| Place | Time | Swimmers (Yrs) | School | City | State |
| 1 | 3:21.96 | * Hillary Thomas (12), Alex Todd (12), McKenna DeBever (9 ), Missy Franklin (10) | Regis Jesuit High School | Aurora | CO |
| * Note - Regis Jesuit's 3:21.96 was altituded adjusted - Official unadjusted time is 3:22.42 | |||||
| 2 | 3:22.85 | Samantha Surfus (10), Meaghan Raab (9 ), Yurie Nakano (12), Katelyn Miller (12) | Hershey High School | Hershey | PA |
| 3 | 3:23.93 | Bonnie Brandon (12), Mckenna Newsum-Schoenbe (12), Fiona Kane (11), Jordan Mattern (12) | Cherry Creek High School | Greenwood Village | CO |
| 4 | 3:25.00 | Rhiannon Sheets (12), Lauren Jordan (12), Alexis Bullard (11), Margaret Ramsey (11) | Carmel High School | Carmel | IN |
| 5 | 3:25.61 | Lauren Driscoll (11), Jessica Randolph (10), Chantal Bouthillier (11), Lindsey McKnight (11) | American Heritage School | Plantation | FL |
| 6 | 3:26.00 | Olivia Evans (12), Maggie Hofstadter (11), Megan Rankin (11), Cheyenne Low (10) | University High School | Irvine | CA |
| 7 | 3:26.04 | Stephanie Marchuk (10), Megan Marchuk (12), Morgan Scott (11), Samantha James (11) | New Trier High School | Winnetka | IL |
| 8 | 3:26.24 | Rachael Acker (11), Julia Ama (11), Christine Prior (11), Casey Lincoln (11) | Gunn High School | Palo Alto | CA |
| 9 | 3:26.27 | Ellen Williamson (12), Molly Hinken (10), Caitlyn Forman (11), Mackenzie Margroum (10) | Notre Dame Academy | Park Hills | KY |
| 10 | 3:26.32 | Alexis Walley (12), Erica Reifinger (11), Kayla Grim (12), Emily Stang (11) | Parkland High School | Allentown | PA |
| 11 | 3:26.42 | Meagan Dollard (9 ), Angie Sawick (12), Bridget Casey (12), Rachel Zilinskas (10) | Germantown Academy | Fort Washington | PA |
| 12 | 3:26.47 | Natalie Amberg (9 ), Nicole Palmer (11), Madison White (10), Chelsea Chenault (10) | Carondelet High School | Concord | CA |
| 13 | 3:26.71 | Gabrielle Veri (11), Kayla Hammerberg (12), Lauren Comer (11), Abigail Chin (12) | Upper Arlington High School | Upper Arlington | OH |
| 14 | 3:26.96 | Lauren Neidigh (12), Stacey Stanfield (11), Marina Blum (11), Megan Fonteno (12) | The Bolles School | Jacksonville | FL |
| 15 | 3:27.07 | Maddy Schaefer (12), Jessie Ho (10), Joyce Zhou (10), Jacqui Levere (12) | Saint Francis High School | Mountain View | CA |
| 16 | 3:27.18 | Jillian Vitarius (11), Shannen Wehrman (12), Madeline Hazle (10), Jamie Friderichs (11) | Kingwood High School | Kingwood | TX |
| 17 | 3:27.24 | Olivia Samoray (11), Sato Kakihara (11), Maria Bargardi (12), Nikki Barczak (12) | Mercy High School | Farmington Hills | MI |
| 18 | 3:27.71 | Ashlee Korsberg (10), Monica Patterson (12), Margaret Dinnocenzo (10), Rachel Moore (11) | Andover High School | Andover | MA |
| 19 | 3:27.72 | Kimberlee John-Williams (10), Hannah Peiffer (10), Bria Deveaux (11), Emma Micahels (12) | The Baylor School | Chattanooga | TN |
