| Place | Time | Swimmer (Yr) | School | City | State |
| 1 | :51.78 | Kathryn McLaughlin (11) | Santa Margarita Catholic High School | Rancho Santa Margarita | CA |
| 2 | :52.06 | Beata Nelson (10) | Verona / Mount Horeb Co-op | Verona | WI |
| 3 | :52.23 | Jasmine K Mau (12) | Punahou School | Honolulu | HI |
| 4 | :52.46 | Janet Hu (12) | Oakton High School | Vienna | VA |
| 5 | :52.70 | Aly Tetzloff (11) | Crown Point High School | Crown Point | IN |
| 6 | :52.90 | Katie Grover (12) | Milton High School | Milton | GA |
| 7 | :53.41 | Danielle Nack (12) | Mankato West High School | Mankato | MN |
| 8 | :53.45 | Grace Oglesby (10) | North Oldham High School | Goshen | KY |
| 9 | :53.46 | Brittany Usinger (11) | Acalanes High School | Lafayette | CA |
| 10 | :53.62 | Mimi Schneider (12) | Fenwick High School | Oak Park | IL |
| 11 | :53.72 | Alyssa Marsh (10) | Pine Lake Preparatory | Mooresville | NC |
| 12 | :53.74 | Vanessa Krause (10) | Chesterton High School | Chesterton | IN |
| 13 | :53.75 | Tamara Santoyo (11) | William S. Hart High School | Newhall | CA |
| 14 | :53.77 | Breanna Robinson (11) | Wawasee High School | Syracuse | IN |
| 15 | :53.80 | Kylie Jordan (10) | The Madeira School | Mclean | VA |
| 16 | :53.81 | Nicole Smith (11) | Munster High School | Munster | IN |
| 17 | :53.88 | Madison Wright (11) | Waterford United | Waterford | MI |
| 18 | :53.99 | Victoria Edwards (9 ) | Westlake High School | Austin | TX |
| 19 | :54.01 | Taylor Garcia (11) | Holland High School | Holland | MI |
| 20 | :54.03 | Breanna Sapienza (12) | Cascade Christian High School | Medford | OR |
| 21 | :54.06 | Leah Goldman (12) | Burlingame High School | Burlingame | CA |
| 21 | :54.06 | Madelyn Murphy (10) | Carondelet High School | Concord | CA |
| 23 | :54.23 | Paige Maynard (11) | Vacaville High School | Vacaville | CA |
| 23 | :54.23 | Isabel Reis (11) | Berwick Academy | South Berwick | ME |
| 25 | :54.32 | Kenna Ramey (12) | Federal Way High School | Federal Way | WA |
| 26 | :54.33 | Gianna Garcia (10) | San Ramon Valley High School | Danville | CA |
| 27 | :54.34 | Bailey Nero (11) | Fossil Ridge High School | Fort Collins | CO |
| 27 | :54.34 | Anna Quinn (12) | Thousand Oaks High School | Thousand Oaks | CA |
| 29 | :54.37 | Zoe Avestruz (11) | Chanhassen High School | Chanhassen | MN |
| 30 | :54.39 | Aja Malone (12) | Allatoona High School | Acworth | GA |
| 30 | :54.39 | Megan Moroney (11) | Saint Andrew's School | Boca Raton | FL |
| 32 | :54.42 | Heidi Katter (11) | Castilleja School | Palo Alto | CA |
| 33 | :54.43 | Taite Kitchel (11) | Zionsville Community High School | Zionsville | IN |
| 34 | :54.44 | Emily Gibson (12) | Southlake Carroll High School | Southlake | TX |
| 34 | :54.44 | Hanna House (12) | Carmel High School | Carmel | IN |
| 36 | :54.46 | Alex Grimes (11) | Los Gatos High School | Los Gatos | CA |
| 36 | :54.46 | Jennifer Marrkand (12) | Westford Academy | Westford | MA |
| 38 | :54.47 | Heather Merritt (12) | William A. Hough High School | Cornelius | NC |
| 38 | :54.47 | Elsa Welshofer (12) | Charlotte Catholic High School | Charlotte | NC |
| 40 | :54.49 | Lillian Moore (12) | Skyline High School | Salt Lake City | UT |
| 40 | :54.49 | Emma Waddell (12) | Bangor High School | Bangor | ME |
| 42 | :54.52 | Alexis Margett (9 ) | Glendora High School | Glendora | CA |
| 43 | :54.56 | Lauren Case (10) | Woodsotck High School | Woodstock | GA |
| 44 | :54.61 | Niki Price (12) | Northeastern High School | Manchester | PA |
| 45 | :54.63 | Emily Eastin (10) | Crean Lutheran High School | Irvine | CA |
| 46 | :54.65 | Summer Campbell (12) | Paschal High School | Fort Worth | TX |
| 47 | :54.66 | Christine Jensen (11) | Castle High School | Newburgh | IN |
| 48 | :54.68 | Megan Doty (10) | Arrowhead High School | Hartland | WI |
| 49 | :54.71 | Laura Branton (11) | Oakton High School | Vienna | VA |
| 49 | :54.71 | Kelly McNamara (11) | Addison Trail/Willowbrook Co-op | Addison | IL |
