| Place | Time | Relay Swimmers (Yrs) | School | City | State |
| 1 | 1:31.37 | Amy Bilquist (11), Claire Adams (10), Hanna House (12), Veronica Burchill (10) | Carmel High School | Carmel | IN |
| 2 | 1:32.74 | Kasey Schmidt (11), Claire Rasmus (11), Natasha Gvakharia Mgebrishvili (11), Jessica Hodgson (12) | The Bolles School | Jacksonville | FL |
| 3 | 1:33.22 | Rebecca Postoll (11), Maria Sheridan (12), Lauren Rhodes (12), Nora McCullagh (11) | Charlotte Catholic High School | Charlotte | NC |
| 4 | 1:33.51 | Heidi Poppe (12), Marina Smith (12), Alexa Fusari (9 ), Cailey Steffens (11) | San Ramon Valley High School | Danville | CA |
| 5 | 1:33.62 | Madelyn Murphy (10), Kelsey Witkay (10), Malorie Han (10), Natalie Amberg (12) | Carondelet High School | Concord | CA |
| 6 | 1:33.70 | Maizie Seidl (9 ), Kristi Larsen (9 ), Shelby Rozeboom (11), Beata Nelson (10) | Verona / Mount Horeb Co-op | Verona | WI |
| 7 | 1:33.81 | Kaley Buchanan (9 ), Jenny Young (12), Colette Robinson (10), Madelyn Veith (12) | Hershey High School | Hershey | PA |
| 8 | 1:33.87 | GraceAnne Long (11), Alexandra Hedden (11), Grace Van Fossen (11), Jennifer Smith (12) | Upper Arlington High School | Upper Arlington | OH |
| 9 | 1:34.06 | Amelia Anderson (12), Olivia Anderson (11), Emma Wittmer (11), Rachel Wittmer (9 ) | Edina High School | Edina | MN |
| 10 | 1:34.12 | Kelly Jacob (10), Maddie Barta (11), Samantha Kultgen (12), Mary Warren (12) | Arrowhead High School | Hartland | WI |
| 11 | 1:34.19 | Ella Eastin (11), Emily Eastin (10), Tianna Jorgenson (10), Lindsey Engel (12) | Crean Lutheran High School | Irvine | CA |
| 12 | 1:34.21 | Bethany Galat (12), Annie Spalding (12), Casey Ziegert (11), Emily Meckstroth (11) | Penn High School | Mishawaka | IN |
| 13 | 1:34.35 | Colleen Donlin (10), Sarah Lingen (10), Megan Larson (11), Madison Preiss (10) | Wayzata High School | Plymouth | MN |
| 14 | 1:34.49 | Abby Jagdfeld (12), Molly Manchon (11), Kaersten Meitz (12), Kierston Farley-Sepe (10) | Waukesha South High School | Waukesha | WI |
| 15 | 1:34.58 | Erin Falconer (11), Jennifer Kordik (11), Rachel Robb (11), Karrie Kozokar (12) | Mundelein High School | Mundelein | IL |
| 16 | 1:34.64 | Camryn Barry (9 ), Raquel Remick (12), Elizabeth Devitt (12), Kathryn McLaughlin (11) | Santa Margarita Catholic High School | Rancho Santa Margarita | CA |
| 17 | 1:34.78 | Kate Herrill (10), Katie Altmayer (12), Christina Campbell (9 ), Kirsten Vose (11) | Flintridge Sacred Heart Academy | La Canada Flintridge | CA |
| 18 | 1:34.81 | Ashley Volpenhein (9 ), Abbey Esler (10), Julia Volpenhein (11), Maureen Sullivan (12) | Mason High School | Mason | OH |
| 19 | 1:34.87 | Caitlin Cooper (12), Joelle Borchers (11), Charlotte Bernot (10), Kathleen Cook (12) | Woodward Academy | College Park | GA |
