| Place | Time | Relay Swimmerws (Yrs) | School | City | State |
| 1 | 3:20.42 | Chelsea Chenault (12), Natalie Amberg (11), Madelyn Murphy (9 ), Madison White (12) | Carondelet High School | Concord | CA |
| 2 | 3:21.20 | Kaitlyn Albertoli (10), Karli Thuen (10), Rebecca Millard (12), Katie McLaughlin (10) | Jserra Catholic High School | San Juan Capistrano | CA |
| 3 | 3:21.63 | Shannon McKernan (10), Meagan Dollard (11), Madison Visco (10), Rachel Zilinskas (12) | Germantown Academy | Fort Washington | PA |
| 4 | 3:23.19 | Meaghan Raab (11), Carly Deliberty (12), Vivian Tafuto (10), Madelyn Veith (11) | Hershey High School | Hershey | PA |
| 4 | 3:23.19 | Temarie Tomley (11), Emily Slabe (11), Bridget Blood (12), Alisabeth Marsteller (11) | Ursuline Academy | Cincinnati | OH |
| 6 | 3:23.37 | Veronica Burchill (9 ), Hanna House (11), Claire Adams (9 ), Lacey Locke (12) | Carmel High School | Carmel | IN |
| 7 | 3:23.43 | Tasija Karosas (12), Brenna Ruth (11), Rachael Bradford-Feldman (10), Megan Moroney (10) | Saint Andrew's School | Boca Raton | FL |
| 8 | 3:23.72 | Kasey Schmidt (10), Claire Rasmus (10), Jessica Hodgson (11), Siwan Thomas-Howells (10) | The Bolles School | Jacksonville | FL |
| 9 | 3:24.50 | Olivia Smoliga (12), Bailey Moynihan (11), Kelly Cordes (10), Katie Wells (10) | Glenbrook South High School | Glenview | IL |
| 10 | 3:24.61 | Nora McCullagh (10), Nicole Emery (11), Elsa Welshofer (11), Lauren Rhodes (11) | Charlotte Catholic High School | Charlotte | NC |
| 11 | 3:25.25 | Paulina Kaminski (11), Kierston Farley-Sepe (9 ), Haley Wickham (12), Mary Schneider (12) | Fenwick High School | Oak Park | IL |
| 12 | 3:25.38 | Abby Jagdfeld (11), Molly Manchon (10), Natalie Clausen (10), Kaersten Meitz (11) | Waukesha South High School | Waukesha | WI |
| 13 | 3:25.56 | Tara Rogers (11), Olivia DiStefano (10), Eve Kosten (11), Emily Kosten (10) | North Penn High School | Lansdale | PA |
| 14 | 3:25.90 | Jessica Short (10), Natalie Johnson (12), Kathryn Mohr (10), Katie Drabot (9 ) | Cedarburg High School | Cedarburg | WI |
| 15 | 3:26.16 | Allison McCormick (12), Ioanna Haralabidis (12), Brynne Wong (12), Stephania Haralabidis (12) | Corona del Mar High School | Newport Beach | CA |
| 16 | 3:26.22 | Maddie Downs (11), Katie Christy (10), Jamie Christy (12), Nicole Stafford (12) | Westminster Schools | Atlanta | GA |
| 17 | 3:26.23 | Kaia Grobe (10), Shelby Holmes (11), Kylie Dahlgren (10), Zoe Avestruz (10) | Chanhassen High School | Chanhassen | MN |
| 18 | 3:26.51 | Alex Rieger (11), Marrisa LaFreniere (12), Chenoa Devine (9 ), Tara Halsted (12) | Davis Senior High School | Davis | CA |
| 19 | 3:26.55 | Morgan Soulia (10), Jade Song (11), Anna Seethaler (12), Jackie Du (10) | North Allegheny Senior High School | Wexford | PA |
| 20 | 3:26.70 | Grace Long (10), Elizabeth Weldon (11), Grace Van Fossen (10), Jennifer Smith (11) | Upper Arlington High School | Upper Arlington | OH |
