| Place | Time | Swimmers (Yrs) | School | City | State |
| 1 | 3:22.99 | Kristen Vredeveld (11), Bria Deveaux (12), Kimberlee John-Williams (11), Ashley Yearwood (12) | The Baylor School | Chattanooga | TN |
| 2 | 3:23.06 | Rachael Acker (12), Julia Ama (12), Casey Lincoln (12), Jenna Campbell (9 ) | Gunn High School | Palo Alto | CA |
| 3 | 3:23.94 | Lauren Driscoll (12), Jessica Randolph (11), Alina Schulhofer (11), Lindsey McKnight (12) | American Heritage School | Plantation | FL |
| 4 | 3:24.24 | Grace Long (9 ), Lauren Comer (12), Jennifer Smith (10), Gabrielle Veri (12) | Upper Arlington High School | Upper Arlington | OH |
| 5 | 3:24.46 | Holly Morren (10), Taylor Garcia (9 ), Klare Northuis (12), Courtney Bartholomew (12) | Holland High School | Holland | MI |
| 6 | 3:25.00 | Rachel Zilinskas (11), Shannon McKernan (9 ), Carolyn Yang (10), Meagan Dollard (10) | Germantown Academy | Fort Washington | PA |
| 7 | 3:25.22 | Paulina Kaminski (10), Haley Wickham (11), Maureen Barron (12), Mimi Schneider (10) | Fenwick High School | Oak Park | IL |
| 8 | 3:25.44 | Kaitlyn Albertoli (9 ), Brittany Kahn (9 ), Rebecca Millard (11), Katie McLaughlin (9 ) | JSerra Catholic High School | San Juan Capistrano | CA |
| 9 | 3:25.69 | Kathryn O'Brien (11), Heather Merritt (10), Payton Schrum (12), Emily Allen (9 ) | William A. Hough High School | Cornelius | NC |
| 10 | 3:25.87 | Margaret Ramsey (12), Hanna House (10), Alexis Bullard (12), Lacey Locke (11) | Carmel High School | Carmel | IN |
| 10 | 3:25.87 | Madelyn Veith (10), Alexis Grier (12), Carly Deliberty (11), Meaghan Raab (10) | Hershey High School | Hershey | PA |
| 12 | 3:25.99 | Chelsea Chenault (11), Nicole Palmer (12), Natalie Amberg (10), Madison White (11) | Carondelet High School | Concord | CA |
| 13 | 3:26.08 | Emma Henderson (11), Annie Tran (10), Megan Rankin (12), Cheyenne Low (11) | University High School | Irvine | CA |
| 14 | 3:26.11 | Campbell Costley (11), Morgan Scott (12), Stephanie Marchuk (11), Samantha James (12) | New Trier High School | Winnetka | IL |
| 15 | 3:26.87 | Elaine Powell (11), Colleen Wixted (11), Fiona Kane (12), Bonnie Brandon (12) | Cherry Creek High School | Greenwood Village | CO |
| 16 | 3:26.92 | Hannah Mitchell (10), Lizzy Lyon (12), Taite Kitchel (9 ), Zoe Mattingly (12) | Zionsville Community High School | Zionsville | IN |
| 17 | 3:27.13 | Rachel Astles (12), Maddie Downs (10), Katie Christy (9 ), Nicole Stafford (11) | Westminster Schools | Atlanta | GA |
| 18 | 3:27.20 | Emily Campbell (12), Riley Hoffman (9 ), Bailey Nero (9 ), Rhianna Williams (11) | Fossil Ridge High School | Fort Collins | CO |
| 19 | 3:27.39 | Allison Brown (12), Ana Henderson (9 ), Paige Matherson (10), Marissa Neel (12) | San Ramon Valley High School | Danville | CA |
| 20 | 3:27.47 | Camilla Czulada (12), Taylor Brew (12), Amber Cortazzo (10), Alexandra Rockett (10) | Wilson High School | West Lawn | PA |