| 20 | 1:34.93 | Sarah Kaunitz (12), Christina Cheng (11), Lisa Kaunitz (10), Carly Reid (12) | Monta Vista High School | Cupertino | CA |
| 21 | 1:34.96 | Brenna Ruth (12), McKenna Keith (11), CJ McCartin (10), Megan Moroney (11) | Saint Andrew's School | Boca Raton | FL |
| 22 | 1:35.02 | Molly O'Hara (10), Ashley LeClair (12), Shea Maloney (10), Leah Stevens (11) | Sacred Heart Academy | Louisville | KY |
| 23 | 1:35.12 | Shannon McKernan (11), Meagan Dollard (12), Madison Visco (11), Natalie Dowzicky (12) | Germantown Academy | Fort Washington | PA |
| 24 | 1:35.24 | Maren Taylor (10), Hannah Richard (11), Sophia Schott (9 ), Mollie Pulte (11) | Marian High School | Bloomfield Hills | MI |
| 25 | 1:35.28 | Morgan Soulia (11), Lauren Mcgrath (9 ), Maggie Root (11), Jade Song (12) | North Allegheny Senior High School | Wexford | PA |
| 26 | 1:35.31 | Roxy Griffore (11), Maddy Loniewski (11), Alaina Skellett (9 ), Kathleen McGee (11) | Mercy High School | Farmington Hills | MI |
| 27 | 1:35.34 | Stephanie Andrews (12), Emma Corby (9 ), Lindsay Stone (9 ), Katie Smith (10) | Pittsford Central Schools | Pittsford | NY |
| 28 | 1:35.37 | Emily Albrecht (11), Daria Wick (11), Lindsay Mathys (9 ), Maddy Sims (12) | Downers Grove North High School | Downers Grove | IL |
| 29 | 1:35.44 | Mary Beth Blocher (12), Megan Wimmer (9 ), Allison Cunningham (11), Nicole Smith (11) | Munster High School | Munster | IN |
| 30 | 1:35.61 | Sydney Higgins (10), Kathleen Feller (11), Mary First (10), Johanna Jekel (12) | Madison West High School | Madison | WI |
| 31 | 1:35.64 | Morgan Waggoner (10), Megan Hawk (12), Haley Fisher (11), Meghan Moses (10) | Fremont Ross High School | Fremont | OH |
| 32 | 1:35.65 | Kiley Eble (11), Agnes Mirando (12), Abby Koerwitz (10), Macie McNichols (12) | Gilmour Academy | Gates Mills | OH |
| 32 | 1:35.65 | Robyn Clevenger (10), Natalie Mudd (10), Lauren Blanchard (10), Allie Barnes (10) | Hamilton Southeastern High School | Fishers | IN |
| 34 | 1:35.70 | Anna Justinak (10), Sophie Levy (11), Abigail Saddler (12), Isabel Wyer (12) | Minnetonka High School | Minnetonka | MN |
| 35 | 1:35.73 | Olivia Calegan (9 ), Dannie Dilsaver (9 ), Emma Vertin (10), Aimee Fischer (12) | Lincoln Southwest High School | Lincoln | NE |
| 36 | 1:35.75 | Vanessa Krause (10), Cara Kroeger (9 ), Amy Socha (9 ), Kelly Craig (10) | Chesterton High School | Chesterton | IN |
| 37 | 1:35.83 | Lauren Ramirez (11), Hannah Crane (12), Shannon Carney (10), Maddy Burnham (12) | Bettendorf High School | Bettendorf | IA |
| 38 | 1:35.88 | Courtney Kresl (10), Abigail Johnston (9 ), Mykenzie Kostka (10), Zoe Toscos (10) | Carroll High School | Fort Wayne | IN |
| 38 | 1:35.88 | Summer Spradley (11), Julienne Tadena (11), Kassidy Henson (10), Jordan Anderson (11) | Granite Bay High School | Granite Bay | CA |
| 40 | 1:35.89 | Julieyanna Parker (10), Deborah Stoddard (11), Megan Bull (9 ), Lindsay Temple (12) | Hillsborough High School | Hillsborough | NJ |