| 20 | 3:27.80 | Megan Cox (11), Carly Johnson (11), Jourdan Broadfoot (12), Leticia Lelli (11) | Chaparral High School | Scottsdale | AZ |
| 21 | 3:27.99 | Sabrina Lee (12), Jayna Wittenbrink (11), Molly Zebker (10), Jasmine Tosky (11) | Palo Alto High School | Palo Alto | CA |
| 22 | 3:28.09 | Alexa Morris (12), Sarah Gibson (10), Coralyn Gary (12), Colleen Konetzke (11) | Ronald Reagan High School | San Antonio | TX |
| 23 | 3:28.13 | Caitlin Hanahoe (12), Katherine Bootsma (12), Kira Zubar (9 ), Rachel Bootsma (11) | Eden Prairie High School | Eden Prairie | MN |
| 24 | 3:28.35 | Katie Adams (12), Leah Ortiz (11), Karoline Eckhart (10), Kathryn Eckhart (11) | Cy Creek High School | Houston | TX |
| 25 | 3:28.56 | Marissa La Freniere (10), Rachel Johnson (11), Tara Halsted (10), Beverly Nguyen (11) | Davis Senior High School | Davis | CA |
| 25 | 3:28.56 | Sarah Koucheki (10), Jordan Bitterman (11), Mackenzie Harris (11), Morgan Cohara (11) | Hawken School | Gates Mills | OH |
| 27 | 3:28.63 | Sarah Graif (12), Alisha Bender (11), Talie Anchustegui (11), Kayra Zsiga (12) | Xavier College Preparatory | Phoenix | AZ |
| 28 | 3:28.65 | Aja Van Hout (12), Maddie Martin (9 ), Chelsea Soderholm (11), Ivy Martin (12) | Madison East High School | Madison | WI |
| 29 | 3:28.67 | Kim Carducci (12), Shannon O'Malley (12), Sarah Williford (11), Samantha Bosma (12) | Lassiter High School | Marietta | GA |
| 30 | 3:28.70 | Dani Barbiea (12), Ashley Whiteley (10), Jennifer Garzinski (12), Kellie Barbiea (12) | Springboro High School | Springboro | OH |
| 31 | 3:28.78 | Alli Gillespie (12), Nicole Boyce (12), Alys Williams (11), Ellie Morrissey (11) | Edison High School | Huntington Beach | CA |
| 32 | 3:28.87 | Elizabeth House (10), Konnor Rist (11), Sarah Love (10), Caroline Thomas (12) | DuPont Manual High School | Louisville | KY |
| 33 | 3:28.99 | Evan Swenson (10), Maddie Jardeleza (10), Madeleine Klichowski (12), Mallory Remick (11) | Loyola Academy | Wilmette | IL |
| 34 | 3:29.01 | Haley Pietila (11), Kate Jones (11), Kristen Barta (12), Holly Johnson (12) | Arrowhead Union High School | Hartland | WI |
| 35 | 3:29.05 | Deanna Marks (12), Melissa Leonhardt (11), Shirley Wang (12), Marissa Konicke (12) | Plano Senior High School | Plano | TX |
| 36 | 3:29.09 | Kate Dillione (12), Bizzy Millen (12), Tommie Dillione (9 ), Madison Meyer (11) | Council Rock North High School | Newton | PA |
| 37 | 3:29.15 | Victoria Kuhn (11), Reia Tong (11), Audrey Gould (10), Charlotte Meyer (10) | Walt Whitman High School | Bethesda | MD |
| 38 | 3:29.22 | Allison Brown (11), Paige Matherson (9 ), Taylor Nanfria (12), Marissa Neel (11) | San Ramon Valley High School | Danville | CA |
| 39 | 3:29.23 | Sarah Henry (10), Jordyn Barham (9 ), Hayley Tomlinson (11), Jessica Godlewski (12) | Apex High School | Apex | NC |