| 21 | 3:27.63 | Linnea Mack (11), Sierra Painter (9 ), Sandra Soe (9 ), Michelle Berry (12) | Pioneer High School | San Jose | CA |
| 21 | 3:27.63 | Reia Tong (12), Victoria Kuhn (12), Audrey Gould (11), Charlotte Meyer (11) | Walt Whitman High School | Bethesda | MD |
| 23 | 3:27.65 | Michaela Rookus (12), Audra Thornton (12), Makayla Myers (10), Danielle Freeman (11) | Hudsonville High School | Hudsonville | MI |
| 24 | 3:27.79 | Jasmine Tosky (12), Jayna Wittenbrink (10), Haley Mortenson (11), Molly Zebker (11) | Palo Alto High School | Palo Alto | CA |
| 24 | 3:27.79 | Selby Sturzenegger (10), Erica Myers (11), Erin Sheridan (12), Ally Howe (10) | Sacred Heart Preparatory | Atherton | CA |
| 26 | 3:27.80 | Abby Wu (11), Emily Slabe (10), Alisabeth Marsteller (10), Bridget Blood (11) | Ursuline Academy | Cincinnati | OH |
| 27 | 3:27.81 | Madison Taylor (11), Clare Nienstedt (10), Mollie Pulte (9 ), Jaynie Pulte (12) | Marian High School | Bloomfield Hills | MI |
| 28 | 3:27.99 | Lindsay Schultz (11), Emily Zapinski (11), Rebecca Upton (10), Lyndsie Gibson (12) | Southlake Carroll High School | Southlake | TX |
| 29 | 3:28.39 | Claire Rushin (9 ), Madeleine Jardeleza (11), Evan Swenson (11), Mallory Remick (12) | Loyola Academy | Wilmette | IL |
| 30 | 3:28.44 | Alexandria Franz (12), Taylor Yamahata (10), Hailey Ferko (12), Cathy Woo (11) | Rio Americano High School | Sacramento | CA |
| 31 | 3:28.52 | Sarah Koucheki (11), Carrie Bencic (11), Jordan Bitterman (12), Morgan Cohara (12) | Hawken School | Gates Mills | OH |
| 32 | 3:28.56 | Tara Halsted (11), Marissa LaFreniere (11), Rachel Johnson (12), Beverly Nguyen (12) | Davis Senior High School | Davis | CA |
| 33 | 3:28.60 | Emma Wittmer (9 ), Amelia Anderson (9 ), Madeleine Eden (11), Nikki Larson (12) | Edina High School | Edina | MN |
| 34 | 3:28.61 | Abby Jagdfeld (10), Molly Manchon (9 ), Natalie Clausen (9 ), Sara Zemanovic (12) | Waukesha South High School | Waukesha | WI |
| 35 | 3:28.63 | Gabrielle DeLoof (10), Cassandra Morse (10), Alexandra DeLoof (12), Catherine DeLoof (9 ) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 36 | 3:28.68 | Nora McCullagh (9 ), Ashlyn Koletic (12), Elsa Welshofer (10), Lauren Rhodes (10) | Charlotte Catholic High School | Charlotte | NC |
| 37 | 3:28.77 | Ashlee Korsberg (11), Alexa Korsberg (11), Margaret D'Innocenzo (11), Rachel Moore (12) | Andover High School | Andover | MA |
| 38 | 3:28.83 | Katerina Borisov (11), Erin Bishop (12), Jade Song (10), Kally Vanderbilt (12) | North Allegheny High School | Wexford | PA |
| 39 | 3:28.84 | Kina Zhou (11), Mikayla Frodey (10), Emma Terwilliger (12), Margaret Jordan (12) | Niskayuna High School | Niskayuna | NY |
| 40 | 3:28.88 | Kate Curley (11), Julia Campbell (11), Maddie Miller (12), Olivia Kabacinski (12) | Chesterton High School | Chesterton | IN |