| 41 | 1:35.91 | Aley Shimel (9 ), Kelsey Opiela (11), Emily Bocock (9 ), Lexie Lupton (11) | Kingwood High School | Kingwood | TX |
| 41 | 1:35.91 | Megan Bestor (12), Emma Fish (10), Aubrey Jones (12), Katherine Aikins (10) | Lambert High School | Suwanee | GA |
| 43 | 1:35.95 | Bailey Moynihan (12), Katie Wells (11), Diana Capota (11), Kelly Cordes (11) | Glenbrook South High School | Glenview | IL |
| 43 | 1:35.95 | Kaitlyn Albertoli (11), Brittany Kahn (11), Katie Glavinovich (9 ), Karli Thuen (11) | Jserra Catholic High School | San Juan Capistrano | CA |
| 45 | 1:35.98 | Melissa Karman (11), Maggie Stovall (11), Leslie Campbell (11), Breanna Roman (12) | The Baylor School | Chattanooga | TN |
| 46 | 1:35.99 | Marta Ciesla (9 ), Julia Chui (10), Brooke Bernstein (9 ), Hannah Veale (12) | Pine Crest School | Fort Lauderdale | FL |
| 47 | 1:36.00 | Laura Norman (12), Sophia Frohberg (10), Audrey McMurry (9 ), Megan Hanson (9 ) | Keller High School | Keller | TX |
| 47 | 1:36.00 | Brittany Bishop (11), Grace Ford (12), Nicole Retondo (11), Amelia Girgenti (12) | New Trier High School | Winnetka | IL |
| 49 | 1:36.05 | Lexus VanHoven (9 ), Hanna Sanford (9 ), Emliy Converse (10), Gabby Higgins (9 ) | East Grand Rapids High School | Grand Rapids | MI |
| 49 | 1:36.05 | Zoe Rosin (10), Isabel Whited (10), Samantha Fazio (11), Victoria Toris (11) | Pinnacle High School | Phoenix | AZ |
| 51 | 1:36.09 | Lauren Betancourt (11), Alecia Camp (11), Kaitlyn Hunt (9 ), Leah Sims (12) | Oviedo High School | Oviedo School | FL |
| 52 | 1:36.10 | Daria Pyshnenko (9 ), Eleanor Meeks (11), Madeline Smith (9 ), Reilly Lanigan (10) | Lake Forest High School | Lake Forest | IL |
| 53 | 1:36.14 | Wheeler Jordan (10), Petersen Bethany (9 ), Wehrmeister Sarah (10), Fant Britney (12) | Atascocita High School | Humble | TX |
| 53 | 1:36.14 | Lily Gasaway (12), Kelleigh Haley (10), Laura Garcia (12), Katie Ledecky (11) | Stone Ridge School | Bethesda | MD |
| 55 | 1:36.19 | Racheal Bukowski (12), Kelsi Artim (9 ), Mackenzie Artim (10), Holly Schuster (12) | Crown Point High School | Crown Point | IN |
| 56 | 1:36.20 | Alexis Lemone (10), Sydney Mathis (9 ), Isabelle Jubin (11), Caroline McTaggart (11) | The Holton Arms School | Bethesda | MD |
| 57 | 1:36.22 | Leah Brown (10), Tara Rogers (12), Aimee Baur (10), Olivia DiStefano (11) | North Penn High School | Lansdale | PA |
| 58 | 1:36.23 | Elise Bourdelais (10), Julia Capobianco (11), Megan Byrnes (10), Laura Branton (11) | Oakton High School | Vienna | VA |
| 59 | 1:36.30 | Colleen Barron (12), Adelaide Curry (12), Theresa Mullen (10), Maggie Sheean (12) | Fenwick High School | Oak Park | IL |
| 60 | 1:36.33 | Kathryn Nicholson (9 ), Katie Ford (12), Meghan Schilken (12), Veronica Bogdanski (11) | Mt. Lebanon High School | Pittsburgh | PA |