| 40 | 3:29.36 | Claire Liu (10), Kristin Connors (11), Carly Catella (9 ), Henriette Stenkvist (11) | Panther Creek High School | Cary | NC |
| 41 | 3:29.45 | Devin Madsen (11), Taylor Carlson (12), Danielle Johnson (11), Kelsey Leonard (10) | Las Lomas High School | Walnut Creek | CA |
| 42 | 3:29.56 | Emilee Duquette (11), Hanna House (9 ), Sarah Lohman (11), Mariah Hamil (11) | Homestead High School | Fort Wayne | IN |
| 43 | 3:29.60 | Katerina Boisov (10), Allie Pennetti (11), Dana Scherer (11), Kally Vanderbilt (11) | North Allegheny Senior High School | Wexford | PA |
| 43 | 3:29.60 | Cassidy Lapp (12), Kaitlyn Hogan (12), Jillian Roberts (12), Emily Zapinski (10) | Southlake Carroll High School | Southlake | TX |
| 45 | 3:29.77 | Haley Sims (12), Jennifer Gockman (10), Maddy Sims (9 ), Gabby Sims (9 ) | Downers Grove North High School | Downers Grove | IL |
| 46 | 3:29.87 | Lindsay Morrow (10), Lauren Sutton (12), Stefania Rossi (12), Jackie Hodges (12) | Cinco Ranch High School | Katy | TX |
| 47 | 3:29.92 | Sydney Johansen (12), Natalie Green (10), Carissa Metcalf (12), Annclaire Macart (9 ) | St. Francis High School | Sacramento | CA |
| 48 | 3:29.98 | Camilla Czulada (11), Elizabeth Ebert (10), Erin Timochenko (11), Caitlyn Karr (12) | Wilson High School | West Lawn | PA |
| 49 | 3:30.00 | Elizabeth Gelb (12), Annie Tamblyn (11), Samadhi Kiridena (9 ), Anna DeMonte (11) | Ann Arbor Huron High School | Ann Arbor | MI |
| 50 | 3:30.01 | Kate Rogers (9 ), Maddy Frost (10), Jordan Rucinski (12), Stephanie Boyle (12) | Saline High School | Saline | MI |
| 51 | 3:30.04 | Kayla Skenandore (11), Abby Jagdfeld (9 ), Kaersten Meitz (9 ), Courtney Sheehan (12) | Waukesha South/Mukwonago High School | Waukesha | WI |
| 52 | 3:30.06 | Mary Schneider (9 ), Cara Borkovec (9 ), Paulina Kaminski (9 ), Maureen Barron (11) | Fenwick High School | Oak Park | IL |
| 53 | 3:30.07 | Meghan Bicomong (10), Courtney Whyte (12), Padon Sivesind (12), Dana Holt (12) | Campolindo High School | Moraga | CA |
| 54 | 3:30.16 | Rachel Astles (11), Katherine Kerman (11), Erika Redding (10), Nicole Stafford (10) | Westminster Schools | Atlanta | GA |
| 55 | 3:30.30 | Ginny Grams (12), Emily Grams (12), Shaun Casey (12), Kendal Casey (11) | Spruce Creek High School | Port Orange | FL |
| 56 | 3:30.32 | Eva Chen (12), Haley Oosterhouse (11), Charlotte Ruby (11), Marie-Pierre Delisle (9 ) | Archbishop Mitty High School | San Jose | CA |
| 57 | 3:30.45 | Holly Morren (9 ), Klare Northuis (11), Haley Thompson (11), Courtney Bartholomew (11) | Holland High School | Holland | MI |
| 58 | 3:30.51 | Rachel Burke (11), Molly Coonce (11), Emily Launer (11), Katherine Hare (11) | Rosary High School | Aurora | IL |
| 59 | 3:30.72 | Allison Viverette (12), Natalie Feola (9 ), Shannon Haberman (11), Leah Smith (10) | Oakland Catholic High School | Pittsburgh | PA |
| 60 | 3:30.73 | Casey Clark (12), Jordan Domeier (10), Jordan Smith (11), Katelyn Crawford (10) | Klein High School | Spring | TX |
