| Place | Time | Relay | School | City | State |
| 1 | 2:54.43 | Ryan Murphy (12), Santo Condorelli (12), Josh Booth (11), Joseph Schooling (11) | The Bolles School | Jacksonville | FL |
| 2 | 3:00.23 | Michael Jensen (9 ), Ian Flynn (12), Eric Jensen (12), Robert Bantley (12) | Upper Dublin High School | Fort Washington | PA |
| 3 | 3:00.56 | Clark Smith (12), Hennessey Stuart (11), Erik Biernat (12), Zach Suter (12) | Regis Jesuit High School | Aurora | CO |
| 4 | 3:00.89 | Jackson Locke (12), Tim Kou (11), Chris Scheaffer (12), Aidan Burns (10) | Bellarmine College Preparatory | San Jose | CA |
| 5 | 3:01.05 | Blake Pieroni (11), Ethan Whitaker (11), Patrick Curley (11), Aaron Whitaker (11) | Chesterton High School | Chesterton | IN |
| 6 | 3:01.70 | Grant Sivesind (11), Steven Stumph (12), J D Ratchford (12), Sven Campbell (12) | Campolindo High School | Moraga | CA |
| 7 | 3:01.73 | Trent Trump (11), Nick Silverthorn (11), Maxime Rooney (9 ), Bryce McLaggan (11) | Granada High School | Livermore | CA |
| 8 | 3:02.02 | Jonathan DesCombes (12), Rafael Felix (10), Jonathan Beck (12), Spencer DeShon (12) | Capistrano Valley High School | Mission Viejo | CA |
| 9 | 3:02.71 | Sam Coffman (10), Jake Markham (11), Daryl Turner (12), John Mattern (12) | Cherry Creek High School | Englewood | CO |
| 10 | 3:02.75 | Jack Mangan (12), Brian Walsh (12), Jae Park (11), Reed Malone (12) | New Trier High School | Winnetka | IL |
| 11 | 3:03.78 | Gust Kouvaris (10), Patrick Nodland (12), Mark Blinstrub (10), Joseph Krause (11) | Brother Rice High School | Bloomfield Hills | MI |
| 12 | 3:04.01 | Colin Rowe (12), Bogdan Balteanu (12), Daniel Smith (10), Peter Grumhaus (12) | Lake Forest High School | Lake Forest | IL |
| 13 | 3:04.10 | Aaron Greenberg (12), Mike Solfelt (12), Bryce Boston (12), Jonathan Lieberman (12) | Eden Prairie High School | Eden Prairie | MN |
| 13 | 3:04.10 | Jonathan Roberts (11), Grayson Magruder (11), John Remetta (12), Jacob Tovey (12) | Southlake Carroll High School | Southlake | TX |
| 15 | 3:04.14 | Jared Strong (11), Nick Bell (12), Michael Forbes (11), Kyle Coan (11) | Los Osos High School | Rancho Cucamonga | CA |
| 16 | 3:04.27 | Cannon Clifton (12), Ryan Sorensen (10), Dusan Vukobrat (12), John Sanden (11) | The Woodlands High School | The Woodlands | TX |
| 17 | 3:04.73 | Greg Brocato (9 ), Sean Regan (12), Kyle McElwee (10), Evan Holder (12) | LaSalle College High School | Wyndmoor | PA |
| 18 | 3:04.86 | Nathan Christian (11), Peter Simcox (11), Charlie Pophal (12), Derek Hren (12) | Saint Ignatius High School | Cleveland | OH |
| 19 | 3:04.89 | Jeremy Temprano (12), Anthony Walker (12), John Hornacek (11), Nick Menninga (12) | James Madison Memorial High School | Madison | WI |
| 20 | 3:05.00 | Gage Kohner (11), Nicholas Van Zee (12), Kevin Matheus (12), Jason McCormick (12) | Boca Raton High School | Boca Raton | FL |
| 21 | 3:05.04 | Curtis Ogren (11), Reid Lazzarini (12), Sean Elmurib (10), Benjamin Ho (9 ) | Saint Francis High School | Mountain View | CA |
| 22 | 3:05.07 | Bryan Hughes (12), Nick Johnston (12), Jason Chen (12), Kevin McLaughlin (11) | Amador Valley High School | Pleasanton | CA |
| 23 | 3:05.20 | Jack Conger (12), Matt Colket (12), Brady Welch (12), Tom Colket (12) | Our Lady of Good Counsel | Olney | MD |
| 23 | 3:05.20 | Tyler Martin (12), Clayton Peterson (11), Nick Pasichuke (12), Clayton Young (12) | San Ramon Valley High School | Danville | CA |
| 25 | 3:05.23 | Josh Schechter (12), Logan Lagesse (12), Ethan Pfeiffer (12), Kyle Hentschel (12) | Arapahoe High School | Centennial | CO |