| 21 | 3:27.05 | Sarah Kaunitz (11), Sayeh Faridnia (10), Lisa Kaunitz (9 ), Carly Reid (11) | Monta Vista High School | Cupertino | CA |
| 22 | 3:27.48 | Campbell Costley (12), Jessica Sutherland (12), Nicole Retondo (10), Stephanie Marchuk (12) | New Trier High School | Winnetka | IL |
| 23 | 3:27.58 | Heaven Quintana (9 ), Kassidy Henson (9 ), Hannah Calton (12), Lauren Fitzgerald (10) | Granite Bay High School | Granite Bay | CA |
| 24 | 3:27.66 | Ashley LeClair (11), Asia Seidt (9 ), Leah Stevens (10), Brooke Bauer (9 ) | Sacred Heart Academy | Louisville | KY |
| 25 | 3:27.68 | Mary Ashby (10), Hannah Grubbs (11), Allison Stevens (11), Meghan Bicomong (12) | Campolindo High School | Moraga | CA |
| 25 | 3:27.68 | Linnea Mack (12), Sierra Painter (10), Annalisa Perez (9 ), Sandra Soe (10) | Pioneer High School | San Jose | CA |
| 27 | 3:27.83 | Ellie Suek (12), Kira Zubar (11), Bre Thorne (9 ), Marla Albanese (10) | Eden Prairie High School | Eden Prairie | MN |
| 27 | 3:27.83 | Sydney Larson (9 ), Morgan McClure (11), Taylor Yamahata (11), Cathy Woo (12) | Rio Americano High School | Sacramento | CA |
| 29 | 3:27.86 | Amanda Moore (12), Kathryn Carson (11), Sarah Medellin (12), Lindsay Morrow (12) | Cinco Ranch High School | Katy | TX |
| 30 | 3:27.89 | Emily Konishi (10), Brooke Maxson (9 ), Courtney Mykkanen (9 ), Rachel Thompson (11) | Foothill High School | Santa Ana | CA |
| 31 | 3:28.00 | Carolyn Kane (12), Elizabeth Kane (12), Amelia Schilling (12), Isabel Wyer (11) | Minnetonka High School | Minnetonka | MN |
| 32 | 3:28.01 | Alicia Tiberino (11), Michelle Illig (12), Hannah Lindsey (9 ), Elaina Gu (10) | Winston Churchill High School | Potomac | MD |
| 33 | 3:28.16 | Davina Jaynes (12), Sophia Rodriguez (11), Haley Herzberg (9 ), Megan Strickland (11) | Churchill High School | San Antonio | TX |
| 33 | 3:28.16 | Racheal Bukowski (11), Aly Tetzloff (10), Mackenzie Artim (9 ), Holly Schuster (11) | Crown Point High School | Crown Point | IN |
| 35 | 3:28.28 | Riley Hoffman (10), Quinn Fawcett (10), Bailey Nero (10), Rhianna Williams (12) | Fossil Ridge High School | Fort Collins | CO |
| 36 | 3:28.32 | Julieyanna Parker (9 ), Lindsay Temple (11), Jacquelynn Parker (12), Rachel Stoddard (12) | Hillsborough High School | Hillsborough | NJ |
| 37 | 3:28.51 | Asia Antoniuk (12), Mik Ranslem (10), Megan Schultz (12), Tamara Santoyo (10) | William S. Hart High School | Newhall | CA |
| 38 | 3:28.54 | Maddy Sims (11), Emily Albrecht (10), Hannah Kaufmann (12), Gabby Sims (11) | Downers Grove North High School | Downers Grove | IL |
| 39 | 3:28.55 | Lexie Malazdrewicz (12), Bailey Smith (10), Myriah Ariza-Balter (12), Kamryn Holland (10) | Evergreen High School | Evergreen | CO |
| 40 | 3:28.64 | Cassie Misiewicz (11), Holly Morren (11), Anna Giesler (9 ), Taylor Garcia (10) | Holland High School | Holland | MI |