| 61 | 3:30.78 | Camey Rabold (11), Emily Boone (12), Michele Rielly (11), Samantha Schuttinger (10) | Bishop Watterson High School | Columbus | OH |
| 62 | 3:30.85 | Sami Pochowski (11), Emma Szczupakiewicz (11), Megan Liebl (10), Kristen Kaiser (10) | Muskego High School | Muskego | WI |
| 63 | 3:30.95 | Emma O'Connell (12), Erin Oeltjen (10), Karen Illg (12), Elizabeth McGinn (12) | Millard West High School | Omaha | NE |
| 64 | 3:31.00 | Lindsey Schmidt (11), Sam Wheatley (9 ), Erin Regan (12), Carolyn Meier (11) | Upper Dublin High School | Fort Washington | PA |
| 65 | 3:31.19 | Summer Stephens (12), Rebecca Sears (11), Ashley LeClair (9 ), Elly Faulkner (12) | Sacred Heart Academy | Louisville | KY |
| 66 | 3:31.25 | Abby Puczkowski (12), Julia Farfan (10), Haley Preston (11), Savannah Miller (11) | Westlake High School | Westlake Village | CA |
| 67 | 3:31.29 | Samantha Starnes (10), Hannah Pinion (12), Whitney Owen (10), Ally Hardesty (11) | Bearden High School | Knoxville | TN |
| 68 | 3:31.31 | Margaret Cooke (10), Carly Pratt (9 ), Victoria Eberwein (9 ), Erin Foster (12) | The Woodlands High School | The Woodlands | TX |
| 69 | 3:31.40 | Fotini Kaufman (11), Kristen Worthington (11), Jennifer Coady (11), Genevieve Heidkamp (11) | Hinsdale Central High School | Hinsdale | IL |
| 69 | 3:31.40 | Rachel Hayden (9 ), Mary Grace Van Allen (10), Anna Dehnke (11), Amy Katz (12) | North Central High School | Indianapolis | IN |
| 71 | 3:31.54 | Ashlyn Kiletic (11), Cara Gilligan (11), Elsa Welshofer (9 ), Lauren Rhodes (9 ) | Charlotte Catholic High School | Charlotte | NC |
| 72 | 3:31.56 | Abigail Meyer (9 ), Koryn Ternes (11), Erin Flanigan (12), Nadia El-Hamdi (12) | Bowie High School | Austin | TX |
| 73 | 3:31.60 | Brittany Gilbert (11), Elaine Pitsas (12), Mary Beth Blocher (9 ), Jillian Homans (11) | Munster High School | Munster | IN |
| 73 | 3:31.60 | Allison Deperalta (11), Kelsey Kafka (10), Anique Hermann (12), Margaret Guo (11) | Torrey Pines High School | San Diego | CA |
| 75 | 3:31.63 | Riley Schroedter (10), Megan Childs (10), Amanda Childs (10), Gia Dalesandro (11) | Neuqua Valley High School | Naperville | IL |
| 76 | 3:31.64 | Catherine Breed (12), Kirsten Brand (10), Tory Houston (12), Natalie Wong (12) | Amador Valley High School | Pleasanton | CA |
| 77 | 3:31.74 | Cecelia Williams (9 ), Alyssa Yambor Maul (10), Lia Lombardi (10), Delaney Barnard (9 ) | Lawton Chiles High School | Tallahassee | FL |
| 78 | 3:31.89 | Brittany Kamper (10), Tara Capouch (11), Caitlin Krull (9 ), Kim Katner (10) | Carl Sandburg High School | Orland Park | IL |
| 79 | 3:31.91 | Sommers Creed (12), Frederique Lefebvre (11), Maddie Wrolson (9 ), Rachael Dudley (11) | Mill Creek High School | Hoschton | GA |
