| Place | Time | Relay | School | City | State |
| 1 | 1:30.17 | Preston Jenkins (12), Matteus Martini (12), Joseph Schooling (10), Ryan Murphy (11) | The Bolles School | Jacksonville | FL |
| 2 | 1:33.70 | Ryan McRae (11), David Morgan (12), Zachary Perrotti (12), Jason Williams (12) | Palm Harbor University High School | Palm Harbor | FL |
| 3 | 1:33.75 | Payton Sorenson (12), Derek Marske (12), Edgar Febres (11), Derek Buckley (12) | Mountain View High School | Mesa | AZ |
| 4 | 1:35.37 | Dionisio Carey (9 ), Quinn Cassidy (11), Shawn Warner (11), Wali Toulson (12) | Saint Andrew's School | Boca Raton | FL |
| 5 | 1:35.46 | Michael Nelson (12), Timothy Frakes (12), Connor Shores (12), Theodore Decker (11) | Brophy College Preparatory | Phoenix | AZ |
| 6 | 1:35.48 | Marcel Betschart (12), Michal Rokita (12), Adam Dear (11), Descartes Holland (11) | Pine Crest School | Fort Lauderdale | FL |
| 7 | 1:35.90 | Grant Grenfell (12), Colin Finnegan (12), Chris Cazenave (12), Alec Vicknair (12) | Catholic High School | Baton Rouge | LA |
| 8 | 1:36.59 | Jack Snow (12), Chris Stein (10), Will Brand (10), Luke Snow (12) | Glendale High School | Springfield | MO |