| Place | Time | Swimmer (Yr) | School | City | State |
| 1 | :52.41 | Danielle Nack (11) | Mankato West High School | Mankato | MN |
| 2 | :52.78 | Jasmine Mau (11) | Punahou School | Honolulu | HI |
| 3 | :52.93 | Katie McLaughlin (10) | Jserra Catholic High School | San Juan Capistrano | CA |
| 4 | :53.35 | Sonia Wang (10) | Upland High School | Upland | CA |
| 5 | :53.44 | Gia Dalesandro (12) | Neuqua Valley High School | Naperville | IL |
| 6 | :53.46 | Ariana Saghafi (10) | Albany High School | Albany | CA |
| 7 | :53.49 | Nicole Stafford (12) | Westminster Schools | Atlanta | GA |
| 8 | :53.53 | Ella Eastin (10) | Crean Lutheran High School | Irvine | CA |
| 9 | :53.55 | Katie Grover (11) | Milton High School | Milton | GA |
| 10 | :53.62 | Kaitlyn Jones (12) | The Tatnall School | Wilmington | DE |
| 11 | :53.69 | Brittany Aoyama (12) | Linden High School | Linden | CA |
| 11 | :53.69 | Leah Goldman (11) | Burlingame High School | Burlingame | CA |
| 13 | :53.88 | Margaret Gruber (12) | Mars Area High School | Mars | PA |
| 13 | :53.88 | Linnea Mack (12) | Pioneer High School | San Jose | CA |
| 15 | :53.90 | Ariana Bullard (12) | Carmel High School | Carmel | IN |
| 16 | :54.08 | Kayla Moran (12) | Lyman High School | Longwood | FL |
| 17 | :54.11 | Niki Price (11) | Northeastern High School | Manchester | PA |
| 18 | :54.13 | Maddison Wright (10) | Waterford United High Schools | Waterford | MI |
| 19 | :54.16 | Dana Grindall (12) | Amity High School | Woodbridge | CT |
| 20 | :54.17 | Valerie Yoshimura (10) | Baldwin | Bryn Mawr | PA |
| 21 | :54.30 | Zoe Avestruz (10) | Chanhassen High School | Chanhassen | MN |
| 22 | :54.31 | Angela Algee (12) | Dr. Phillips High School | Orlando | FL |
| 23 | :54.33 | Hellen Moffitt (12) | West Potomac High School | Alexandria | VA |
| 24 | :54.36 | Mary Schneider (11) | Fenwick High School | Oak Park | IL |
| 25 | :54.38 | Katie Ledecky (10) | Stone Ridge School | Bethesda | MD |
| 26 | :54.39 | Lindsey Engel (11) | Crean Lutheran High School | Irvine | CA |
| 26 | :54.39 | Madelyn Murphy (9 ) | Carondelet High School | Concord | CA |
| 28 | :54.40 | Olivia Barker (12) | Bishop Gorman High School | Las Vegas | NV |
| 29 | :54.46 | Megan Moroney (10) | Saint Andrew's School | Boca Raton | FL |
| 30 | :54.50 | Kylie Jordan (9 ) | The Madeira School | Mclean | VA |
| 31 | :54.61 | Hanna House (11) | Carmel High School | Carmel | IN |
| 31 | :54.61 | Aly Tetzloff (10) | Crown Point High School | Crown Point | IN |
| 33 | :54.62 | Carrie Johnson (10) | Mt. Juliet High School | Mt. Juliet | TN |
| 34 | :54.67 | Christina Leander (12) | West Chester East High School | West Chester | PA |
| 35 | :54.68 | Emily Eastin (9 ) | Crean Lutheran High School | Irvine | CA |
| 36 | :54.69 | Elsa Welshofer (11) | Charlotte Catholic High School | Charlotte | NC |
| 37 | :54.70 | Leah Rogers (10) | W. T. Woodson High School | Fairfax | VA |
| 38 | :54.72 | Taylor Garcia (10) | Holland High School | Holland | MI |
| 39 | :54.73 | Mackenzie Hornibrook (12) | Unionville High School | Kennett Square | PA |
| 39 | :54.73 | Bre Thorne (9 ) | Eden Prairie High School | Eden Prairie | MN |
| 41 | :54.86 | Brynne Wong (12) | Corona del Mar High School | Newport Beach | CA |
| 42 | :54.89 | Sarah Koucheki (12) | Hawken School | Gates Mills | OH |
| 43 | :54.94 | Anna Quinn (11) | Thousand Oaks High School | Thousand Oaks | CA |
| 44 | :54.99 | Michi Aisawa (12) | Adrian C. Wilcox High School | Santa Clara | CA |
| 45 | :55.00 | Katelyn Miller (12) | Akron Firestone High School | Akron | OH |
| 46 | :55.02 | Marie-Pierre Delisle (11) | Archbishop Mitty High School | San Jose | CA |
| 47 | :55.03 | Emily Slabe (11) | Ursuline Academy | Cincinnati | OH |
| 48 | :55.05 | Kristina Li (11) | Thomas S. Wootton High School | Rockville | MD |
| 49 | :55.09 | Morgan Contino (12) | Turpin High School | Cincinnati | OH |
| 49 | :55.09 | Jennifer Marrkand (11) | Westford Academy | Westford | MA |