| 41 | 3:28.69 | Courtney Evensen (12), Rachael Peterson (10), Emma Ruehle (9 ), Brooke Evensen (12) | Robbinsdale Armstrong High School | Plymouth | MN |
| 42 | 3:28.72 | Dina Rommel (11), Rebecca Anthone (11), Claire Willis (12), Sydney Modeas (11) | Clarence High School | Clarence | NY |
| 43 | 3:28.78 | Taylor Katz (12), Hannah James (11), Spence Atkins (10), Niamh O'Grady (11) | Riverview High School | Sarasota | FL |
| 44 | 3:28.82 | Sarah Love (12), Elizabeth House (12), Bryn Handley (11), Kim Zehnder (10) | Du Pont Manual High School | Louisville | KY |
| 45 | 3:28.84 | Lizzie Troy (10), Gabby Marinelli (9 ), Jordan Decker (9 ), Zoe Thatcher (11) | Mason High School | Mason | OH |
| 46 | 3:28.90 | Sarah Koucheki (12), Claire Doerr (9 ), Heather Svitavsky (9 ), Carrie Bencic (12) | Hawken School | Gates Mills | OH |
| 46 | 3:28.90 | Lauren Law (12), Molly O'Brien (12), Julie Dobson (10), Sarah Hitchens (12) | Villa Maria Academy | Malvern | PA |
| 48 | 3:29.02 | Kirsten Brand (12), Caitlyn Plattel (9 ), Eva Chung (10), Iris Brand (10) | Amador Valley High School | Pleasanton | CA |
| 49 | 3:29.06 | Morgan Hill (9 ), Megan Conrad (12), Kylie Sheapp (12), Anna Kolanwoski (12) | Sherwood High School | Sandy Spring | MD |
| 50 | 3:29.32 | Maddy Nelson (11), Lindsay Craig (11), Emily Tiedemann (12), Beata Nelson (9 ) | Verona Area High School | Verona | WI |
| 51 | 3:29.33 | Marina Smith (11), Ana Henderson (10), Heidi Poppe (11), Paige Matherson (11) | San Ramon Valley High School | Danville | CA |
| 52 | 3:29.35 | Natalie Feola (11), Leah Smith (12), Alexandra Elizeus (12), Peyton Kondis (9 ) | Oakland Catholic High School | Pittsburgh | PA |
| 53 | 3:29.39 | Isabel Whited (9 ), Kendra Griffin (12), Sam Fazio (10), Victoria Toris (10) | Pinnacle High School | Phoenix | AZ |
| 54 | 3:29.45 | Jordan Wheeler (9 ), Blair Fose (10), Sierra Trambaugh (11), Britney Fant (11) | Atascocita High School | Humble | TX |
| 55 | 3:29.47 | Lindsay Schultz (12), Emily Zapinski (12), Alexa Baran (11), Rebecca Upton (11) | Southlake Carroll High School | Southlake | TX |
| 56 | 3:29.65 | Selby Sturzenegger (11), Erica Myers (12), Kayla Holman (9 ), Ally Howe (11) | Sacred Heart Preparatory | Atherton | CA |
| 57 | 3:29.71 | Cece Williams (11), Alyssa Yambor-Maul (12), Lia Lombardi (12), Delaney Barnard (11) | Lawton Chiles High School | Tallahassee | FL |
| 58 | 3:29.76 | Brianna Daboub (11), Brittany Kampfer (9 ), Jiana Vargem (12), Aislinn Light (9 ) | Mater Dei High School | Santa Ana | CA |
| 58 | 3:29.76 | Riley Schroedter (12), Megan Childs (12), Grace Soltys (11), Gia Dalesandro (12) | Neuqua Valley High School | Naperville | IL |
| 58 | 3:29.76 | Raquel Remick (11), Sydney Franzen (10), Haley Wan (11), Elizabeth Devitt (11) | Santa Margarita Catholic High School | Rancho Santa Margarita | CA |