| 41 | 3:29.00 | Kirsten Brand (11), Rachel Knowles (9 ), Eva Chung (9 ), Iris Brand (9 ) | Amador Valley High School | Pleasanton | CA |
| 41 | 3:29.00 | Kylie Boyce (11), Devin Newton (10), Alys Williams (12), Ellie Morrissey (12) | Edison High School | Huntington Beach | CA |
| 43 | 3:29.25 | Ashlee Linn (12), Nancy Hu (9 ), Lexy Raybon (10), Taylor Katz (11) | Riverview High School | Sarasota | FL |
| 44 | 3:29.28 | Bethany Galat (10), Emily Meckstroth (9 ), Kailin Potter (12), Sarah Coyne (12) | Penn High School | Mishawaka | IN |
| 45 | 3:29.35 | Claire Liu (11), Carly Catella (10), Kristin Connors (12), Henriette Stenkvist (12) | Panther Creek High School | Cary | NC |
| 46 | 3:29.48 | Kelsey Turner (11), Samantha Gowdy (9 ), Clara Baggett (9 ), Mary Klueh (12) | Mt. Vernon High School | Mt. Vernon | IN |
| 47 | 3:29.56 | Sami Pochowski (12), Emma Szczupakiewicz (12), Megan Liebl (11), Kristen Kaiser (11) | Muskego High School | Muskego | WI |
| 48 | 3:29.65 | Allison Stevens (10), Mary Ashby (9 ), Hannah Grubbs (10), Meghan Bicomong (11) | Campolindo High School | Moraga | CA |
| 49 | 3:29.70 | Gabriele Serniute (9 ), Maddy Sims (10), Hannah Kaufmann (11), Gabby Sims (10) | Downers Grove North High School | Downers Grove | IL |
| 50 | 3:29.71 | Megan Purdy (11), Rachael Grothe (10), Cori Molisee (10), Alex Hubel (9 ) | Boulder City High School | Boulder City | NV |
| 51 | 3:29.72 | Lauren Day (11), Leah Pfitzer (11), Madeline Hazle (11), Jamie Friderichs (12) | Kingwood High School | Kingwood | TX |
| 52 | 3:29.83 | Erin Falconer (9 ), Amy Klink (12), Jennifer Kordik (9 ), Karrie Kozokar (10) | Mundelein High School | Mundelein | IL |
| 53 | 3:29.86 | Sarah Gibson (11), Mary Margaret Soderberg (11), Kristina Domaschofsky (9 ), Colleen Konetzke (12) | Ronald Reagan High School | San Antonio | TX |
| 54 | 3:29.91 | Maddy Frost (11), Kate Rogers (10), Melanie Schroeder (11), Nikki Flynn (12) | Saline High School | Saline | MI |
| 55 | 3:29.92 | Sarah Love (11), Kim Zehnder (9 ), Elizabeth House (11), Konnor Rist (12) | duPont Manual High School | Louisville | KY |
| 56 | 3:30.00 | Dina Rommel (10), Rebecca Anthone (10), Victoria Butler (10), Sydney Modeas (10) | Clarence High School | Clarence | NY |
| 57 | 3:30.01 | Carolyn Kane (11), Jenine Einhorn (12), Amelia Schilling (11), Isabel Wyer (10) | Minnetonka High School | Minnetonka | MN |
| 58 | 3:30.06 | Mika Hlavin (12), Olivia Campbell (12), Eleanor Meeks (9 ), Rachel Stoehr (12) | Lake Forest High School | Lake Forest | IL |
| 59 | 3:30.08 | Leah Ortiz (12), Victoria Karker (10), Karoline Eckhart (11), Kathryn Eckhart (12) | Cy Creek High School | Houston | TX |
| 60 | 3:30.17 | Morgan Karetnick (12), Samantha Hill (12), Kimberly Hill (10), Lauren Church (12) | Greenwich High School | Greenwich | CT |