| 26 | 3:05.53 | Liam Karas (11), Jack Harryman (12), Rich Barden (12), Tyler Lin (10) | Corona del Mar High School | Newport Beach | CA |
| 27 | 3:05.60 | Patrick Conaton (11), Richard Mannix (11), Declan Kennon (11), Joe Eiden (12) | Fordham Preparatory School | Bronx | NY |
| 27 | 3:05.60 | Yu-Bin Kim (11), Ryan Lee (11), David Puczkowski (10), Michael Wen (12) | Westlake High School | Westlake Village | CA |
| 29 | 3:05.68 | Jacob Molacek (11), Ryan Tate (10), Mike Bauwens (11), Sean Tate (10) | Creighton Preparatory School | Omaha | NE |
| 30 | 3:05.75 | Gable Ostler (11), Albert Miao (12), Miles Kalbus (11), Charlie Wiser (12) | Miramonte High School | Orinda | CA |
| 31 | 3:05.80 | Jeremiah Morren (12), Derek Bosko (12), Thomas Rathbun (11), Kyle Doss (12) | Holland High School | Holland | MI |
| 32 | 3:05.92 | Brian Giannetti (12), Stephen Watson (11), Drew Bercaw (12), Robert Tolson (12) | North Canton Hoover High School | North Canton | OH |
| 33 | 3:05.97 | Jack Hendricks (12), Mitchell Frey (10), Ian Wooley (12), Grant Johnson (12) | Saint Xavier High School | Cincinnati | OH |
| 34 | 3:06.00 | Buzz Rehnberg (11), Sean Satterthwaite (12), Jack Lindell (12), Erick Huft (12) | Edina High School | Edina | MN |
| 35 | 3:06.12 | Daniel Bridgers (12), Zach Marshall (11), Zach Bunner (12), Jimmy Yoder (12) | Lassiter High School | Marietta | GA |
| 36 | 3:06.15 | Mike Burke (11), Josh Kanute (12), Jonathan Thielen (12), Robert Ramoska (12) | Marmion Academy | Aurora | IL |
| 37 | 3:06.17 | Harry Sale (10), Andrew Reynolds (12), Todd Ber (9 ), Marshall Pringle (12) | Klein High School | Klein | TX |
| 38 | 3:06.21 | Quinn Cassidy (12), Luke Hayhoe (11), Nicholas Conrad (10), Shawn Warner (12) | Saint Andrew's School | Boca Raton | FL |
| 39 | 3:06.23 | Matt Whittle (11), Andrew Block (12), Jonathan Pun (11), Riley Hickman (10) | Davis Senior High School | Davis | CA |
| 40 | 3:06.37 | Max McKay (12), Mitch Cooper (11), Ben Burdick (11), Jon Astashinsky (12) | Carmel High School | Carmel | IN |
| 41 | 3:06.54 | Alec Willrodt (12), Cai Rohleder (12), Kyle Rathgeb (12), Michael Miller (12) | Stratford High School | Houston | TX |
| 42 | 3:06.59 | Robbie Newman (10), Chase Vennefron (11), Mike Summe (10), Max Williamson (12) | Covington Catholic High School | Park Hills | KY |
| 43 | 3:06.61 | Zach Luttrell (11), Jeremy Bauchwitz (12), Will Drexler (10), Vinny Tafuto (12) | Hershey High School | Hershey | PA |
| 44 | 3:06.69 | Will Peterson (10), Ian Keane (11), Christopher Wieser (11), Keegan Shuping (11) | Jesuit High School | Carmichael | CA |
| 44 | 3:06.69 | Kaikena Naone (12), Kanoa Kaleoaloha (10), Kale Ai (12), Ryan Stack (11) | Kamehameha Schools Kapālama High School | Honolulu | HI |
| 46 | 3:06.82 | M P Salinas (12), Billy Beard (9 ), Christopher Cary (11), Erik Dresden (11) | Malvern Preparatory School | Malvern | PA |
| 47 | 3:06.84 | Gabriel Espinosa (12), Brad Dorsey (11), Ryan McCoy (11), Lukas Williams (12) | Brophy College Preparatory | Phoenix | AZ |
| 47 | 3:06.84 | Matthew Horner (10), Thomas Donovan (12), Garrett Middleton (11), Jackson Miller (12) | Las Lomas High School | Walnut Creek | CA |
| 49 | 3:06.94 | Tristan Sanders (11), Manuel Barragan (11), Kristopher Hoce (11), Ryan McRae (12) | Palm Harbor University High School | Palm Harbor | FL |
| 50 | 3:06.95 | Nehemiah Mork (9 ), Michael Pixton (12), Benjamin Martin (12), Jackson Goethe (12) | H. H. Dow High School | Midland | MI |