| 51 | :55.10 | Kimberlee John-Williams (12) | The Baylor School | Chattanooga | TN |
| 52 | :55.14 | Leah Pfitzer (12) | Kingwood High School | Kingwood | TX |
| 53 | :55.17 | Kierston Farley-Sepe (9 ) | Fenwick High School | Oak Park | IL |
| 54 | :55.19 | Emily Gibson (11) | Southlake Carroll High School | Southlake | TX |
| 55 | :55.21 | Nicole Smith (10) | Munster High School | Munster | IN |
| 55 | :55.21 | Alyssa Yambor-Maul (12) | Lawton Chiles High School | Tallahassee | FL |
| 57 | :55.25 | Megan Campbell (11) | San Marin High School | Novato | CA |
| 57 | :55.25 | Emma Sougstad (12) | Mason City High School | Mason City | IA |
| 59 | :55.26 | Sydney Hirschi (12) | Loretto Academy | El Paso | TX |
| 59 | :55.26 | Vanessa Krause (9 ) | Chesterton High School | Chesterton | IN |
| 61 | :55.27 | Paige Maynard (10) | Vacaville High School | Vacaville | CA |
| 62 | :55.28 | Hilvy Cheung (12) | American High School | Fremont | CA |
| 63 | :55.29 | Amy Lubawy (11) | Bishop Gorman High School | Las Vegas | NV |
| 63 | :55.29 | Kindle Van Linge (11) | Menlo-Atherton High School | Atherton | CA |
| 65 | :55.31 | Caroline McTaggart (10) | The Holton Arms School | Bethesda | MD |
| 66 | :55.32 | Breanna Sapienza (11) | Cascade Christian High School | Medford | OR |
| 67 | :55.33 | Jayna Wittenbrink (11) | Palo Alto High School | Palo Alto | CA |
| 68 | :55.35 | Summer Campbell (11) | Paschal High School | Fort Worth | TX |
| 69 | :55.38 | Courtney Grunewald (12) | Elk River Senior High School | Elk River | MN |
| 70 | :55.39 | Tyla Martin (10) | Pine Crest School | Fort Lauderdale | FL |
| 71 | :55.44 | Stephanie Samudro (11) | Corona del Mar High School | Newport Beach | CA |
| 72 | :55.45 | Mik Ranslem (10) | William S. Hart High School | Newhall | CA |
| 73 | :55.46 | Lauren Case (9 ) | Woodstock High School | Woodstock | GA |
| 74 | :55.47 | Grace Dimeo (11) | Booker High School | Sarasota | FL |
| 75 | :55.49 | Hannah Musser (11) | Blue Valley Northwest High School | Overland Park | KS |
| 76 | :55.51 | Bailey Nero (10) | Fossil Ridge High School | Fort Collins | CO |
| 76 | :55.51 | Cara Norris (10) | Sycamore High School | Cincinnati | OH |
| 78 | :55.53 | Megan Polaha (12) | Parkland High School | Allentown | PA |
| 78 | :55.53 | Bailey Pressey (12) | Homestead High School | Fort Wayne | IN |
| 80 | :55.56 | Elizabeth Auckley (11) | Bay High School | Bay Village | OH |
| 80 | :55.56 | Reilly Lanigan (9 ) | Lake Forest High School | Lake Forest | IL |
| 82 | :55.59 | Hannah Matheson (12) | Montgomery High School | Skillman | NJ |
| 83 | :55.60 | Christina Lappin (9 ) | Lincoln Charter School | Denver | NC |
| 84 | :55.62 | Megan Bestor (11) | Lambert High School | Suwanee | GA |
| 84 | :55.62 | Christie Jensen (10) | Castle High School | Newburgh | IN |
| 86 | :55.67 | Hannelore Strash (12) | Alamo Heights High School | San Antonio | TX |
| 87 | :55.68 | Remedy Rule (10) | Western Albemarle High School | Crozet | VA |
| 87 | :55.68 | Maddie Rusch (12) | New Canaan High School | New Canaan | CT |
| 89 | :55.71 | Anna Williams (12) | Naperville North High School | Naperville | IL |
| 90 | :55.72 | Kelsey Leonard (12) | Las Lomas High School | Walnut Creek | CA |
| 91 | :55.74 | Haley Bishop (10) | Panther Creek High School | Cary | NC |
| 91 | :55.74 | Emily Rhodes (11) | The Episcopal Academy | Newtown Square | PA |
| 93 | :55.75 | Ally Howe (11) | Sacred Heart Preparatory | Atherton | CA |
| 94 | :55.76 | Morgan Bullock (9 ) | Zeeland High School | Zeeland | MI |
| 95 | :55.77 | Chanel Bonin (11) | South Lyon High School | South Lyon | MI |
| 95 | :55.77 | Julia Roller (12) | Naperville Central High School | Naperville | IL |
| 95 | :55.77 | Zoya Wahlstrom (11) | Lakeville North High School | Lakeville | MN |
| 98 | :55.79 | Hannah Grubbs (11) | Campolindo High School | Moraga | CA |
| 99 | :55.80 | Hannah Calton (12) | Granite Bay High School | Granite Bay | CA |
| 99 | :55.80 | Gianna Garcia (9 ) | San Ramon Valley High School | Danville | CA |