| 61 | 3:30.28 | Kimmie Kreuzberger (12), Alexis Lee (12), Aislinn Dresel (10), Alexandra Thomason (12) | Marin Catholic High School | Kentfield | CA |
| 62 | 3:30.34 | Erica Reifinger (12), Erika Freeman (9 ), Megan Polaha (11), Emily Stang (12) | Parkland High School | Allentown | PA |
| 63 | 3:30.36 | Brittany Gilbert (12), Joann Wakefield (12), Jillian Homans (12), Mary Beth Blocher (10) | Munster High School | Munster | IN |
| 64 | 3:30.37 | Julimar Avila (9 ), Victoria Bryson (10), Maddie Pierce (12), Bianca Tocci (12) | Weston High School | Weston | MA |
| 65 | 3:30.39 | Riley Schroedter (11), Megan Childs (11), Tara Blanzy (12), Gia Dalesandro (11) | Neuqua Valley High School | Naperville | IL |
| 66 | 3:30.55 | Camey Rabold (12), Michele Rielly (12), Aly Francis (12), Samantha Schuttinger (11) | Bishop Watterson High School | Columbus | OH |
| 67 | 3:30.58 | Katie Miller (11), Natalie O' Brien (10), Emily O' Brien (12), Jillian Stoneburg (12) | Akron Firestone High School | Akron | OH |
| 67 | 3:30.58 | Louisa Hoffman (11), Kristine Rosenberger (11), Jennifer Weigand (12), Elly Vitek (11) | Lyons Township High School | Lagrange | IL |
| 69 | 3:30.66 | Abigail Rosenberg (9 ), Colleen Doolan (9 ), Stephanie Horwitz (12), Molly Orbon (11) | Glenbrook North High School | Northbrook | IL |
| 70 | 3:30.70 | Mackenzie Meixner (12), Kaitlyn Ruffing (11), Nicole Stevenson (11), Alyssa Ruffing (10) | Franklin Regional High School | Murrysville | PA |
| 71 | 3:30.74 | Savannah Long (11), Melissa Postoll (11), Morgan Stephenson (12), Rebecca Postoll (9 ) | Kennesaw Mountain High School | Kennesaw | GA |
| 72 | 3:30.75 | Danielle Taylor (9 ), Jackie Taylor (12), Natalie Tang (11), Marissa Bergh (11) | Dougherty Valley High School | San Ramon | CA |
| 73 | 3:30.78 | Reagan Cook (10), Mariah Hamil (12), Courtney Klefeker (12), Sarah Lohman (12) | Homestead High School | Fort Wayne | IN |
| 74 | 3:30.92 | Charlotte Ruby (12), Ritty Zhai (12), Sarah Shimomura (9 ), Marie-Pierre Delisle (10) | Archbishop Mitty High School | San Jose | CA |
| 75 | 3:30.96 | Laura Branton (9 ), Stephanie Hallock (12), Kendall Lawhorn (10), Janet Hu (10) | Oakton High School | Vienna | VA |
| 75 | 3:30.96 | Molly O'Brien (11), Sarah Hitchens (11), Lauren Law (11), Julie Dobson (9 ) | Villa Maria Academy | Malvern | PA |
| 77 | 3:30.97 | Rachel Burke (12), Erin Hart (9 ), Emily Launer (12), Molly Coonce (12) | Rosary High School | Aurora | IL |
| 78 | 3:30.99 | Myra Retrum (12), Mackenzie Coles (11), Rachael Sollman (11), Kristin VanDeventer (12) | Columbus North High School | Columbus | IN |
| 79 | 3:31.11 | Sato Kakihara (12), Kathleen McGee (9 ), Roxanne Griffore (12), Olivia Samoray (12) | Mercy High School | Farmington Hills | MI |
| 80 | 3:31.12 | Kelsey Gouge (12), Evan Parker (11), Becky Bass (10), Rachel Muller (11) | Brookwood High School | Snellville | GA |