| 61 | 3:29.78 | Allie Pfauth (12), Jessi Modlich (12), Kelly Sheldon (11), Eryn Eddy (10) | Thompson Valley High School | Loveland | CO |
| 62 | 3:29.87 | Haley Fisher (10), Megan Hawk (11), Morgan Waggoner (9 ), Meghan Moses (9 ) | Fremont Ross High School | Fremont | OH |
| 63 | 3:29.90 | Jennifer Campbell (10), Fiona Hon (11), Nia Gardner (10), Gabrielle Bethke (11) | Gunn High School | Palo Alto | CA |
| 64 | 3:29.98 | Savannah Long (12), Melissa Postoll (12), Maggie Layde (11), Rebecca Postoll (10) | Kennesaw Mountain High School | Kennesaw | GA |
| 65 | 3:30.01 | Shannon Steele (9 ), Casper Cori (11), Kenisha Liu (9 ), Ashley Tse (12) | Ruben S. Ayala High School | Chino Hills | CA |
| 66 | 3:30.02 | Vanessa Krause (9 ), Julia Campbell (12), Kate Curley (12), Kelly Craig (9 ) | Chesterton High School | Chesterton | IN |
| 67 | 3:30.06 | Katie Portz (9 ), Rebecca Dickey (12), Ashleigh Shanley (12), Shannon Cowley (11) | Ann Arbor Skyline High School | Ann Arbor | MI |
| 68 | 3:30.07 | Lindsey Engel (11), Lizzie Saylor (11), Emily Eastin (9 ), Ella Eastin (10) | Crean Lutheran High School | Irvine | CA |
| 68 | 3:30.07 | Erin Falconer (10), Jennifer Kordik (10), Rachel Robb (10), Karrie Kozokar (11) | Mundelein High School | Mundelein | IL |
| 70 | 3:30.09 | Christiana Cornea (10), Jenny Nusbaum (9 ), Hannah Moore (11), Brooke Rubendall (9 ) | Green Hope High School | Cary | NC |
| 71 | 3:30.13 | Kathleen Cook (11), Caitlin Cooper (11), Sarah Coats (11), Joelle Borchers (10) | Woodward Academy | College Park | GA |
| 72 | 3:30.16 | Kimberly Hill (11), Cristina Frias (11), Katie Evans (11), Madeleine Dunn (12) | Greenwich High School | Greenwich | CT |
| 73 | 3:30.26 | Monica Sowinski (10), Madeleine Hoopes (12), Gina DeFrancesco (11), Kayla Owens (11) | Greater Latrobe High School | Latrobe | PA |
| 73 | 3:30.26 | Lexie Lupton (10), Lauren Day (12), Madeline Hazle (12), Katheryn Nolen (12) | Kingwood High School | Kingwood | TX |
| 75 | 3:30.29 | Rebeca Martinez (11), Marysol Arce (10), Natalie Tapia (9 ), Nikol Lagodzinska (11) | St. Ignatius College Prep | Chicago | IL |
| 76 | 3:30.32 | Gabrielle DeLoof (11), Cassandra Morse (11), Jennifer Maiorana (9 ), Catherine DeLoof (10) | Grosse Pointe South High School | Grosse Pointe Farms | MI |
| 77 | 3:30.40 | Anna Petty (9 ), Zoe Tu (11), Kirsten Olson (12), Emma Paulson (12) | Wayzata High School | Plymouth | MN |
| 78 | 3:30.42 | Mary Grace VanAllen (12), Sarah Carlson (11), Shea Hanley (12), Rachel Hayden (11) | North Central High School | Indianapolis | IN |
| 79 | 3:30.45 | Michelle Chintanaphol (12), Kate Beuter (12), Morgan Flynn (12), Elizabeth Stinson (10) | Harpeth Hall School | Nashville | TN |
| 80 | 3:30.48 | Caroline Baldwin (11), Gwyn Devin (9 ), Elinor Reinhardt (12), Sarah Cronin (11) | Westfield High School | Westfield | NJ |