| 60 | 1:36.33 | Vanessa Ruiz (10), Nika Blank (10), Madison Fitzgerald (12), Emily Schroeder (11) | The Peddie School | Hightstown | NJ |
| 62 | 1:36.34 | Bryanna Hundt (12), Mina Glenesk (12), Maggie Taylor (9 ), Catriona Macgregor (9 ) | Westlake High School | Austin | TX |
| 63 | 1:36.38 | Caroline Baldwin (12), Gwyn Devin (10), Allison Mcbrearty (12), Sarah Cronin (12) | Westfield High School | Westfield | NJ |
| 64 | 1:36.39 | Lauren Cuda (12), Elizabeth Ryan (10), Ellery Parish (9 ), Anelise Diener (11) | Alamo Heights High School | San Antonio, Texas | TX |
| 65 | 1:36.41 | Emily Cornell (10), Maura Mcmanus (9 ), Olivia Giampietro (9 ), Julie Dobson (11) | Villa Maria Academy | Malvern | PA |
| 66 | 1:36.43 | Hope Christy (9 ), Kathleen Hanley (9 ), Reagan Roob (9 ), Rachel Hayden (12) | North Central High School | Indianapolis | IN |
| 67 | 1:36.46 | Kayla Owens (12), Gina DeFrancesco (12), Megan Douds (10), Monica Sowinski (11) | Greater Latrobe High School | Latrobe | PA |
| 68 | 1:36.52 | Ally Howe (12), Kayla Holman (10), Kathryn Bower (9 ), Selby Sturzenegger (12) | Sacred Heart Preparatory | Atherton | CA |
| 69 | 1:36.60 | Rachel Thompson (12), Brooke Maxson (10), Kathleen Hungerford (11), Emily Konishi (11) | Foothill High School | Santa Ana | CA |
| 69 | 1:36.60 | Claire Rushin (11), Olivia Andrew (9 ), Maria Kyle (10), Grace Tierney (11) | Loyola Academy | Wilmette | IL |
| 69 | 1:36.60 | Natalie Tapia (10), Clare Macnamara (10), Rebeca Martinez (12), Nikol Lagodzinska (12) | St. Ignatius College Prep | Chicago | IL |
| 72 | 1:36.63 | Lauren Williams (12), Georgia Brown (12), Carly Straight (10), Kayla Epting (11) | Keller Central High School | Keller | TX |
| 72 | 1:36.63 | Erin Hart (11), Alexis Yager (9 ), Georgia Young (10), Elaina Ricci (11) | Rosary High School | Aurora | IL |
| 74 | 1:36.66 | Sarah Medellin (12), Tori Kroon (12), Erin Sutton (11), Kathryn Carson (12) | Cinco Ranch High School | Katy | TX |
| 75 | 1:36.67 | Brooke Delventhal (11), Gabi D'Abato (12), Julia Grady (11), Molly Washko (12) | Strongsville High School | Strongsville | OH |
| 76 | 1:36.71 | Marissa Marion (11), Dakota Noble (11), Madison Trimble (12), Erin Hudson (11) | Rockford High School | Rockford | MI |
| 77 | 1:36.73 | Katie Krivacs (12), Megan Johnson (9 ), Jordan Nussear (10), Emily Thrasher (10) | Homestead High School | Fort Wayne | IN |
| 78 | 1:36.74 | Alexa Steffl (11), Madeline Hunt (12), Emma Tinney (9 ), Avery Mathew (10) | Waubonsie Valley High School | Aurora | IL |
| 79 | 1:36.76 | Emma Broome (9 ), Olivia Wang (11), Gabriella Fagan (9 ), Emma Segneri (9 ) | Chaparral High School | Scottsdale | AZ |
| 80 | 1:36.79 | Spence Atkins (11), Laura Toth (12), Niamh O'Grady (12), Rachel O'Grady (12) | Riverview High School | Sarasota | FL |
| 81 | 1:36.83 | Miranda Donley (9 ), Eleanor Smith (10), Erin Balasky (12), Rachel Politi (11) | Columbus School For Girls | Columbus | OH |