| 51 | 3:07.03 | Quinn Smith (11), Matt Hrabchak (10), Miles Smachlo (9 ), Oscar Miao (12) | Deerfield Academy | Deerfield | MA |
| 52 | 3:07.07 | Jackson O'Dowd (9 ), John Ferrara (12), Parker Belmore (11), Nick Arakelian (11) | Stevenson High School | Livonia | MI |
| 53 | 3:07.11 | Alex Hardwick (11), Nathan Novak (11), Nikhil Narayanan (11), Ryan Dudzinski (10) | Upper St. Clair High School | Pittsburgh | PA |
| 54 | 3:07.16 | Connor Schroyer (12), Banistre Lienhart (11), Brian Portland (10), Will Leach (12) | Hinsdale Central High School | Hinsdale | IL |
| 55 | 3:07.19 | Jake Goroshko (10), Geoffrey Boodey (12), Steve Sekula (12), Brandon McCredie (11) | Council Rock High School North | Newtown | PA |
| 55 | 3:07.19 | Max Phillips (12), Miles MacKenzie (11), Alex Walton (11), Connor Corrigan (12) | Fairview High School | Boulder | CO |
| 57 | 3:07.23 | Max Staresinic (10), Zachary Buerger (11), Greg Arnold (10), Sam Rutan (11) | North Allegheny Senior High School | Wexford | PA |
| 58 | 3:07.34 | Andrew Liang (11), William Lee (11), Andrew Cho (9 ), Alex Francis (12) | Palo Alto High School | Palo Alto | CA |
| 59 | 3:07.44 | Kyle Otazu (11), Josh Kim (9 ), Sam Jo (9 ), Peter Kropp (12) | Loyola High School | Los Angeles | CA |
| 60 | 3:07.51 | Spencer Gray (12), Nick Kowaleski (12), Stephen Hutchinson (9 ), Kyle Gannon (12) | St. Charles North High School | St. Charles | IL |
| 61 | 3:07.66 | Jeremy Estes (12), Sam Sauerbrei (10), Jared Smith (11), Joe Gebhart (11) | Boulder City High School | Boulder City | NV |
| 62 | 3:07.67 | Adam Whitener (12), Michael Bundas (12), David Boland (12), Joshua Ehrman (12) | Saline Area Schools | Saline | MI |
| 63 | 3:07.72 | Jonathon DiSalle (11), Matthew Gustafson (12), John Barone (11), Nicholas Brodie (11) | St. Francis DeSales High School | Toledo | OH |
| 64 | 3:07.74 | Sam Hoon Lee (11), Corey Okubo (11), Zachary Yong (12), Matthew Wong (10) | University High School | Irvine | CA |
| 65 | 3:07.76 | Jeff Wynkoop (11), Kyle Cude (10), Brandon Boval (11), Taylor Peterson (12) | Tesoro High School | Las Flores | CA |
| 66 | 3:07.79 | Brandon Pon (11), Colin Hong (11), Evan Li (12), Kevin Su (12) | Monta Vista High School | Cupertino | CA |
| 67 | 3:07.83 | Enrique Hernandez (10), Connor Saeli (12), Michael Shaben (11), Addison Williams (12) | Birmingham Seaholm High School | Birmingham | MI |
| 67 | 3:07.83 | Sam Loescher (11), Sam Parker (12), Matt Freudenthaler (10), Cody Taylor (11) | Columbus North High School | Columbus | IN |
| 69 | 3:07.87 | Matthew Harrington (10), Justin Fu (10), Atticus Rush (11), Alex Snarski (11) | Libertyville High School | Libertyville | IL |
| 70 | 3:07.89 | Bailey Biwer (11), James Olson (12), Trevor Manz (12), Alex DeLakis (12) | Eau Claire Memorial North High School | Eau Claire | WI |
| 71 | 3:07.91 | Alex Garriga (10), Clay McAnany (12), Joseph Tak (11), Andrew Skowronek (12) | Ronald Reagan High School | San Antonio | TX |
| 72 | 3:07.93 | Mitchel Stahara (11), Zach Misak (12), Daniel Litwin (12), Erik Brinkhoff (12) | Bethel Park Senior High School | Bethel Park | PA |
| 73 | 3:07.94 | Joe Faller (11), Scott Simpson (11), Travis Bouscaren (11), Tim Wynter (11) | Phillips Academy | Andover | MA |
| 74 | 3:07.96 | Ben Wurst (11), Matthew Fraser (12), Alexander Lewis (11), Thomas Dillinger (10) | Greenwich High School | Greenwich | CT |
| 75 | 3:08.01 | Johnathan Zoucha (11), Jordan Hurwitz (12), Tristan Furnary (9 ), Cameron Stitt (12) | Sunset High School | Portland | OR |