| 81 | 3:31.19 | Kelly Cordes (9 ), Kimmy Durkin (11), Hallie Hengels (12), Katie Wells (9 ) | Glenbrook South High School | Glenview | IL |
| 82 | 3:31.27 | Alicia Tiberino (10), Bridget Dromerick (10), Elaina Gu (9 ), Natalya Ares (12) | Winston Churchill High School | Potomac | MD |
| 83 | 3:31.31 | Casey O'Neill (9 ), Sam Wheatley (10), Emily Houser (11), Carolyn Meier (12) | Upper Dublin High School | Ft. Washington | PA |
| 84 | 3:31.36 | Sarah Westley (11), Erin Fife (10), Madison Takoushian (11), Bethany Dewitt (12) | Rustin High School | West Chester | PA |
| 85 | 3:31.37 | Cecelia Williams (10), Alyssa Yambor-Maul (11), Lia Lombardi (11), Delaney Barnard (10) | Lawton Chiles High School | Tallahassee | FL |
| 86 | 3:31.39 | Kaitlyn Van Peursem (11), Daniela Georges (9 ), Genevieve Bradford (12), Da Yae Choi (12) | Woodbridge High | Irvine | CA |
| 87 | 3:31.42 | Hollace Barden (12), Allison McCormick (11), Pippa Saunders (12), Brynne Wong (11) | Corona Del Mar High School | Newport Beach | CA |
| 88 | 3:31.47 | Talie Anchustegui (12), Kathleen Mulligan (11), Hayden Walker (11), Alisha Bender (12) | Xavier College Preparatory | Phoenix | AZ |
| 89 | 3:31.49 | Ritchey Howe (12), Johee Hwang (11), Jenner McLeod (11), Eliza Bragg (11) | Deerfield Academy | Deerfield | MA |
| 90 | 3:31.53 | Fotini Kaufman (12), Abbey King (12), Jen Coady (12), Genna Heidkamp (12) | Hinsdale Central High School | Hinsdale | IL |
| 91 | 3:31.64 | Morgan Contino (11), Shay Spelman (10), Valerie Borger (12), Molly Hazelbaker (12) | Turpin High School | Cincinnati | OH |
| 92 | 3:31.65 | Taylor Flummerfelt (11), Rachel Shriver (12), Hannah Newell (10), Maddie Bro (12) | Ames High School | Ames | IA |
| 93 | 3:31.68 | Natalie Johnsen (12), Jessica Reilly (11), Brittany Dudzinski (11), Victoria Shaffer (9 ) | Upper St. Clair High School | Upper St. Clair | PA |
| 94 | 3:31.74 | Emily Konishi (9 ), Brenna Thomas (11), Hanna Kruip (12), Rachel Thompson (10) | Foothill High School | Santa Ana | CA |
| 95 | 3:31.77 | Emma Siewert (12), Emily Carideo (12), Savannah Savitt (9 ), Rebecca Werdine (12) | Tampa Preparatory | Tampa | FL |
| 96 | 3:31.80 | Annie Lane (10), Kacy Edsall (12), Mackenzie Lingle (11), Kali Edsall (10) | William Byrd High School | Vinton | VA |
| 97 | 3:31.82 | Madelyn Huttner (12), Michaela Merlihan (11), Meghan Merlihan (9 ), Sarae Dydo (12) | Esperanza High School | Anaheim | CA |
| 98 | 3:31.83 | Kellie Fecarotta (12), Haley Wan (10), Sydney Franzen (9 ), Lizzie Devitt (10) | Santa Margarita Catholic High School | Rancho Santa Margarita | CA |
| 99 | 3:31.90 | Joelle Borchers (9 ), Caitlin Cooper (10), Sarah Coats (10), Kathleen Cook (10) | Woodward Academy | College Park | GA |
| 100 | 3:32.00 | Riley Marks (12), Taylor Silvestry (10), Raquel Grays (10), Brianna Higgins (11) | Johnson High School | San Antonio | TX |