| 51 | :54.73 | Maddy Burnham (12) | Bettendorf High School | Bettendorf | IA |
| 52 | :54.77 | Claire DeSelm (10) | Chapel Hill High School | Chapel Hill | NC |
| 53 | :54.78 | Emily Rhodes (12) | Episcopal Academy | Newtown Square | PA |
| 53 | :54.78 | Caitlin Tycz (9 ) | Brunswick High School | Brunswick | ME |
| 55 | :54.80 | Anna Brolin (12) | Gilroy High School | Gilroy | CA |
| 55 | :54.80 | Raena Eldridge (11) | Rockwall High School | Rockwall | TX |
| 57 | :54.86 | Kayla Churman (11) | North Allegheny Senior High School | Pittsburgh | PA |
| 58 | :54.87 | Kara Eisenmann (9 ) | Lewisville High School | Lewisville | TX |
| 58 | :54.87 | Kierston Farley-Sepe (10) | Waukesha South High School | Waukesha | WI |
| 60 | :54.89 | Hannah Musser (12) | Blue Valley Northwest High School | Overland Park | KS |
| 61 | :54.91 | Rebecca Luft (10) | Northern York High School | Dillsburg | PA |
| 62 | :54.93 | Valerie Yoshimura (11) | Baldwin School | Bryn Mawr | PA |
| 63 | :54.96 | Meaghan Raab (12) | Hershey High School | Hershey | PA |
| 64 | :54.97 | Colby Harvey (12) | Waynflete School | Portland | ME |
| 65 | :54.99 | Grace Dimeo (12) | Booker High School | Sarasota | FL |
| 66 | :55.01 | Leah Rogers (11) | W. T. Woodson High School | Fairfax | VA |
| 67 | :55.03 | Maddy Sims (12) | Downers Grove North High School | Downers Grove | IL |
| 68 | :55.04 | Chanel Bonin (12) | South Lyon High School | South Lyon | MI |
| 69 | :55.06 | Chloe Isleta (10) | Presentation High School | San Jose | CA |
| 70 | :55.07 | Megan Bestor (12) | Lambert High School | Suwanee | GA |
| 71 | :55.11 | Hannah James (12) | Charlotte High School | Punta Gorda | FL |
| 72 | :55.12 | Elise Hart (12) | Cartersville High School | Cartersville | GA |
| 73 | :55.16 | Julia Box (11) | Fairview High School | Boulder | CO |
| 73 | :55.16 | Kiley Eble (11) | Gilmour Academy | Gates Mills | OH |
| 75 | :55.23 | Ashley Johnson (11) | Langham Creek High School | Houston | TX |
| 76 | :55.24 | Madison Myers (11) | Copley High School | Copley | OH |
| 77 | :55.25 | Courtney Harnish (9 ) | West York Area High School | York | PA |
| 78 | :55.27 | Reilly Lanigan (10) | Lake Forest High School | Lake Forest | IL |
| 78 | :55.27 | Sara Metzsch (10) | Century High School | Hillsboro | OR |
| 80 | :55.28 | Heaven Quintana (10) | Granite Bay High School | Granite Bay | CA |
| 81 | :55.32 | Dana Posthuma (11) | Omaha Burke High School | Omaha | NE |
| 82 | :55.35 | Kylie Cronin (10) | Marblehead High School | Marblehead | MA |
| 82 | :55.35 | Natasha Gvakharia Mgebrishvili (11) | The Bolles School | Jacksonville | FL |
| 82 | :55.35 | Amanda McNulty (12) | J.L. Mann High School | Greenville | SC |
| 85 | :55.36 | Julieyanna Parker (10) | Hillsborough High School | Hillsborough | NJ |
| 86 | :55.37 | Madison Hart (10) | Haverford High School | Havertown | PA |
| 86 | :55.37 | Paige Madden (9 ) | UMS-Wright Preparatory School | Mobile | AL |
| 88 | :55.38 | Amelia Bullock (10) | Staples High School | Westport | CT |
| 89 | :55.39 | Morgan Bullock (10) | Zeeland High School | Zeeland | MI |
| 89 | :55.39 | Kayla Tennant (9 ) | Gulf Coast High School | Naples | FL |
| 91 | :55.41 | Haley Bishop (11) | Panther Creek High School | Cary | NC |
| 91 | :55.41 | Remedy Rule (11) | Western Albemarle High School | Crozet | VA |
| 91 | :55.41 | Astrid Swensen (11) | Beverly High School | Beverly | MA |
| 94 | :55.42 | Abigail Miller (10) | San Ramon Valley High School | Danville | CA |
| 95 | :55.44 | Nancy Hu (11) | Riverview High School | Sarasota | FL |
| 96 | :55.47 | Mikayla Ranslem (11) | William S. Hart High School | Newhall | CA |
| 97 | :55.48 | Julianna Foss (12) | Shawnee High School | Medford | NJ |
| 97 | :55.48 | Ariana Saghafi (11) | Albany High School | Albany | CA |
| 97 | :55.48 | Breinne Thorne (10) | Eden Prairie High School | Eden Prairie | MN |
| 100 | :55.54 | Robyn Clevenger (10) | Hamilton Southeastern High School | Fishers | IN |