| 81 | 1:36.83 | Madeline Salesky (11), Olivia Lewis (10), Nuoshi Lu (9 ), Isabelle Kitze (12) | Marin Academy | San Rafael | CA |
| 83 | 1:36.86 | Elise Goetzl (12), Marie Claire Schillinger (11), Sofia Marinac (12), Kristen Hong (11) | Miramonte High School | Orinda | CA |
| 84 | 1:36.88 | Sara Metzsch (10), Hannah Galbraith (10), Logan Neal (11), Thornbrue Ellie (11) | Century High School | Hillsboro | OR |
| 84 | 1:36.88 | Chantal Nack (11), Madison Bacon (11), Rachel Phinney (9 ), Danielle Nack (12) | Mankato West High School | Mankato | MN |
| 86 | 1:36.89 | Samantha Giannetta (11), Sarah Snyder (10), Skylar Elkington (9 ), Mary Brooks (12) | Clovis West High School | Fresno | CA |
| 87 | 1:36.92 | Tamara Santoyo (11), Alyssa Schultz (10), Emma Osowski (12), Ranslem Mikayla (11) | William S. Hart High School | Newhall | CA |
| 88 | 1:36.94 | Joyce Lin (9 ), Marie-Pierre Delisle (12), Julia Read (10), Sarah Shimomura (11) | Archbishop Mitty High School | San Jose | CA |
| 89 | 1:36.96 | Gabrielle Bethke (12), Fiona Hon (12), Sam Acker (11), Caroline Anderson (12) | Gunn High School | Palo Alto | CA |
| 89 | 1:36.96 | Amy Lenderink (10), Isabella Schultze (9 ), Samantha Smith (9 ), Taylor Wilson (11) | Regis Jesuit High School | Aurora | CO |
| 91 | 1:36.98 | Jenna Egan (12), Libby O'Hare (10), Alex Aliapoulios (12), Meghan Egan (10) | New Canaan High School | New Canaan | CT |
| 92 | 1:37.01 | Lilia Staszel (10), Dominique Meldrum (11), Danika Meldrum (11), Mckenna Resconich (10) | Brighton High School | Brighton | MI |
| 92 | 1:37.01 | Sarah Rasmussen (12), Mary Ashby (11), Brooke Presten (11), Emily Ward (10) | Campolindo High School | Moraga | CA |
| 94 | 1:37.04 | Sienna Stonesmith (11), Jessie Li (11), Logan Tidstrom (10), Brittney Beetcher (9 ) | Fairview High School | Boulder | CO |
| 94 | 1:37.04 | Amber Kovach (11), Heather Svitavsky (10), Beth Fitzgerald (12), Crile Hart (9 ) | Hawken School | Gates Mills | OH |
| 94 | 1:37.04 | Amelia Armstrong-Grant (10), Lisa Gross (9 ), Alex Mcpherson (11), Serena Yan (12) | Saline Area Schools | Saline | MI |
| 97 | 1:37.05 | Olivia Kirkpatrick (10), Alex Nuyianes (11), Konner Brewer (11), Clare Fleming (12) | Academy Of The Holy Names | Tampa | FL |
| 98 | 1:37.07 | Lauren O'Malley (11), Alejandra Ramirez (11), Anna Lofton (9 ), Alexis Glunn (10) | Lassiter High School | Marietta | GA |
| 99 | 1:37.08 | Emma Curtis (12), Emma King (11), Maddison Eckley (10), Nora Fullenkamp (9 ) | Centerville High School | Centerville | OH |
| 100 | 1:37.11 | Laurel Eiber (9 ), Rachel Getz (12), Jessica Brookes (12), Ella Moynihan (11) | Arapahoe High School | Centennial | CO |
| 100 | 1:37.11 | Sammy Sauer (10), Loriel Hutchinson (12), Grace Samuelson (10), Monica Guyett (10) | St. Charles North High School | St. Charles | IL |