| 76 | 3:08.02 | Brennan Maisch (12), Maxwell Merriman (12), Robert Zofchak (9 ), Jonathan Zofchak (12) | Dexter High School | Dexter | MI |
| 77 | 3:08.03 | Alec Niederland (11), Nathan Torres (11), Thomas Gillespie (11), Thomas Smith (10) | Edison High School | Huntington Beach | CA |
| 78 | 3:08.19 | Kai Williams (10), Daniel Wu (11), Davis Argersinger (12), Chris Klein (12) | Ann Arbor Pioneer High School | Ann Arbor | MI |
| 79 | 3:08.23 | Kyle Gornay (11), Shea Macready (10), Roy Diego McCoy (12), Trevor Togashi (12) | Redlands High School | Redlands | CA |
| 80 | 3:08.24 | Christopher Kearney (9 ), Jack Considine (12), Blake Morgan (12), Andrew Jovanovic (12) | Loyola Academy | Wilmette | IL |
| 81 | 3:08.26 | Sam McHugh (11), Christian Selby (10), Will Raines (10), Chandler Hinson (11) | The Baylor School | Chattanooga | TN |
| 82 | 3:08.27 | Cole Buese (10), John Clancy (11), Daniel Moreno (11), Dominic Breschi (12) | Loyola Blakefield | Towson | MD |
| 83 | 3:08.37 | Spencer Adickes (11), Austin Frey (12), Christopher Spears (12), Travis Lipscomb (12) | Alamo Heights High School | San Antonio | TX |
| 83 | 3:08.37 | Jack Herron (10), Josh Cutter (12), Shane Anderson (10), Tyler Cutter (10) | Bear River High School | Grass Valley | CA |
| 85 | 3:08.41 | Carsten Vissering (10), Joey Snodderly (10), Adrian Lin (10), Grant Goddard (10) | Georgetown Preparatory School | Rockville | MD |
| 86 | 3:08.45 | Samuel Lorentz (12), Joseph Steinkamp (12), Joseph Krahulik (11), Theodore Steinkamp (12) | North Central High School | Indianapolis | IN |
| 87 | 3:08.46 | Brayden Seal (11), Samuel Kline (9 ), Coleman Phelps (12), Zachary Kline (12) | Frisco Wakeland High School | Frisco | TX |
| 88 | 3:08.52 | Seth Wenner (12), Ben Johnston (10), Zachary Snyder (9 ), Jack Brenneman (11) | Cumberland Valley High School | Mechanicsburg | PA |
| 88 | 3:08.52 | Blake Franklin (12), Jordan Weaver (11), Jacob Kelsoe (12), Ethan King (12) | Memphis Home Education Association | Memphis | TN |
| 90 | 3:08.61 | Ali Khalafalla (11), Michael Guerci (12), Karim Elsayed (11), Mohamed Saleh (12) | Fork Union Military Academy | Fork Union | VA |
| 91 | 3:08.67 | Timothy Martin (11), Evan Shive (10), Karol Schmuck (11), Josh Phelps (12) | Jeffersonville High School | Jeffersonville | IN |
| 92 | 3:08.69 | Jack Boyd (11), Andrew Wojtowicz (10), William McCauley (12), Christian Westermeier (12) | Canisius High School | Buffalo | NY |
| 93 | 3:08.70 | Adam Drury (11), Steven Fishman (11), John Remmes (11), Jake Miller (10) | University High School | Normal | IL |
| 94 | 3:08.75 | Arjun Sharma (10), Grant Watson (9 ), Matthew Lennon (10), Talbot Jacobs (11) | The College Preparatory School | Oakland | CA |
| 95 | 3:08.77 | Phillip Ugarte (11), Andrew Byerly (11), Anthony Hauglum (10), Oliver Garnier (12) | Flour Bluff High School | Corpus Christi | TX |
| 96 | 3:08.79 | Thomas Jones (11), Johann Shim (12), James Jones (9 ), James Murphy (9 ) | Robinson Secondary School | Fairfax | VA |
| 97 | 3:08.80 | Jack Strauss (12), Kyle Ujiiye (12), Sebastian Piekarski (11), Chris Vega (12) | Barrington High School | Barrington | IL |
| 98 | 3:08.87 | Nikita Bondarenko (12), Nathan Pimentel (12), Kyle Patnode (12), Mark Hallman (11) | Oak Park and River Forest High School | Oak Park | IL |
| 99 | 3:09.07 | Miles Oakley (11), Emilio Sison (9 ), Luke Thorsell (10), Andrew Seliskar (10) | Thomas Jefferson High School For Science and Tech | Alexandria | VA |
| 100 | 3:09.10 | Grant Gillenwater (12), Len Sterle (12), Zack Milenius (12), Justin Ciferno (11) | Mayfield High School | Mayfield Village | OH |