| 80 | 3:32.01 | Makayla Myers (9 ), Danielle Freeman (10), Audra Thornton (11), Michaela Rookus (11) | Hudsonville High School | Hudsonville | MI |
| 81 | 3:32.02 | Tara Rogers (9 ), Brooke Else (11), Eve Kosten (9 ), Jan Ikeda (12) | North Penn High School | Lansdale | PA |
| 82 | 3:32.16 | Morgan Karetnick (11), Kim Hill (11), Jesse Evans (12), Erin Calderoni (12) | Greenwich High School | Greenwich | CT |
| 82 | 3:32.16 | Maria Bernard (12), Gabriella Gil (11), Riki Bonnema (11), Rebecca Bernard (12) | Olympia High School | Orlando | FL |
| 84 | 3:32.17 | Melanie McDonough (12), Lauren Jones (10), April McDonough (9 ), Whitney Allen (11) | Frisco High School | Frisco | TX |
| 85 | 3:32.20 | Taylor Katz (10), Meghan Buckley (12), Niamh O'Grady (9 ), Cha Cha Bugatti (12) | Riverview High School | Sarasota | FL |
| 86 | 3:32.22 | Holly Moon (12), McKenzie Calkins (12), Maya Owens (11), Danielle Spradlin (12) | Converse Judson High School | Converse | TX |
| 87 | 3:32.37 | Alina Schulhofer (10), Rebecca Aiello (12), Hannah Veale (9 ), Brittany Hammond (10) | Pine Crest School | Fort Lauderdale | FL |
| 88 | 3:32.64 | Allie Zeidan (11), Jayme Katis (12), Abi Speers (10), Meghan Overend (12) | Langley High School | McLean | VA |
| 89 | 3:32.67 | Alex Untied (9 ), Sydney King (9 ), Kelsey Haller (11), Lori Davis (11) | Granville High School | Granville | OH |
| 90 | 3:32.68 | Sally Thompson (10), Alex Schuttinger (9 ), Chelsea Staab (12), Taylor Schwartz (11) | Westerville Central High School | Westerville | OH |
| 91 | 3:32.69 | Casey Francis (10), Megan Brady (11), Frances Warren (12), Tan Emily (12) | Sacred Heart Academy | Hempstead | NY |
| 92 | 3:32.74 | Dina Rommel (9 ), Victoria Butler (9 ), Sydney Modeas (9 ), Rebecca Anthone (9 ) | Clarence High School | Clarence | NY |
| 93 | 3:32.78 | Janet Hu (9 ), Stephanie Hallock (11), Kendall Lawhorn (9 ), Kaitlin Pawlowicz (12) | Oakton High School | Vienna | VA |
| 94 | 3:32.81 | Allison Ridge (11), Kaitlyn Ferrara (10), Megan Daniher (12), Betsy Zilch (11) | St. Ursula Academy | Cincinnati | OH |
| 95 | 3:32.82 | Ryan Alexander (9 ), Hannah Verrette (9 ), Toireasa Rafferty-Millet (11), Megan Alexander (11) | Marblehead High School | Marblehead | MA |
| 96 | 3:32.88 | Emily Pia (11), Kellie Gervas (12), Kara Kaulius (10), Ashley Niznik (11) | West Allegheny High School | Imperial | PA |
| 97 | 3:32.89 | Olivia Smoliga (10), Anna Veselovsky (11), Hallie Hengels (11), Niki Wells (10) | Glenbrook South High School | Glenview | IL |
| 98 | 3:32.99 | Valerie Borger (11), Morgan Contino (10), Stephanie Pearce (12), Molly Hazelbaker (12) | Turpin High School | Cincinnati | OH |
| 99 | 3:33.00 | Stephanie Nemeth (11), Rachel Ciferno (11), Jacquelynn Anderson (12), Alana Arnold (12) | Mayfield High School | Mayfield Village | OH |
| 100 | 3:33.01 | Hannah Newell (9 ), Taylor Flummerfelt (10), Rachel Shriver (11), Maddie Bro (11) | Ames High School | Ames | IA |