacinski (12), Erin Socha (10), Madisyn Coudriet (10) | Chesterton High School | Chesterton | IN |
| 83 | 1:47.11 | Davina Jaynes (11), Hannah Arnold (10), Kayla Aitken (11), Megan Strickland (10) | Churchill High School | San Antonio | TX |
| 85 | 1:47.13 | Caroline Grasso (11), Jessica Extine (11), Lauren Elston (9 ), Larah Beaver (12) | Yorktown High School | Yorktown | IN |
| 86 | 1:47.14 | Hannah McDowell (10), Emily Wulff (10), Sydney Lloyd (10), Erin Newman (11) | Oswego East High School | Oswego | IL |
| 87 | 1:47.22 | Sarah Elderkin (11), Andi Scarcello (11), Katie Kinnear (12), Maria Volodkevich (11) | Skyline High School | Sammamish | WA |
| 88 | 1:47.27 | Stephanie Moriarty (10), Kaylin Moss (12), Caroline O'Brien (11), Kristen Moss (10) | Wilton High School | Wilton | CT |
| 89 | 1:47.29 | Courtney Mudd (11), Gabrielle Mazero (10), Caroline Thomas (9 ), Hannah Henry (11) | Hamilton Southeastern High School | Fishers | IN |
| 90 | 1:47.40 | Dorothy Ren (12), Ritty Zhai (12), Charlotte Ruby (12), Mackenzie Chock (11) | Archbishop Mitty High School | San Jose | CA |
| 91 | 1:47.49 | Megan Purdy (11), Natalie Grothe (10), Rachael Grothe (10), Alex Hubel (9 ) | Boulder City High School | Boulder City | NV |
| 92 | 1:47.57 | Elizabeth Hartell (11), Taylor Dessler (10), Alexandra Yaeger (11), Monica Banasikowski (10) | Prior Lake High School | Savage | MN |
| 93 | 1:47.61 | Sidney Cooke (11), Linda Lu (11), Sara Nicolosi (10), Mackenzie Milham (11) | Monte Vista High School | Danville | CA |
| 94 | 1:47.62 | Alex Cleveland (10), Amanda Short (11), Taite Kitchel (9 ), Grace Einterz (11) | Zionsville Community High School | Zionsville | IN |
| 95 | 1:47.64 | Pure Maleenont (9 ), Rebecca Titterton (9 ), Hannah Katz (10), Anna Strzempko (11) | Suffield Academy | Suffield | CT |
| 96 | 1:47.68 | Meredith Bell (12), Ashleigh Shanley (11), Rebecca Dickey (11), Kimberly Graziano (11) | Ann Arbor Skyline High School | Ann Arbor | MI |
| 97 | 1:47.71 | Catherine Kaucic (11), Grace Matuszyk (11), Margaret Jordan (12), Kina Zhou (11) | Niskayuna High School | Niskayuna | NY |
| 97 | 1:47.71 | Anna Tait (12), Julianne Kurke (12), Megan Yang (10), Morgan Fleming (9 ) | Parkview High School | Lilburn | GA |
| 99 | 1:47.72 | Kristina Domaschofsky (9 ), Vanessa Duran (11), Christina Alvarado (12), Mary Margaret Soderberg (11) | Ronald Reagan High School | San Antonio | TX |
| 100 | 1:47.74 | Melissa Clausen (10), Rachel Munson (9 ), Ellen Stello (11), Kristin Malone (10) | Shorewood High School | Shorewood | WI |