| 81 | 3:30.53 | Kylie Sutherlin (9 ), Allison Barnes (9 ), Robyn Clevenger (9 ), Natalie Mudd (9 ) | Hamilton Southeastern High School | Fishers | IN |
| 81 | 3:30.53 | Anna Babinec (12), Colleen VandePoel (12), Lauren DeShaw (12), Elizabeth Fris (12) | Holland West Ottawa High School | Holland | MI |
| 83 | 3:30.56 | Mary Margaret Soderberg (12), Kristina Domaschofsky (10), Vanessa Duran (12), Sarah Gibson (12) | Ronald Reagan High School | San Antonio | TX |
| 83 | 3:30.56 | Dee Gerke (11), Stormy Malarik (11), Chanel Bonin (11), Rachel Green (12) | South Lyon High School | South Lyon | MI |
| 85 | 3:30.58 | Natalie O'Brien (11), Shannon Cahalan (10), Carly Chelovitz (10), Katelyn Miller (12) | Akron Firestone High School | Akron | OH |
| 85 | 3:30.58 | Siena Salvaggio (9 ), Abbigail Walsh (12), Erika Freeman (10), Megan Polaha (12) | Parkland High School | Allentown | PA |
| 87 | 3:30.64 | Victoria Springman (9 ), Elizabeth Markiewicz (12), Tracilyn Muszalski (11), Holly Spears (11) | Lake Central High School | St. John | IN |
| 88 | 3:30.67 | Maddie Rusch (12), Alex Aliapoulios (11), Meghan Egan (9 ), Jenna Egan (11) | New Canaan High School | New Canaan | CT |
| 89 | 3:30.68 | Mary Beth Blocher (11), Ashley Hwang (11), Allison Cunningham (10), Nicole Smith (10) | Munster High School | Munster | IN |
| 90 | 3:30.76 | Danielle Taylor (10), Hannah Bergh (11), Sarah Wang (9 ), Marissa Bergh (12) | Dougherty Valley High School | San Ramon | CA |
| 90 | 3:30.76 | Kristine Rosenberger (12), Louisa Hoffman (12), Mackenna Dunn (9 ), Elly Vitek (12) | Lyons Township High School | Lagrange | IL |
| 92 | 3:30.84 | Anissa Barroso (9 ), Nicole Item (10), Claire Fisch (9 ), Alex Wolf (11) | Martin Luther King High School | Riverside | CA |
| 93 | 3:30.86 | Jordan Rowe (11), Emily Mayo (12), Anna Hansen (10), Ella Moynihan (10) | Arapahoe High School | Centennial | CO |
| 94 | 3:30.90 | Annie Valentine (12), Maddy Loniewski (10), Kathleen McGee (10), Roxanne Griffore (10) | Mercy High School | Farmington Hills | MI |
| 95 | 3:30.96 | Brittany Kamper (12), Kim Katner (12), Caitlin Krull (11), Clare Lawlor (9 ) | Carl Sandburg High School | Orland Park | IL |
| 96 | 3:31.03 | Samantha Park (12), Lauren Henasey (9 ), Tommie Dillione (11), Lauren Mitchell (10) | Council Rock High School North | Newtown | PA |
| 97 | 3:31.04 | Kiley Eble (10), Agnes Mirando (11), Abby Koerwitz (9 ), Macie McNichols (11) | Gilmour Academy | Gates Mills | OH |
| 98 | 3:31.21 | Angela Algee (12), Emily Kliewer (10), Cindy Cheng (11), Allie Magrino (11) | Dr. Phillips High School | Lando | FL |
| 99 | 3:31.23 | Melissa Yau (11), Madison Fitzgerald (11), Sariyah Sherry (9 ), Emily Schroeder (10) | The Peddie School | Hightstown | NJ |
| 100 | 3:31.31 | Evan Hundley (10), Nicole Oppel (10), Ashton Ehrecke (10), Gabbie Stecker (12) | Pleasant Valley High School | Riverdale | IA |